The protective effect of rutin and quercetin on 5-FU-induced hepatotoxicity in rats

Gelen, Volkan; Şengül, Emin; Gedikli, Semin; Atila, Gözde; Uslu, Hamit; Makav, Mustafa

doi:10.1016/j.apjtb.2017.06.013

Cited by 61 publications

(58 citation statements)

References 21 publications

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“…This protective effects of hesperidin on the liver as the result of its antioxidant properties .Treatment with rutin in preventive group liver showed congestion of the hepatoportal blood vessel and massive hepatocytes vacuolation with prepheral nucleous in therapeutic group. The protective effects of rutin indicated by study revealed that Quercitin and rutin might potentially be protective agents for 5-FU-induced liver toxicity [94] .…”

Section: Catalase Is a Hemoprotein And It Protects Cells From The Accmentioning

confidence: 99%

Assessment of antiproliferative activity of Capparis spinosa L extract against Ehrlich ascites carcinoma in Swiss albino mice

Mohammed

Gurigis

Gamal

2018

Biochemistry Letters

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Background: Medicinal plants have been known as one of the most important therapeutic agents for cancer treatment and prevention. Capparis spinosa L is a multipurpose plant that contains different bioactive phytochemicals including phenols and flavonoids.Aims: In the present study we explore the invivo antitumor activity of Capparis spinosa L extract and most potent constituents rutin and hesperidin against Ehrlich ascites carcinoma (EAC). Also study the side effect of treatments on different organs (liver, kidney and heart). Material and method: The antitumor effect was assessed by evaluating tumor volume , tumor cell count , survival time and increase in life span of EAC bearing mice. We assessed the effect of Capparis spinosa L extract , rutin and hesperidin on the level of malonaldialdhyde (MDA) and Catalase activity. Also, we estimated their effect on Alanine aminotransferase (ALT), Aspartate aminotransferase (AST) , Albumin , urea , creatinine ,Lactate dehydrogenase activity (LDH), Creatine Kinase-MB activity (CK-MB). Apoptosis was assessed by BCL2 and caspase3 activity. Results : The Capparis spinosa L extract , rutin and hesperidin showed significantly decreased (p<0.001) in the volume of the EAC and in the count of EAC cells and increase the life span of EAC bearing mice. The treatment with Capparis spinosa L extract , rutin and hesperidin showed significantly decreased (p<0.001) the lipid peroxidation marker (MDA) and recovered catalase activity towards normal as compared to positive control. We founded that Capparis spinosa L extract , rutin and hesperidin treatment induced apoptosis demonstrated by an increased in Caspase 3 activity and decreased in BCL2 .The treatment of Capparis spinosa L extract , rutin and hesperidin significantly reduced the elevated levels of ALT , AST ,LDH ,CK-MB,Urea and Creatinine in positive control as compared with negative control. Also, they showed protection for both liver and kidney histopathologically. Conclusions: The present study demonstrated that Capparis spinosa L extract , rutin and hesperidin have potent antitumor activity against Ehrlich ascites carcinoma. The anti-tumor mechanism may be mediated by preventing oxidative damage and induction of apoptosis.

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Section: Catalase Is a Hemoprotein And It Protects Cells From The Accmentioning

confidence: 99%

Assessment of antiproliferative activity of Capparis spinosa L extract against Ehrlich ascites carcinoma in Swiss albino mice

Mohammed

Gurigis

Gamal

2018

Biochemistry Letters

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“…Elevated concentration of these enzymes in the serum is an indication of loss of functional integrity of the hepatic membrane[ 39 , 40 ]. Elevated ALT level, especially, is a signal of hepatocellular necrosis and has been used as a surrogate marker of liver injury[ 41 ].…”

Section: Resultsmentioning

confidence: 99%

Amelioration of hepatotoxicity by biocleavable aminothiol chimeras of isoniazid: Design, synthesis, kinetics and pharmacological evaluation

Bhilare¹,

Dhaneshwar²,

Mahadik³

2018

WJH

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AIMTo overcome the hazardous effects on liver caused by long-term use of antitubercular agent isoniazid (INH) by developing a novel hepatoprotective prodrug strategy by conjugating INH with aminothiols as antioxidant promoities for probable synergistic effect.METHODSINH was conjugated with N-acetyl cysteine (NAC) and N-(2)-mercaptopropionyl glycine using the Schotten-Baumann reaction and with L-methionine using Boc-anhydride through a biocleavable amide linkage. Synthesized prodrugs were characterized by spectral analysis, and in vitro and in vivo release studies were carried out using HPLC. Their hepatoprotective potential was evaluated in male Wistar rats by performing liver function tests, measuring markers of oxidative stress and carrying out histopathology studies.RESULTSProdrugs were found to be stable in acidic (pH 1.2) and basic (pH 7.4) buffers and in rat stomach homogenates, whereas they were hydrolysed significantly (59.43%-94.93%) in intestinal homogenates over a period of 6 h. Upon oral administration of prodrug NI to rats, 52.4%-61.3% INH and 47.4%-56.8% of NAC were recovered in blood in 8-10 h. Urine and faeces samples pooled over a period of 24 h exhibited 1.3%-2.5% and 0.94%-0.9% of NAC, respectively, without any presence of intact NI or INH. Prodrugs were biologically evaluated for hepatoprotective activity. All the prodrugs were effective in abating oxidative stress and re-establishing the normal hepatic physiology. The effect of prodrug of INH with NAC in restoring the levels of the enzymes superoxide dismutase and glutathione peroxidase and abrogating liver damage was noteworthy especially.CONCLUSIONThe findings of this investigation demonstrated that the reported prodrugs can add safety and efficacy to future clinical protocols of tuberculosis treatment.

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“…Naringenin was reported to be the most potent in decreasing Bax overexpression (Ahmed et al 2019). The anti-apoptotic effect of rutin and quercetin was reported by Gelen et al (2017) through its effect on caspase-3 and Bcl-2 genes. The anti-apoptotic activity of gallic acid and coffeic acid appears to be mediated through a Bcl-2-independent mechanism (Khanduja et al 2006).…”

Section: Discussionmentioning

confidence: 99%

Innovative application of helium-neon laser: enhancing the germination of Adansonia digitata and evaluating the hepatoprotective activities in mice

Khamis

Hassan

Morsy

et al. 2020

Environ Sci Pollut Res

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The laser pretreatment of seed is drawing pronounced attention from the scientific community for its positive impact in boosting germination, seedling, and growth of plants. In this study, the laser pretreatment of Adansonia digitata (A. digitata) seeds was evaluated. Eight laser treatments were conducted at different powers, 10, 20, 40, and 80 mW, with the two-time interval for each power at 2 and 4 min. The outcomes indicated that the most efficient irradiation was 10 mW/2 min which induces the highest germination rate and polyphenolic contents for seeds. Based on these results, the animal experimental design was processed to assess the hepatoprotective activity of A. digitata extracts obtained through the optimum laser preillumination to enhance the resistance of liver damage in mice. The total phenol and flavonoid contents and the antioxidant properties of the methanolic extracts were estimated in vitro. The CCl4 was used to induce hepatotoxicity in mice. The animals were divided into five groups. The sera of the treated animals were used for the determination of transaminases, and the liver homogenates were used for the determination of antioxidant status, and further liver tissues were subjected to verify the anti-apoptotic effect of A. digitata methanolic extract. The in vivo results showed that the methanolic extract exposed to laser treatment at 10 mW/2 min provided better hepatoprotective capacity than the other treatments. Administration of A. digitata extract not only offered a significant decrease in liver enzyme activity but also markedly improved the antioxidant status and reduced the apoptotic progression induced by CCl4 toxicity in liver tissue.

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The protective effect of rutin and quercetin on 5-FU-induced hepatotoxicity in rats

Cited by 61 publications

References 21 publications

Assessment of antiproliferative activity of Capparis spinosa L extract against Ehrlich ascites carcinoma in Swiss albino mice

Assessment of antiproliferative activity of Capparis spinosa L extract against Ehrlich ascites carcinoma in Swiss albino mice

Amelioration of hepatotoxicity by biocleavable aminothiol chimeras of isoniazid: Design, synthesis, kinetics and pharmacological evaluation

Innovative application of helium-neon laser: enhancing the germination of Adansonia digitata and evaluating the hepatoprotective activities in mice

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