The protease-based compensatory mechanism to minimize the effect of dietary Soybean Trypsin Inhibitor in Litopenaeus vannamei

Rojo‐Arreola, Liliana; Choquet, Cyril; Córdova-Murueta, Julio H.; García‐Carreño, Fernando

doi:10.1016/j.aquaculture.2018.10.002

Cited by 11 publications

(7 citation statements)

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“…The main P . vannamei metallopeptidase belongs to the astacin family and the transcript is found primarily in the digestive system [ 16 , 63 ]. The expression of this enzyme was detected from late mysis stage and increasing at decapodid stages where a significant increment of activity is also observed ( Fig 4D ), such activity peak has been reported before [ 16 ].…”

Section: Discussionmentioning

confidence: 99%

“…Metallo-peptidase activity was also assessed (Fig 4C), larval zinc metallopeptidase expression and activity had been reported mainly in the infective stages of helminths and insects [60,61], but also as fat-body remodeling enzymes in insect larval stages [62]. The main P. vannamei metallopeptidase belongs to the astacin family and the transcript is found primarily in the digestive system [16,63]. The expression of this enzyme was detected from late mysis stage and increasing at decapodid stages where a significant increment of activity is also observed (Fig 4D), such activity peak has been reported before [16].…”

Section: Plos Onementioning

confidence: 99%

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Proteolytic profile of larval developmental stages of Penaeus vannamei: An activity and mRNA expression approach

et al. 2020

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In arthropods, the cleavage of specific proteins by peptidases has pivotal roles in multiple physiological processes including oogenesis, immunity, nutrition, and parasitic infection. These enzymes are also key players in the larval development, and well-described triggers of molting and metamorphosis. In this work the peptidase complement throughout the larvae development of Penaeus vannamei was quantified at the transcript and activity level using qPCR and fluorogenic substrates designed to be hydrolyzed by class-specific peptidases respectively, providing a detailed identification of the proteolytic repertoire in P. vannamei larvae. Significant changes in the peptidase activity profile were observed. During the lecithotrophic naupliar instars, the dominant peptidase activity and expression derive from cysteine peptidases, suggesting that enzymes of this class hydrolyze the protein components of yolk as the primary amino acid source. At the first feeding instar, zoea, dominant serine peptidase activity was found where trypsin activity is particularly high, supporting previous observations that during zoea the breakdown of food protein is primarily enzymatic. At decapodid stages the peptidase expression and activity is more diverse indicating that a multienzyme network achieves food digestion. Our results suggest that proteolytic enzymes fulfill specific functions during P. vannamei larval development.

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Section: Discussionmentioning

confidence: 99%

Section: Plos Onementioning

confidence: 99%

Proteolytic profile of larval developmental stages of Penaeus vannamei: An activity and mRNA expression approach

et al. 2020

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“…has the function to the inhibitor of trypsin and chymotrypsin, this highly stable proteinaceous inhibitor can resist the heat treatment during the drying process feed fabrication. Therefore the molecule is often present in its active form in commercial shrimp feed (Rojo-Arreola et al, 2019;Lemos et al, 2000 andMaytorena-Verdugo et al, 2017). Soybean Trypsin Inhibitor (S.B.T.I.)…”

Section: Introductionmentioning

confidence: 99%

“…Soybean Trypsin Inhibitor (S.B.T.I.) binds both trypsins and chymotrypsins of Litopenaeus vannamei with 80-90% inhibition at 0.25 mM (Rojo-Arreola et al, 2019;Hernandez-Cortes et al, 1997;Sainz et al, 2004;Maytorena-Verdugo et al, 2017). However, shrimp respond to phenotypic plasticity in the digestive system of L. vannamei as an adaptive response to compensate the protein digestion capacity when some of the peptidase activities are reduced by the presence of protease inhibitors (Rojo-Arreola et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

“…binds both trypsins and chymotrypsins of Litopenaeus vannamei with 80-90% inhibition at 0.25 mM (Rojo-Arreola et al, 2019;Hernandez-Cortes et al, 1997;Sainz et al, 2004;Maytorena-Verdugo et al, 2017). However, shrimp respond to phenotypic plasticity in the digestive system of L. vannamei as an adaptive response to compensate the protein digestion capacity when some of the peptidase activities are reduced by the presence of protease inhibitors (Rojo-Arreola et al, 2019). Therefore, this study investigated the feasibility and optimal condition of crude aqueous extract from pineapple byproducts action to improve the protein digestibility and to be a basic data for considering to modify soybean meal to be a perfect protein source.…”

Section: Introductionmentioning

confidence: 99%

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THE EVALUATION OF PROTEIN DIGESTIBILITY, SAPONIN AND TRYPSIN INHIBITOR CONTENT IN PACIFIC WHITE SHRIMP (Litopenaeus vanamei) FEED, DIGESTED WITH BROMELAIN CRUDE EXTRACT FROM PINEAPPLE WASTE

Klahan¹,

Bundit²,

Wiboonsirikul³

et al. 2022

Proceedings of International Conference on Fisheries and Aquaculture

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The present study aimed at investigating the effect of varying concentrations of bromelain crude extract from pineapple on percentage of protein digestibility and amount of saponin, and trypsin inhibitor in pacific white shrimp feed. The bromelain was extracted from the crown and peel of pineapple (Bhattavia strain) at dark green ripening stages. The trial was divided into two experiments; the first experiment determined the optimal condition for in vitro protein digestibility of pacific white shrimp feed which contains 38% of crude protein. It was digested by bromelain crude extract at different pH (6 -9), hydrolysis time (5, 10, and 30 min), and temperature (25 and 30 °C). The second experiment investigated the saponin and trypsin inhibitor (TI) in white shrimp feed after being digested with different concentrations of bromelain crude extract at 0, 90, 170, and 250 ppt for 5, 10, and 30 min at 30 °C. The results presented that the optimal condition for protein digestion with bromelain was pH 6 at 25 °C for 5 and 30 min, the percentage of protein digestion was 63.15 and 70.66% (P<0.05). Besides, saponin content in the diet was varied after the bromelain levels and hydrolysis time, the highest level of saponin was found in digested diet with bromelain at 170 and 250 ppt for 30 min (1.84 and 1.88 mg/g feed) and the lowest saponin at 90 and 170 ppt for 5 min. (0.94 and 0.99 mg/g feed) (P<0.05). The TI content in the diet was varied inversely with the levels of bromelain at 5 minute length which the lowest level of TI was shown in digested feed with bromelain at 250 ppt (0.008 mg/g feed) (P<0.05) whereas TI was varied with bromelain at 10 and 30minute lenght which the lowest level was shown at 0 ppt (0.0031 and 0.007mg/g feed) (P<0.05). This study showed that the optimal condition for protein digestion with bromelain was pH 6 at 25°C for 5 and 30 min, and the suitable level of bromelain and hydrolysis time to inactivate the saponin and trypsin inhibitor in shrimp feed were 250 ppt at 5 min.

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Understanding the Digestive Peptidases from Crustaceans: from Their Biochemical Basis and Classical Perspective to the Biotechnological Approach

Muhlia‐Almazán

Fernández-Gimenez

2022

Mar Biotechnol

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The protease-based compensatory mechanism to minimize the effect of dietary Soybean Trypsin Inhibitor in Litopenaeus vannamei

Cited by 11 publications

References 33 publications

Proteolytic profile of larval developmental stages of Penaeus vannamei: An activity and mRNA expression approach

Proteolytic profile of larval developmental stages of Penaeus vannamei: An activity and mRNA expression approach

THE EVALUATION OF PROTEIN DIGESTIBILITY, SAPONIN AND TRYPSIN INHIBITOR CONTENT IN PACIFIC WHITE SHRIMP (Litopenaeus vanamei) FEED, DIGESTED WITH BROMELAIN CRUDE EXTRACT FROM PINEAPPLE WASTE

Understanding the Digestive Peptidases from Crustaceans: from Their Biochemical Basis and Classical Perspective to the Biotechnological Approach

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