The protection of MSCs from apoptosis in nerve regeneration by TGFβ1 through reducing inflammation and promoting VEGF-dependent angiogenesis

Luo, Hongyan; Zhang, Yongjie; Zhang, Ziqiang; Jin, Yan

doi:10.1016/j.biomaterials.2012.02.042

Cited by 66 publications

(62 citation statements)

References 40 publications

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“…According to the results obtained, all three cell types secreted growth factors, which facilitate angiogenesis, such as angiogenin, 32 angiopoietin-1, 33 endostatin/collagen XVIII, 34 transforming growth factor- β 1 (TGF β 1) 35 and vascular endothelial growth factor (VEGF) 19, 36 (Figure 7b i). The Fibroblast growth factor-7 (FGF-7), 37 Serpin E1, Serpin F1 38 and urinary plasminogen activator (uPA) 39 proteins, responsible for the tissue repair and regeneration, were also detected in CM derived from AF-, DAF- and TRAF-MSCs (Figure 7b ii).…”

Section: Resultsmentioning

confidence: 95%

AF-MSCs fate can be regulated by culture conditions

et al. 2013

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Human mesenchymal stem cells (hMSCs) represent a population of multipotent adherent cells able to differentiate into many lineages. In our previous studies, we isolated and expanded fetal MSCs from second-trimester amniotic fluid (AF) and characterized them based on their phenotype, pluripotency and proteomic profile. In the present study, we investigated the plasticity of these cells based on their differentiation, dedifferentiation and transdifferentiation potential in vitro. To this end, adipocyte-like cells (AL cells) derived from AF-MSCs can regain, under certain culture conditions, a more primitive phenotype through the process of dedifferentiation. Dedifferentiated AL cells derived from AF-MSCs (DAF-MSCs), gradually lost the expression of adipogenic markers and obtained similar morphology and differentiation potential to AF-MSCs, together with regaining the pluripotency marker expression. Moreover, a comparative proteomic analysis of AF-MSCs, AL cells and DAF-MSCs revealed 31 differentially expressed proteins among the three cell populations. Proteins, such as vimentin, galectin-1 and prohibitin that have a significant role in stem cell regulatory mechanisms, were expressed in higher levels in AF-MSCs and DAF-MSCs compared with AL cells. We next investigated whether AL cells could transdifferentiate into hepatocyte-like cells (HL cells) directly or through a dedifferentiation step. AL cells were cultured in hepatogenic medium and 4 days later they obtained a phenotype similar to AF-MSCs, and were termed as transdifferentiated AF-MSCs (TRAF-MSCs). This finding, together with the increase in pluripotency marker expression, indicated the adaption of a more primitive phenotype before transdifferentiation. Additionally, we observed that AF-, DAF- and TRAF-MSCs displayed similar clonogenic potential, secretome and proteome profile. Considering the easy access to this fetal cell source, the plasticity of AF-MSCs and their potential to dedifferentiate and transdifferentiate, AF may provide a valuable tool for cell therapy and tissue engineering applications.

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Section: Resultsmentioning

confidence: 95%

AF-MSCs fate can be regulated by culture conditions

et al. 2013

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“…It has been suggested that participation in host immune responses is only one of the many functions of lymphocytes, since lymphoid cells also participate in a number of physiological processes of homeostasis [44]. While a lot of work has been done on the role of various growth factors and cytokines produced by mesenchymal cells on the cell cycle and death in vitro [45-58], still little is known about the interactions between mesenchymal and tissue-specific cells in vivo . The role of immune system components in the regulation of tissue physiology and pathology should also be viewed more widely, along with resident mesenchymal cells, such as fibroblast-derived vascular pericytes, and autonomic neural signals.…”

Section: Immune System and Tissue Homeostasis (Tissue Immune Physimentioning

confidence: 99%

Immunoregulation of follicular renewal, selection, POF, and menopause in vivo, vs. neo-oogenesis in vitro, POF and ovarian infertility treatment, and a clinical trial

Bukovský

Caudle

2012

Reprod Biol Endocrinol

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The immune system plays an important role in the regulation of tissue homeostasis ("tissue immune physiology"). Function of distinct tissues during adulthood, including the ovary, requires (1) Renewal from stem cells, (2) Preservation of tissue-specific cells in a proper differentiated state, which differs among distinct tissues, and (3) Regulation of tissue quantity. Such morphostasis can be executed by the tissue control system, consisting of immune system-related components, vascular pericytes, and autonomic innervation. Morphostasis is established epigenetically, during morphogenetic (developmental) immune adaptation, i.e., during the critical developmental period. Subsequently, the tissues are maintained in a state of differentiation reached during the adaptation by a “stop effect” of resident and self renewing monocyte-derived cells. The later normal tissue is programmed to emerge (e.g., late emergence of ovarian granulosa cells), the earlier its function ceases. Alteration of certain tissue differentiation during the critical developmental period causes persistent alteration of that tissue function, including premature ovarian failure (POF) and primary amenorrhea. In fetal and adult human ovaries the ovarian surface epithelium cells called ovarian stem cells (OSC) are bipotent stem cells for the formation of ovarian germ and granulosa cells. Recently termed oogonial stem cells are, in reality, not stem but already germ cells which have the ability to divide. Immune system-related cells and molecules accompany asymmetric division of OSC resulting in the emergence of secondary germ cells, symmetric division, and migration of secondary germ cells, formation of new granulosa cells and fetal and adult primordial follicles (follicular renewal), and selection and growth of primary/preantral, and dominant follicles. The number of selected follicles during each ovarian cycle is determined by autonomic innervation. Morphostasis is altered with advancing age, due to degenerative changes of the immune system. This causes cessation of oocyte and follicular renewal at 38 +/-2 years of age due to the lack of formation of new granulosa cells. Oocytes in primordial follicles persisting after the end of the prime reproductive period accumulate genetic alterations resulting in an exponentially growing incidence of fetal trisomies and other genetic abnormalities with advanced maternal age. The secondary germ cells also develop in the OSC cultures derived from POF and aging ovaries. In vitro conditions are free of immune mechanisms, which prevent neo-oogenesis in vivo. Such germ cells are capable of differentiating in vitro into functional oocytes. This may provide fresh oocytes and genetically related children to women lacking the ability to produce their own follicular oocytes. Further study of "immune physiology" may help us to better understand ovarian physiology and pathology, including ovarian infertility caused by POF or by a lack of ovarian follicles with functional oocytes in aging ovaries. The observations indicating...

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“…due the cells between fingers occured apoptosis, we can form five fingers per hand. Apoptosis also can enhance embryonic stem cell survival during stress by increased expression Bcl-2 protein which significantly weaken the apoptosis and help colony formation [16];Apoptosis also take part in some tissues regeneration and homeostasis [17,18]; In addition to the function that we have described above, apoptosis can still anti-inflammation and hamper pathogens persistence infection, especially interfering the intracellular parasites diffusion; In conclusion, apoptosis are good for people's healthy in variety aspects, and apoptosis become research areas hotspots at present. Summarizing the currently researcher's data, we found that many ligands can trigger or inhibit apoptosis.…”

Section: Trigger Apoptosis Ligands and Cell Environment Materialsmentioning

confidence: 99%