The Protective Effects of Astaxanthin on the OVA-Induced Asthma Mice Model

Abstract

Although astaxanthin has a variety of biological activities such as anti-oxidant effects, inhibitory effects on skin deterioration and anti-inflammatory effects, its effect on asthma has not been studied. In this paper, the inhibitory effect of astaxanthin on airway inflammation in a mouse model of ovalbumin (OVA)-induced asthma was investigated. We evaluated the number of total cells, Th1/2 mediated inflammatory cytokines in bronchoalveolar lavage fluid (BALF) and airway hyperresponsiveness as well as histological structure. The level of total IgE, IgG1, IgG2a, OVA-specific IgG1, and OVA-specific IgG2a were also examined. The oral administration of 50 mg/mL astaxanthin inhibited the respiratory system resistance, elastance, newtonian resistance, tissue damping, and tissue elastance. Also, astaxanthin suppressed the total cell number, IL-4, and IL-5, and increased the IFN-γ in the BALF. In the sera, total IgE, IgG1, and OVA-specific IgG1 were reduced by astaxanthin exposure and IgG2a and OVA-specific IgG2a were enhanced via oral administration of astaxanthin. Infiltration of inflammatory cells in the lung, production of mucus, lung fibrosis, and expression of caspase-1 or caspase-3 were suppressed in OVA-induced asthmatic animal treated with astaxanthin. These results suggest that astaxanthin may have therapeutic potential for treating asthma via inhibiting Th2-mediated cytokine and enhancing Th1-mediated cytokine.

Kadmiyum ile Oksidatif Strese Maruz Kalan Sıçanların Beyin Dokusunda Astaksantinin Etkisi;Biyokimyasal, Histopatolojik Değerlendirme

“…Therefore, important sources of Cd intake are via food or cigarette (Yadav et al, 2010).Prolonged exposure to Cd causes toxic effects on cardiovascular, immune/ hematopoietic, skeletal, endocrine and reproductive systems and also kidneys, liver, brain and lungs (Fowler, 2009;Satarug et al, 2010).Cd accumulation causes serious toxic effects in brain (Karoui-Kharrat et al, 2017) as well as other neurological disorders such as hyperactivity, memory loss and learning toughness (Hart et al, 1989;Ciesielski et al,2012;Szkup et al, 2012).Since brain has lower activity in enzymatic defense systems match other organs it is indefensible oxidative stress (Shagirtha et al, 2016). Astaxanthin (3,30-dihydroxy-β, β-carotene-4,40dion) is one of the xanthophyll carotenoids found mostly in microalgae, fungi, complex plants, seafood, flamingos and quails (Hwang et al, 2017). Astaxanthin has some a wide range of biological activities such as including antioxidant,anti-inflammatory, anticancer and cardioprotective activities (Wu et al, 2012).…”

Role of Th2 cytokines on the onset of asthma induced by meta‐xylene in mice

“…Cytokines and immunoglobulin were obtained at the place where they were usually measured . BALF supernatant and serum were stored at −80°C.…”

“…As described in previous studies, AHR was measured in anesthetized mice within 24 hours of the final challenge . An 18G metal cannula was inserted into the trachea to expose the methacholine (MCH).…”

“…Cytokines and immunoglobulin were obtained at the place where they were usually measured. [21][22][23] BALF supernatant and serum were stored at −80 C. The levels of IL-4, IL-5, IL-13 and IFN-γ in BALF and the levels of total IgE in serum were determined by ELISA.…”

“…As described in previous studies, AHR was measured in anesthetized mice within 24 hours of the final challenge. 21,22 An 18G metal cannula was inserted into the trachea to expose the methacholine (MCH). The mice were placed in a flow-type body plethysmograph and connected to a computer-controlled ventilator (FlexiVent, SCIREQ, Inc., Montreal, Canada).…”