The Protective Effects of Fasciotomy on Reperfusion Injury of Skeletal Muscle of Rabbits

Abstract

The authors aim to investigate protective effects of fasciotomy against ischemia reperfusion injury of skeletal muscle in rabbit and to compare the treatment effects of prereperfusion + fasciotomy and fasciotomy + postreperfusion against ischemia reperfusion injury of skeletal muscle. 24 healthy male Japanese white rabbits were randomly divided into 3 groups, and 4 hours' ischemia was established in these rabbits through surgery. Six hours' reperfusion was performed in group A; reperfusion + postfasciotomy was performed in group B; and prefasciotomy + reperfusion was performed in group C. Result showed that prefasciotomy and postfasciotomy could protect skeletal muscle against ischemia reperfusion injury, reduced MDA (malondialdehyde) expression, MPO (myeloperoxidase) expression, and apoptosis of muscle in the reperfused areas, increased Bcl-2 expression, and decreased Bax expression. The MDA and MPO levels in group B and group C were significantly lower than those in group A, and MDA and MPO levels in group C were significantly lower than those in group B. Prefasciotomy and postfasciotomy could protect against ischemia reperfusion injury in skeletal muscle. The protective effects of prefasciotomy against ischemia reperfusion injury are better than postfasciotomy.

Remarkable Protective Effects of Nrf2-Mediated Antioxidant Enzymes and Tissue Specificity in Different Skeletal Muscles of Daurian Ground Squirrels Over the Torpor-Arousal Cycle

“…Under conditions of excessive ROS, organismal endogenous antioxidant defenses are initiated to maintain redox homeostasis; however, if the accumulation of ROS exceeds the body’s antioxidant elimination capacity, oxidative damage can occur (Espinosa-Diez et al, 2015). Previous studies have found that malondialdehyde (MDA), a biomarker of lipid peroxidation, significantly increases in the skeletal muscles of non-hibernating rats (Lin et al, 2018) and in the serum and hindlimb skeletal muscles of non-hibernating rabbits following ischemia-reperfusion (Li et al, 2017; Zhang et al, 2018). Furthermore, the activities of multiple antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx), are significantly reduced in the skeletal muscles of non-hibernating rabbits experiencing ischemia-reperfusion (Zhang et al, 2018), with changes also observed in apoptosis gene expression in skeletal muscle fibers (e.g., increased Bax and decreased Bcl-2 expression) (Li et al, 2017; Zong et al, 2017).…”

P-Kumari̇k Asi̇t, Renal İskemi̇ Reperfüzyon Kaynakli Akut Pulmoner Hasari Azaltir

“…44 MPO, nötrofi l azurofi lik granüller ve makrofaj hücrelerinde bulunan bir kan proteinidir ve MPO seviyesi, doku hasar bölgesinde nötrofi llerin infi ltrasyon derecesini yansıtabilir. 45,46 Özetle; sunulan bu çalışma ile renal iskemi reperfüzyon kaynaklı akut akciğer hasarı ve bu hasara karşı alternatif tedavi yaklaşımı olarak p-CA'nın oksidan-antioksidan denge üzerine etkisi biyokimyasal olarak incelendi. Bu molekülün böbrek IR hasarında uzak doku etkileri ile ilişkili olarak akut akciğer hasarına etkisi literatürde bulunmadığından sunulan çalışmamız özgündür.…”