The Protective Effect of Melatonin vs. Vitamin E in the Ischemic/Reperfused Skeletal Muscle in the Adult Male Rat Model

Mohamed, Enas Ali Kamel

doi:10.4172/2157-7099.s3-005

Cited by 2 publications

(3 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Parallel regularly arranged muscle fibers, pale vesicular nuclei and rows of apparently normal mitochondria apart of some interstitial edema were recorded. These results were consistent with the findings of Mohamed, 2015 [20] who stated that vitamin E treated muscle sections showed relatively normal histological architecture with normal pattern, arrangement of the myofibrils and mitochondria.…”

Section: Discussionsupporting

confidence: 92%

A histological Study Comparing the Possible Protective Mechanisms of Oxytocin and Vitamin E on ischemia reperfusion injury in Rat skeletal muscle

Nada

El-Deeb

Aboulkhair

et al. 2018

Egyptian Journal of Histology

View full text Add to dashboard Cite

Background: Ischemia-reperfusion (IR) injury in skeletal muscle is a pathophysiology which affect quality of life. Aim of Work: To compare the possible protective mechanisms by which oxytocin and Vitamin E may exert on skeletal muscle IR injury in rat. Materials and Methods: 28 male rats were divided into: Control (Group A) of 10 rats and IR group (Group B) of 18 rats. Control group underwent the same surgical steps as IR group but without clamping the femoral artery followed by the injection of one of the following [saline (AI), oxytocin (AII) or vitamin E (AIII)]. In the IR group, lower limb ischemia was induced by clamping the femoral artery. After 4h of ischemia, the clamp was removed to allow reperfusion coinciding with the injection of one of the following [saline (BI), Oxytocin (0.5µg/kg) (BII) and Vitamin E (10mg/kg) (BIII)]. After 2 hours of reperfusion, Blood samples were drawn from the femoral vein to measure glutathione (GSH) and malondialdehyde (MDA). Gastrocnemius muscles were obtained for evaluation by light and electron microscopes. Cytochrome C, Myogenin immunohistochemistry and statistical analysis were applied. Results: A significant increase in GSH and significant decrease in MDA were reported in BII and BIII as compared to BI. Mean area % of cytochrome C in BII and BIII showed a significant decrease compared to BI. Mean number of myogenin immunopositive cells in BII was significantly higher compared to BI. Muscular damage was proved by light and electron microscopic findings in BI. While, BII and BIII encountered evident protection from muscular damage induced by IR injury. Conclusion: Oxytocin exerts weak protective antioxidant effect against IR injury. However, it activates stem cells to regenerate the damaged muscle. Vitamin E was better antioxidant which can react as early as 1 hour in the protection of the skeletal muscle against IR injury.

show abstract

Section: Discussionsupporting

confidence: 92%

A histological Study Comparing the Possible Protective Mechanisms of Oxytocin and Vitamin E on ischemia reperfusion injury in Rat skeletal muscle

Nada

El-Deeb

Aboulkhair

et al. 2018

Egyptian Journal of Histology

View full text Add to dashboard Cite

show abstract

“…The applied MLT was able to statistically significantly reduce CCl 4 -induced muscle tissue XO increase and these effects might be attributed to MLT direct inhibitory effects on XO (Kocic et al, 2017, Teixeira et al, 2003). The activity of XO is significantly increased by the generated ROS, thus MLT ability to reach to all tissue/cell compartments and to scavenge ROS (Mohamed, 2015) could be one of the mechanism involved in preventing XO increase as well.…”

Section: Discussionmentioning

confidence: 99%

“…In CCl 4 -induced tissue damage a decrease in cellular GSH content represents a logical occurrence, since this nonprotein thiol is involved in xenobiotics detoxification (Mohamed, 2015) including free radicals generated from CCl 4 . Although MLT was able to prevent CCl 4 -induced MDA formation and cellular enzymes leakage it was unable to preserve/increase cellular GSH (Table 1).…”

Section: Discussionmentioning

confidence: 99%

Effects of melatonin on oxidative stress parameters and pathohistological changes in rat skeletal muscle tissue following carbon tetrachloride application

Sokolović

Lilić²,

Milenković³

et al. 2018

Saudi Pharmaceutical Journal

View full text Add to dashboard Cite

Animal models demonstrating skeletal muscle (SM) disorders are rarely investigated, although these disorders accompany liver disorders and can occur during prolonged exercise/training. In cases of SM disorders exogenous antioxidants, such as melatonin, could help by generally improving tissues antioxidant capacities. We aimed to analyze the potential of melatonin in preventing biochemical and structural changes in rat biceps muscle (BM) occurring after an acute exposure to carbon tetrachloride (CCl4). Biceps muscles obtained from male Wistar rats belonging to different experimental groups were biochemically (determination of tissue MDA, total antioxidant capacity, GSH, CAT, SOD and GPx activities) and pathologically analyzed. Also, serum levels of potassium, LHD and CK were analyzed in all experimental animals. The obtained results were statically compared with those from vehicle-treated control group. The applied melatonin prevented potassium and intracellular enzyme leakage (CK and LDH) that was induced by CCl4, as well as an increase in tissue MDA. From a panel of determined oxidative stress parameters melatonin was able to statistically significantly prevent changes in total antioxidative capacity and in CAT, SOD and GPx activities induced by CCl4. Microscopic analysis of BM from the animals exposed to CCl4 revealed significant muscle fiber disorganization and massive inflammatory cell infiltration. All these changes were significantly ameliorated in the group that received melatonin prior to CCl4. Changes in serum and tissue biochemical parameters accompanied the observed pathological changes, which demonstrated a significant influence of melatonin in preventing skeletal muscle damage induced by CCl4.

show abstract

The Protective Effect of Melatonin vs. Vitamin E in the Ischemic/Reperfused Skeletal Muscle in the Adult Male Rat Model

Cited by 2 publications

References 19 publications

A histological Study Comparing the Possible Protective Mechanisms of Oxytocin and Vitamin E on ischemia reperfusion injury in Rat skeletal muscle

A histological Study Comparing the Possible Protective Mechanisms of Oxytocin and Vitamin E on ischemia reperfusion injury in Rat skeletal muscle

Effects of melatonin on oxidative stress parameters and pathohistological changes in rat skeletal muscle tissue following carbon tetrachloride application

Contact Info

Product

Resources

About