We present spatio-temporal characteristics of spreading depolarizations (SD) in two experimental systems: retracting SD wave segments observed with intrinsic optical signals in chicken retina, and spontaneously occurring re-entrant SD waves that repeatedly spread across gyrencephalic feline cortex observed by laser speckle flowmetry. A mathematical framework of reaction-diffusion systems with augmented transmission capabilities is developed to explain the emergence and transitions between these patterns. Our prediction is that the observed patterns are reaction-diffusion patterns controlled and modulated by weak nonlocal coupling. The described spatio-temporal characteristics of SD are of important clinical relevance under conditions of migraine and stroke. In stroke, the emergence of re-entrant SD waves is believed to worsen outcome. In migraine, retracting SD wave segments cause neurological symptoms and transitions to stationary SD wave patterns may cause persistent symptoms without evidence from noninvasive imaging of infarction.
Biological membranes are preferentially composed of lipids and proteins, and it is assumed that mainly the proteins are responsible for their functional properties. Nevertheless, during the last years, the contribution of the plain lipid matrix and its physico-chemical parameters to membrane functionality has been shown to be of high relevance. This is also correct for the gravity dependence of cells and organisms which is well accepted since long for a wide range of biological systems. Thus, the question must be asked, whether, and how far plain lipid membranes are affected by gravity directly. In this study we show that the fluidity (viscosity) of plain lipid membranes, as well as that of cell membranes, is gravity dependent, using a multipurpose 96-well plate reader in the fluorescence polarization anisotropy mode in a parabolic flight mission. Plain lipid vesicles and cells from a human cancer cell line have been used in these experiments. Necessarily, membrane-integrated proteins should be affected by this in their function. As a consequence any living cell will be able to sense at least basically gravity.
Utilizing the intrinsic optical signal (IOS) of retinal Spreading Depression (rSD) waves and the concomitant transparency changes of the tissue, we show that nicotine, in an in vivo near tissue preparation, has neuroprotective effects against the excitotoxic cell death, mediated through the activation of glutamate-receptors. For this study the retinal tissue was treated with NMDA, an agonist for the NMDA-glutamate-receptor, being excitotoxic at higher concentrations, to induce tissue damage. The protective effects of nicotine against glutamate induced neurotoxicity are demonstrated, comparing the IOS with NMDA and NMDA + nicotine. We additionally present data about the decrease of the propagation velocity of rSD waves after nicotine application and show analogical effects obtained with epibatidine, a specific nicotinic acetylcholine receptor (nAChR) agonist.
Biological cell membranes are complex structures containing mainly lipids and proteins. Functional aspects of such membranes are usually attributed to membrane integral proteins. However, it is well established that parameters of the lipid matrix are modifying the function of proteins. Additionally, electrical capacity and conductance of the plain lipid matrix of membranes are contributing directly to cellular functions as there is, for example, the propagation of action potentials. Accordingly the dependence of these parameters on changes of gravity might be important in the field of life sciences under space conditions. In this study consequently we have performed experiments in parabolic flight campaigns utilizing the patch-clamp technology to investigate conductance and capacity of plain lipid vesicle membranes under conditions of changing gravity. Both capacity and conductance were found to be gravity dependent. The changes in capacity could be contributed to changes in membrane geometry. Significant permeability in plain lipid membranes could be only observed at high potentials, where spontaneous current fluctuations occurred. The probability of these fluctuations was gravity dependent.
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