The Prospects of Using Structural Phase Analysis of Microcalcifications in Breast Cancer Diagnostics

Piddubnyi, Artem; Kolomiiets, Olena; Danilchenko, Sergey; Stepanenko, Andriy; Moskalenko, Yuliia; Moskalenko, Roman

doi:10.3390/diagnostics13040737

Cited by 2 publications

(2 citation statements)

References 31 publications

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“…In general, the procedure of immunohistochemical staining of the tissue was carried out according to the method previously described in our works [5]. We used primary antibodies against CD163 (clone EP 324, Master Diagnostica, ready to use), and CD68 (clone Ab-3, Thermo Fisher Scientific, at a dilution of 1:200).…”

Section: Immunohistochemistry Of Breast Cancer Tissuementioning

confidence: 99%

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Immunohistochemical Study of M1 and M2 Macrophages in Breast Cancer With Microcalcifications

Kolomiiets

Moskalenko

2023

EUMJ

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Introduction. Breast cancer (BC) is a significant medical and social problem, as it is the leading cause of cancer-related mortality in women worldwide. Microcalcifications in the breast tissue are essential in developing the pathological process and affect the prognosis and metastasis. The tumor microenvironment consists of cancer cells and stromal cells such as fibroblasts, endothelial cells, pericytes, and immune cells, including M1 and M2 macrophages. The work aims to study the influence of microcalcifications on the polarization of macrophages in the tumor microenvironment of BC. Materials and methods. The study was conducted on 60 samples of BC, divided into 30 samples of BC with microcalcifications (group I) and a control group of 30 samples of BC without calcifications (group II). All microcalcifications met the criterion of size up to ≤1 mm. To study the pathohistological changes, BC's tissue was analyzed using macroscopic description, histology, and immunohistochemical study with antibodies against CD68 and CD163. Results. According to the results of an immunohistochemical study, it was found that the expression of CD68-positive macrophages of the M1 type is significantly higher in the tissue of samples of BC with microcalcifications, compared to samples of the control group (60.85 ± 2.71 cells in the field of view vs. 51.14 ± 2.89, p < 0.05). On the other hand, it was established that the average value of CD163 expression in group I was 53.21 ± 3.05 against 65.57 ± 3.75 (p ˂ 0.05) cells in the group of BC without calcification. Nevertheless, the localization of M1 and M2 macrophages in the tumor had standard features and did not differ. A possible mechanism of the effect of microcalcifications on the polarization of macrophages is their support of chronic inflammation in cancer tissues and, thus, the development of pro-inflammatory M1 phenotype in macrophages. Further studies are needed to obtain more unambiguous conclusions.

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Section: Immunohistochemistry Of Breast Cancer Tissuementioning

confidence: 99%

“…Microcalcifications in the breast tissue play an essential role in developing the pathological process and affect the prognosis and metastasis. Their presence in the tissue of BC is a criterion for determining the stage of the disease and early and differential diagnosis of BC [3,4,5].…”

Section: Introduction / вступmentioning

confidence: 99%

Immunohistochemical Study of M1 and M2 Macrophages in Breast Cancer With Microcalcifications

Kolomiiets

Moskalenko

2023

EUMJ

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A Comprehensive Study of Meningioma Biomineralization: Morphological, Crystallographic, and Immunohistochemical Aspects

Denysenko,

Danilchenko,

Stepanenko

et al. 2024

Microscopy and Microanalysis

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Biomineralization of brain tissues occurs both in normal and pathological conditions. Dura mater biomineralization is widespread and occurs in 1–72% of cases, depending on the patient's age and research method. The amount of biomineral deposits under the conditions of tumor growth in the meninges only increases, reaching 100% in the case of psammomatous meningiomas. Since calcifications are often found in the meninges, the problem of differential diagnosis with calcified meningiomas arises. A total of 30 samples of meningiomas with signs of biomineralization—dense structure, characteristic crunch, psammoma bodies (group I) and 30 samples of meningiomas without any signs of biomineralization were examined as controls (group II). To detect pathological biomineralization, the meningioma tissue was studied using the methods of macroscopic description, histology, histochemistry, and immunohistochemistry, scanning electron microscopy with microanalysis, and transmission electron microscopy. A significantly higher level of caspase3 and features of the expression of osteoblastic markers (a lower level of OPG expression and a higher level of the presence of RANKL in group I, the absence of fluctuations in the expression of SPARC) may indicate a dystrophic type of development of biomineral deposits in meningiomas.

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The Prospects of Using Structural Phase Analysis of Microcalcifications in Breast Cancer Diagnostics

Cited by 2 publications

References 31 publications

Immunohistochemical Study of M1 and M2 Macrophages in Breast Cancer With Microcalcifications

Immunohistochemical Study of M1 and M2 Macrophages in Breast Cancer With Microcalcifications

A Comprehensive Study of Meningioma Biomineralization: Morphological, Crystallographic, and Immunohistochemical Aspects

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