The promotion of the vascularization of decalcified bone matrix in vivo by rabbit bone marrow mononuclear cell-derived endothelial cells

This study aimed to study the effects of initial cell density and in vitro culture method on the construction of tissue-engineered bone grafts and osteogenic activities. Human mesenchymal stem cells (hMSCs) were seeded onto cubic scaffolds prepared from demineralized bone matrix (DBM) by three methods - static, hydrodynamic, or fibrin hydrogel-assisted seeding. The resulting cell-scaffold constructs were cultured in vitro by static flask culture or hydrodynamic culture. The initial cell density and the subsequent in vitro proliferation and alkaline phosphate activities of the constructs were analyzed. The constructs were also subcutaneously implanted in nude mice to examine their in vivo osteogenic activities. Hydrogel-assisted seeding gave the highest seeding efficiency, followed by hydrodynamic and conventional static seeding. During in vitro culture, hydrodynamic culture produced higher plateau cell densities, alkaline phosphatase (ALP) activities, and extracellular matrix production than static culture. After subcutaneous implantation in nude mice, the implants prepared by the combination of hydrogel-assisted seeding and hydrodynamic culture produced higher wet weight and bone mineral density than implants prepared by other methods. The results suggest that the hydrogel-assisted seeding can substantially increase the initial seed cell density in scaffolds. Subsequent hydrodynamic culture can promote the proliferation and osteoblastic differentiation of the seeded cells. Correspondingly, bone grafts produced by the combination of these two methods achieved the highest osteogenic activity among the three methods employed.

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“…The porosity of DBM is 70% and the pore size is 300–800 µm. The DBM was prepared by a series process, as previously described by Tan et al [18].…”

Section: Methodsmentioning

confidence: 99%

Effects of Initial Cell Density and Hydrodynamic Culture on Osteogenic Activity of Tissue-Engineered Bone Grafts

et al. 2013

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“…Fibrin has been widely used to create vascularized tissue constructs for skin [62,63], adipose tissue [63][64][65][66], bone [67][68][69], cartilage [70], skeletal muscle [71], retina [72], liver [73], and cardiovascular [74,75] applications. The wide use of fibrin in vascularized tissues is due to its excellent pro-angiogenic properties.…”

Section: Fibrinmentioning

confidence: 99%

Biomaterials to Prevascularize Engineered Tissues

Tian

George

2011

J. of Cardiovasc. Trans. Res.

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Tissue engineering promises to restore tissue and organ function following injury or failure by creating functional and transplantable artificial tissues. The development of artificial tissues with dimensions that exceed the diffusion limit (1-2 mm) will require nutrients and oxygen to be delivered via perfusion (or convection) rather than diffusion alone. One strategy of perfusion is to prevascularize tissues; that is, a network of blood vessels is created within the tissue construct prior to implantation, which has the potential to significantly shorten the time of functional vascular perfusion from the host. The prevascularized network of vessels requires an extracellular matrix or scaffold for 3D support, which can be either natural or synthetic. This review surveys the commonly used biomaterials for prevascularizing 3D tissue engineering constructs.

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“…9 Second, vascularization is also a big challenge for large volume bone tissue engineering. 10,11 Third, popularly used autologous cell, with consideration of immunological advantage, makes tissue engineering impractical for widely clinical application, and less likely leads thetissue-engineered products to standardization and industrialization. Fourth, the trends of much more complicated artificial techniques towards to enhance osteogenesis and angiogenesis of implants, such as gene transfection, cytokine or drug release 3D scaffold design and vessels assembling, just make tissue engineering approach farther than the feasibility of widely-spread use in clinical practice.…”

Section: Introductionmentioning

confidence: 99%

Comparative study between tissue‐engineered periosteum and structural allograft in rabbit critical‐sized radial defect model

Zhao¹,

Zhao²,

Wang³

et al. 2011

J Biomed Mater Res

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The purpose of this study was to compare the efficacy of tissue-engineered periosteum (TEP) to allgeneic bone in repairing segmental bone defect. TEP was fabricated with osteoinduced rabbit bone marrow mesenchymal stem cells (MSCs) and porcine small intestinal submucosa (SIS). Allogrfats were cryopreserved radial segments of New Zealand Rabbits. Forty-eight radial critical-sized defects (CSD) were bilaterally produced in 24 rabbits. The defects were divided into three groups, group A, TEP implantation, group B, SIS implantation, and group C allograft. Bone defect reconstruction was kinetically analyzed at 4, 8, and 12 weeks by radiographic and histological scoring system. In group A, bone defects were radiographically and histologically healed with mature cortex and marrow cavity by 12 weeks, while none of the defects healed in group B. Group C showed a slow process of creeping substitution with lymphocyte infiltration. Statistical comparison confirmed that group A had a more efficient and rapid bone defect reparation as well as remodelling than Group B and C. In conclusion, TEP is superior to structural allograft in reconstruction of allogenic segmental bone defect. Pure SIS cannot guide bone regeneration in this rabbit model.

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The promotion of the vascularization of decalcified bone matrix in vivo by rabbit bone marrow mononuclear cell-derived endothelial cells

Cited by 19 publications

References 51 publications

Effects of Initial Cell Density and Hydrodynamic Culture on Osteogenic Activity of Tissue-Engineered Bone Grafts

Effects of Initial Cell Density and Hydrodynamic Culture on Osteogenic Activity of Tissue-Engineered Bone Grafts

Biomaterials to Prevascularize Engineered Tissues

Comparative study between tissue‐engineered periosteum and structural allograft in rabbit critical‐sized radial defect model

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