Suspension Culture, Animal Cells

Abstract

Suspension culture has become the method of choice for the production of a wide range of therapeutic proteins and a number of vaccines. Some recombinant proteins, particularly antibodies, are required in quantities of hundreds of kilograms and even tons per annum. To meet these demands, industrial processes up to 20,000‐L scale have been developed based on stirred reactors, typically operated in batch or fed‐batch mode. For antibodies, optimization of fed‐batch processes has led to very high productivity, and yields of grams per liter are frequently reported. Continuous, perfused systems are also used. Several types of cells are used in these suspension processes, the most common being CHO. It is now possible to grow CHO and other cell types in large‐scale suspension culture using chemically defined media that contain no proteins or animal‐derived materials.

Animal Cell Lines as Expression Platforms in Viral Vaccine Production: A Post Covid-19 Perspective

“…The basis of all commercial applications based on cell culture focuses on the production of high amounts of cells to be used as an expression platform or for direct treatment in the field of personalized medicine. ,,, The reason for this is that there is a direct proportion between the amounts of end products such as biomass, monoclonal antibodies (mAb), growth factor, hormone, and viral vaccine that are desired to be obtained at the end of these productions and the total number of cells that can be reached during production. ,,,− …”

“… 9 , 29 , 74 , 75 The reason for this is that there is a direct proportion between the amounts of end products such as biomass, monoclonal antibodies (mAb), growth factor, hormone, and viral vaccine that are desired to be obtained at the end of these productions and the total number of cells that can be reached during production. 9 , 13 , 29 , 76 − 78 …”