The Prostaglandin Synthesis in Carp Thrombocyte

Kayama, Mitsu; Sadõ, Tetsuya; Iijima, Noriaki; Asada, Toshio; Igarashi, Michiko; Shiba, Tadaaki; Yamaguchi, Ryosuke; Hirai, Toshio

doi:10.2331/suisan.51.1911

Cited by 23 publications

(3 citation statements)

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“…The composition of PI, particularly the high level 20:4(n-6) is consistent with a role as a precursor pool for eicosanoid synthesis in normally (n-3)-rich fish tissues as postulated previously (Bell et al 1983;Tocher and Sargent 1984). Metabolic studies showed that various trout tissues and thrombocytes produced cyclooxygenase products from endogenously ( Christ and Van Dorp 1972) and exogenously (Kayama et al 1985) added 20:4(n-6). Similarly 20:4(n-6) was metabolised via lipoxygenase to 12-hydroxyeicosatetraenoic acid in trout skin (German et al 1985) and to mono-(mainly 12-hydroxy) ) and trihydroxy derivatives in trout gill .…”

Section: Discussionmentioning

confidence: 63%

Incorporation and metabolism of (n-3) and (n-6) polyunsaturated fatty acids in phospholipid classes in cultured rainbow trout (Salmo gairdneri) cells

Tocher

1990

Fish Physiol Biochem

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The incorporation and metabolism of various (n-3) and (n-6) polyunsaturated fatty acids supplemented to the culture medium was investigated in the rainbow trout cell line, RTG-2. The distribution, and the occurrence and relative extent of further desaturation and elongation of the incorporated acids was determined in individual phospholipid classes by analysis of the fatty acid compositions. RTG-2 cells exhibited Δ6 and Δ5 desaturase activities whereas Δ4 desaturase activity was almost totally absent. The percentage of precursor acids was greatest in the phosphatidic acid/cardiolipin fraction (PA/CL), suggesting a role for possibly PA in the initial incorporation of these acids into the phospholipid pool. The compositional data indicated that individual intermediates and products of the desaturation pathways were associated with specific phospholipid classes probably via mechanisms depending upon the specificities of the acylating enzymes. The composition of phosphatidylinositol (PI) and the tightly controlled mechanisms for generating/maintaining it are consistent with a role for this phospholipid in providing precursor fatty acid for eicosanoid synthesis.

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Section: Discussionmentioning

confidence: 63%

Incorporation and metabolism of (n-3) and (n-6) polyunsaturated fatty acids in phospholipid classes in cultured rainbow trout (Salmo gairdneri) cells

Tocher

1990

Fish Physiol Biochem

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“…Thromboxane B, is a molecule with little biological activity, but its unstable precursor, TXA,, causes both the constriction of blood vessels and the aggregation of platelets to form a thrombus (Taussig, 1984). In the carp, Cyprinus carpio, analysis of eicosanoid products of purified thrombocytes failed to find any TXB,, though the presence of PGE,, PGD, and PGF,, were noted (Kayama et al, 1985). Thus, it would be interesting to study thrombocyte behaviour during aggregation in fish such as carp, in which TXB, is lacking.…”

Section: Discussionmentioning

confidence: 99%

Preliminary studies on eicosanoid production by fish leucocytes, using GC‐mass spectrometry

Rowley¹,

Barrow²,

Hunt³

1987

Journal of Fish Biology

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Culture supernatants from dogfish leucocytes, exposed to the CaZ+ ionophore A23187, were analyzed for eicosanoid production by gas chromatography-electron capture mass spectrometry. Consistently high levels of the prostaglandins (PG) D,, F,, and E, were produced, while thromboxane B, and leukotriene B, were found in smaller amounts. No 6-0x0 PGF,,, a degradation product of prostacyclin, or 13, l4-dihydro-l5-oxo-PGF2,, a metabolite of PGE, and PGF,,, were detected. The results are compared with similar experiments using mammalian leucocytes.

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“…Είναι γενικά αποδεκτό ότι σε περιπτώσεις καταστροφής της δομής των κυττάρων, η βιοσύνθεση των εικοσανοϊκών ενώσεων εντείνεται ιδιαίτερα (Jobling, 1995) Πρέπει τέλος να τονιστεί ότι η ικανότητα των ιχθύων να συνθέτουν εικοσανοϊκά από τα πρόδρομα λιπαρά οξέα που προαναφέρθηκαν, έχει διαπιστωθεί τόσο σε είδη ιχθύων γλυκού νερού (π.χ. κυπρίνος Cyprinus ca/777'o- Kayama et al, 1985, σαλμερίνος Salvelinus fontinalis-Hsu and Goetz, 1993, πέστροφα Oncorhynchus mykiss-Rowley et al, 1994 όσο και σε είδη ιχθύων θαλασσινού νερού (π.χ. κόκκινη τσιπούρα Pagrus wq/or- Iijima et al, 1996, πισσί Scophthalmus maximus-lochex et al, 1996, λαβράκι Dicentrarchus labrax-Farndale et al, 1999 (Mustafa andJensen, 1992, Heath, 1995 (Sargent et al, 1989).…”

Section: ιοντικη και ωσμωτικη ρυθμισηunclassified

Διερεύνηση Της Επιδράσεως Των Αιωρούμενων Σωματιδίων Του Νερού Εκτροφής Στη Φυσιολογία Εκτρεφόμενων Ιχθύων

Karakatsouli¹,

Καρακατσούλη²

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Εικόνα 8. Εκατοστιαία διαφορά (%) των επιπέδων των λιπαρών οξέων του περισπλαχνικού λίπους των ιχθυοπληθυσμών του κυπρίνου "λέπια", οι οποίοι υποβλήθηκαν στις αυξημένες συγκεντρώσεις ΟΑΣ (ppm) επί 3 μήνες, από τα αντίστοιχα του πληθυσμού μάρτυρα

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The Prostaglandin Synthesis in Carp Thrombocyte

Cited by 23 publications

References 0 publications

Incorporation and metabolism of (n-3) and (n-6) polyunsaturated fatty acids in phospholipid classes in cultured rainbow trout (Salmo gairdneri) cells

Incorporation and metabolism of (n-3) and (n-6) polyunsaturated fatty acids in phospholipid classes in cultured rainbow trout (Salmo gairdneri) cells

Preliminary studies on eicosanoid production by fish leucocytes, using GC‐mass spectrometry

Διερεύνηση Της Επιδράσεως Των Αιωρούμενων Σωματιδίων Του Νερού Εκτροφής Στη Φυσιολογία Εκτρεφόμενων Ιχθύων

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