The Propagation of Mammalian Cells in a 20-Liter Stainless Steel Fermentor

Suspension culture has become the method of choice for the production of a wide range of therapeutic proteins and a number of vaccines. Some recombinant proteins, particularly antibodies, are required in quantities of hundreds of kilograms and even tons per annum. To meet these demands, industrial processes up to 20,000‐L scale have been developed based on stirred reactors, typically operated in batch or fed‐batch mode. For antibodies, optimization of fed‐batch processes has led to very high productivity, and yields of grams per liter are frequently reported. Continuous, perfused systems are also used. Several types of cells are used in these suspension processes, the most common being CHO. It is now possible to grow CHO and other cell types in large‐scale suspension culture using chemically defined media that contain no proteins or animal‐derived materials.

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Submerged culture of hamster kidney cells in a stainless steel vessel

Telling¹,

Elsworth²

1965

Biotech & Bioengineering

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SummaryThis paper describes the design and operation of an industrial type culture system for the submerged growth of BHK 21 cells a t a volume of 30 1. Using a modified Eagle's medium and with the culture controlled at pH 7.4 f 0.05 and at a temperature of 35 f 0.25"C., the cells grew from 0.5 X lo6 to about 2.5 X lo6 viable celLs/ml. in 50 hr. a t stirrer speeds of 330 and 460 rpm. The significance of certain aspects of design and operation of a culture system of this type is discussed.

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The Propagation of Mammalian Cells in a 20-Liter Stainless Steel Fermentor

Cited by 16 publications

References 14 publications

Suspension Culture, Animal Cells

Suspension Culture, Animal Cells

Suspension Culture, Animal Cells

Submerged culture of hamster kidney cells in a stainless steel vessel

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