The protective effect of adenosine triphosphate-MgCl2 on ischemia-reperfusion lung injury is leukocyte dependent

Purinergic Signaling and Blood Vessels in Health and Disease

“…Lung. Ischemia-reperfusion lung injury was attenuated by ATP-MgCl 2 in rats, although its effect was in part mediated by its breakdown product, adenosine, via A 2 receptors, probably on leukocytes, acting to prevent the production of damaging O 2 -derived free radicals (Hsu et al, 1994;Hirata et al, 2001;Chen et al, 2003). A 2A receptor agonists attenuated cardiac dysfunction arising from pulmonary ischemia-reperfusion injury (Reece et al, 2005;Ellman et al, 2008;Gazoni et al, 2008;Sharma et al, 2009).…”

Purinergic Signaling in the Airways

“…ATP-dependent calcium signaling during ventilator-induced lung injury is amplified by hypercapnia (Briva et al, 2011). The protective effect of ATP-MgCl 2 in ischemia-reperfusion lung injury seems to require the presence of leukocytes (Chen et al, 2003).…”

Biomedical vignette

“…A previous study [14] indirectly suggested that Mg 2+dependent ecto-ATPases on the surface of leukocTtes are responsible for the hydrolysis of ATP-MgC12 to adenosine, which then contributes to the protective effect of ATP-MgCI2. then et al [7], in a buffer-perfused isolated rat lung model, found that ischemia-reperfusion increases capillary permeability, lung weight gain, wet lung/body weight ratio, lung lavaged protein concen-tration and pulmonary arterial pressure. These increases were significantly attenuated when Ieukocytes were added to the ATP-MgCl 2 pretreatment.…”