The Protective Effect of Semax in a Model of Stress-Induced Impairment of Memory and Behavior in White Rats

Glazova, N. Yu.; Sebentsova, E. A.; Manchenko, D. M.; Андреева, Л. А.; Dergunova, Lyudmila V.; Levitskaya, N. G.; Limborska, S. A.; Myasoedov, N. F.

doi:10.1134/s1062359018040040

Cited by 7 publications

(16 citation statements)

References 21 publications

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“…For example, here we used Semax and emotional somatosensory stress model. No effects of Semax on stress‐induced anxiety were obtained in vivo as well as in vitro (for [ 3 H]GABA binding), but the cognitive effects of Semax in above model were present. In this light, we can choose one of two (or both) most obvious next steps: retain the model but change the ligand and receptor system (use, for example, [ 3 H]Glu, or [ 3 H]Dopa, or synthetic effector molecule), or retain the ligand but change the model.…”

Section: Discussionsupporting

confidence: 54%

“…In particular, the above complex stress led to memory impairment against the background of anxiety decrease. Preliminary intraperitoneal administration of Semax (0.1 mg/kg) decreases cognitive impairment, but does not affect stress‐induced anxiety . The above correlates well with the observed ligand‐receptor analysis data.…”

Section: Discussionmentioning

confidence: 99%

“…(A more detailed description is given in). The study was performed on male Wistar rats weighing 230 to 250 g. The animals were housed in standard laboratory conditions under a 12: 12‐hour light/dark cycle with free access to water and food (except for the learning period).…”

Section: Methodsmentioning

confidence: 99%

“…Sebentsova, and N.G. Levitskaya at the Biological Faculty of Lomonosov State University (Moscow) for preparing the animals for the experiments (developed a morbid state model) and collecting the corresponding rat brain tissues.…”

Section: Acknowledgementsmentioning

confidence: 99%

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An integrated approach to study the molecular aspects of regulatory peptides biological mechanism

V’yunova

Andreeva

Шевченко

et al. 2019

Labelled Comp Radiopharmac

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An integrated methodological approach to study the molecular aspects of short regulatory neuropeptides biological mechanism is proposed. The complex research is based on radioligand-receptor method of analysis and covers such points of peptides molecular activity as: specific binding of peptides to brain cells plasmatic membranes, formation of tissue specific synacton, influence of peptides (as allosteric modulators) on functionality of different neuroreceptors as well as delayed in time effects of peptides on receptor-binding activity of well-known neuroreceptor systems. Radiolabeled ligands in such complex study are the one of the best and precision instruments to uncover the molecular mechanism of multiple and multitarget biological effects of regulatory peptides. In this issue we used heptapeptide Semax as a model regulatory peptide, [ 3 H]Ach and [ 3 H]GABA as an effector molecules, and the rat model of stress-induced memory and behavior impairment as a morbid state. We showed the ability of Semax to modulate in a dose-dependent manner [ 3 H] Ach and [ 3 H]GABA specific binding to some of its corresponding receptors as well as to affect the number of [ 3 H]GABA specific binding places on rat neurons plasmatic membranes after complex stress exposure.

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Section: Discussionsupporting

confidence: 54%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Acknowledgementsmentioning

confidence: 99%

See 2 more Smart Citations

An integrated approach to study the molecular aspects of regulatory peptides biological mechanism

V’yunova

Andreeva

Шевченко

et al. 2019

Labelled Comp Radiopharmac

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“…It has been shown that Semax improves cognitive function in both rodents and humans. The peptide facilitates acquisition of food-motivated and passive-avoidance tasks in healthy animals and has a protective effect in a model of stress-induced memory impairment in rats [ 9 , 10 , 11 ]. In experimental models of cerebral ischaemia Semax administration led to the recovery of the animals’ ability to learn in a Morris water maze and passive-avoidance task [ 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

Novel Insights into the Protective Properties of ACTH(4-7)PGP (Semax) Peptide at the Transcriptome Level Following Cerebral Ischaemia–Reperfusion in Rats

Filippenkov

Stavchansky

Denisova

et al. 2020

Genes

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Cerebral ischaemia is the most common cause of impaired brain function. Biologically active peptides represent potential drugs for reducing the damage that occurs after ischaemia. The synthetic melanocortin derivative, ACTH(4-7)PGP (Semax), has been used successfully in the treatment of patients with severe impairment of cerebral blood circulation. However, its molecular mechanisms of action within the brain are not yet fully understood. Previously, we used the transient middle cerebral artery occlusion (tMCAO) model to study the damaging effects of ischaemia–reperfusion on the brain transcriptome in rats. Here, using RNA-Seq analysis, we investigated the protective properties of the Semax peptide at the transcriptome level under tMCAO conditions. We have identified 394 differentially expressed genes (DEGs) (>1.5-fold change) in the brains of rats at 24 h after tMCAO treated with Semax relative to saline. Following tMCAO, we found that Semax suppressed the expression of genes related to inflammatory processes and activated the expression of genes related to neurotransmission. In contrast, ischaemia–reperfusion alone activated the expression of inflammation-related genes and suppressed the expression of neurotransmission-related genes. Therefore, the neuroprotective action of Semax may be associated with a compensation of mRNA expression patterns that are disrupted during ischaemia–reperfusion conditions.

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Synthetic corticotropins and the GABA‐receptor system: Direct and delayed effects

et al. 2023

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The central effectors of the stress system are greatly interconnected and include, among others, a large group of peptides derived from proopiomelanocortin. In addition to natural corticotropins, a number of artificial molecules that contain some ACTH fragments in their structure are also referred to members of this family. Some of them possess a wide range of biological activity. The molecular mechanism underlying the biological activity of such peptides is partly based on allosteric modulation of various receptors. We analyzed the ability of some biologically active synthetic corticotropins (ACTH(4‐7)PGP, ACTH(6‐9)PGP, ACTH(7‐10)PGP), and glyproline PGPL to affect the GABA‐receptor system of rat brain. The effects of the peptides were studied in the isolated plasma membranes of brain cells, as well as after systemic peptide administration in the rat model of acute restraint stress. The delayed effect of stress or preadministration of each of the studied peptides on [3H]GABA binding was different for its high‐ and low‐affinity‐specific sites. The studied peptides individually affected the binding of [3H]GABA in their own way. Acute restraint stress caused a decrease in [3H]GABA binding at its low‐affine site and did not affected the high‐affine site. Preliminary peptide administration did not influence this effect of stress.

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The Protective Effect of Semax in a Model of Stress-Induced Impairment of Memory and Behavior in White Rats

Cited by 7 publications

References 21 publications

An integrated approach to study the molecular aspects of regulatory peptides biological mechanism

An integrated approach to study the molecular aspects of regulatory peptides biological mechanism

Novel Insights into the Protective Properties of ACTH(4-7)PGP (Semax) Peptide at the Transcriptome Level Following Cerebral Ischaemia–Reperfusion in Rats

Synthetic corticotropins and the GABA‐receptor system: Direct and delayed effects

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