The Properties of Alamethicin Incorporated Into Planar Lipid Bilayers Under the Influence of Microgravity

Klinke, Nicole; Goldermann, M.; Hanke, Wolfgang

doi:10.1016/s0094-5765(00)00115-6

Cited by 16 publications

(6 citation statements)

References 3 publications

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“…Two models for ion channels have been used to show the clear gravity dependence of the open-state probability of ion channels (Goldermann and Hanke 2001 ; Klinke et al 2000 ), but there is no single channel data for real (neuronal) ion channels. Although the whole cell recordings from several research groups indicate that there is a gravity dependence of ion channels (Goldermann and Hanke 2001 ; Klinke et al 2000 ; Schaffhauser et al 2011 ; Richard et al 2012 ), it is still unclear from the literature if all ion channel families, e.g., the relevant ion channel families for AP generation, react similar to changes in gravity. For the proposed model, this is assumed, but it still has to be investigated much more systematically.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Gravity and neuronal adaptation, in vitro and in vivo—from neuronal cells up to neuromuscular responses: a first model

Kohn

Ritzmann

2017

Eur Biophys J

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For decades it has been shown that acute changes in gravity have an effect on neuronal systems of human and animals on different levels, from the molecular level to the whole nervous system. The functional properties and gravity-dependent adaptations of these system levels have been investigated with no or barely any interconnection. This review summarizes the gravity-dependent adaptation processes in human and animal organisms from the in vitro cellular level with its biophysical properties to the in vivo motor responses and underlying sensorimotor functions of human subjects. Subsequently, a first model for short-term adaptation of neuronal transmission is presented and discussed for the first time, which integrates the responses of the different levels of organization to changes in gravity.

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Section: Discussionmentioning

confidence: 99%

“…A second model system used is alamethicin, a pore-forming peptide from Trichoderma viride . Similar to the E. coli porins, the activity of alamethicin is increased towards higher gravity (>1 g) and is decreased towards microgravity (Klinke et al 2000 ; Wiedemann et al 2003 ).…”

Section: Subcellular Parametersmentioning

confidence: 99%

Gravity and neuronal adaptation, in vitro and in vivo—from neuronal cells up to neuromuscular responses: a first model

Kohn

Ritzmann

2017

Eur Biophys J

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“…Similar findings have been made with alamethicin, a pore-forming peptide from Trichoderma viride. In microgravity, the activity of alamethicin is decreased, whereas in hypergravity, it is increased [25,26].…”

Section: Ion Channel Parametersmentioning

confidence: 96%

“…The pore activity of alamethicin and the open-state probability of ion channels is also gravitydependent [24,25]. In microgravity, the open-state probability decreases, whereas in hypergravity, it increases.…”

Section: Molecular Levelmentioning

confidence: 99%

The Effect of Gravity on the Nervous System

Kohn¹,

Koch²,

Ritzmann³

2018

Into Space - A Journey of How Humans Adapt and Live in Microgravity

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Gravity affects the nervous system of living organisms. This book chapter reviews historical and recent findings on how changes in gravity affect cellular and subcellular parameters of human and animal cells as well as the timing and shaping of complex sensorimotor responses. With an emphasize on weightlessness, partial, and hypergravity conditions, the gravity dependencies of living organisms have been manifested on different levels of organization, ranging from changes in biophysical properties of single cells to the intact nervous system. An effort has been made to integrate the various findings into a consistent model for a better understanding of how the components of the nervous system interact as a response to acute and long-term gravitational variation. Especially with planned long-term manned missions to Mars and beyond, knowledge about the impact of increased and decreased gravity on the nervous system is essential for the physical and cognitive preparation to assure the success of space missions and human survival in space.

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“…isms [1] [2] to cells [3], to membranes [2] and even down to the function of single proteins, especially ionchannels [4]- [6]. Nevertheless, related to the question how membranes and single protein function are affected by gravity there are still a variety of problems existing in interpretation, which concern the basic underlying mechanisms.…”

Section: Introductionmentioning

confidence: 99%

Conductance and Capacity of Plain Lipid Membranes under Conditions of Variable Gravity

Sieber¹,

Kaltenbach²,

Hanke³

et al. 2016

JBiSE

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Biological cell membranes are complex structures containing mainly lipids and proteins. Functional aspects of such membranes are usually attributed to membrane integral proteins. However, it is well established that parameters of the lipid matrix are modifying the function of proteins. Additionally, electrical capacity and conductance of the plain lipid matrix of membranes are contributing directly to cellular functions as there is, for example, the propagation of action potentials. Accordingly the dependence of these parameters on changes of gravity might be important in the field of life sciences under space conditions. In this study consequently we have performed experiments in parabolic flight campaigns utilizing the patch-clamp technology to investigate conductance and capacity of plain lipid vesicle membranes under conditions of changing gravity. Both capacity and conductance were found to be gravity dependent. The changes in capacity could be contributed to changes in membrane geometry. Significant permeability in plain lipid membranes could be only observed at high potentials, where spontaneous current fluctuations occurred. The probability of these fluctuations was gravity dependent.

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The Properties of Alamethicin Incorporated Into Planar Lipid Bilayers Under the Influence of Microgravity

Cited by 16 publications

References 3 publications

Gravity and neuronal adaptation, in vitro and in vivo—from neuronal cells up to neuromuscular responses: a first model

Gravity and neuronal adaptation, in vitro and in vivo—from neuronal cells up to neuromuscular responses: a first model

The Effect of Gravity on the Nervous System

Conductance and Capacity of Plain Lipid Membranes under Conditions of Variable Gravity

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