The promotion of osteoblastic differentiation of rat bone marrow stromal cells by a polyvalent plant mosaic virus

Kaur, Gagandeep; Valarmathi, Mani T.; Potts, Jay D.; Wang, Qin

doi:10.1016/j.biomaterials.2008.06.029

Cited by 76 publications

(82 citation statements)

References 66 publications

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“…A plant virus, turnip yellow mosaic virus (TYMV), was used as the model to demonstrate the reaction potential of these dyes in crosslinking two particles. Recent studies indicate that TYMV can be employed as a great tool in drug delivery, imaging and tissue engineering applications [6,[42][43][44][45][46]. This symmetric bionanoparticle (BNP) could self-assemble at the liquid-liquid interface to yield stable emulsion systems and highly ordered long range arrays at the flat interface [44].…”

Section: Introductionmentioning

confidence: 99%

Crosslinking of viral nanoparticles with “clickable” fluorescent crosslinkers at the interface

et al. 2010

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Cu (I) catalyzed alkyne-azide cycloaddition (CuAAC) reaction, a typical "click" reaction, is one of the modular synthetic approaches which has been broadly used in various organic syntheses, medicinal chemistry, materials development and bioconjugation applications. We have for the first time synthesized two dialkyne derivatized fluorescent crosslinkers which could be applied to crosslink two biomolecules using CuAAC reaction. Turnip yellow mosaic virus, a plant virus with unique structural and chemical properties, was used as a prototypical scaffold to form a 2D single layer at the interface of two immiscible liquids and crosslinked with these two linkers by the CuAAC reaction. Upon crosslinking, the fluorescence of both linkers diminished, likely due to the distortion of the polymethylene backbone, which therefore could be used to indicate the completion of the reaction.

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Section: Introductionmentioning

confidence: 99%

Crosslinking of viral nanoparticles with “clickable” fluorescent crosslinkers at the interface

et al. 2010

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“…23 Other studies have shown that the presence of nanorods or nanoparticles on the substrates markedly upregulated the expression of a set of genes involved in the differentiation of osteoblast and the subsequent functions of cells, including Smo, Ihh, and Gli1. 24,25 These data indicate a potential role of the Hedgehog signaling pathway in the enhanced osteoblast adhesion, growth, and differentiation via MNTs.…”

Section: Introductionmentioning

confidence: 82%

“…Recent studies have shown that the activity of the Hedgehog signaling was sensitive to the surface properties of biomedical implants; the surface micro-/nanotexture on biomaterials induced a significant upregulation of Hedgehog-Gli1 signaling. [18][19][20][23][24][25] Given the evidence implicating significant roles of the Hedgehog signaling in osteoblast proliferation and differentiation and bone formation, we investigated the role of Hedgehog signaling in the MG63 osteoblast proliferation and differentiation caused by MNTs. Our results indicated that the enhanced proliferation and differentiation of MG63 osteoblasts by MNTs are associated with the increased gene expression and protein production of Shh, Smo, and Gli1 as well as the activation of Hedgehog signaling, suggesting that the biological effects of MNTs may be mediated by the Hedgehog signaling pathway.…”

Section: Discussionmentioning

confidence: 99%

“…18,19 Other studies have indicated that MNTs induce an obvious activation of several functional osteogenesis-related signaling pathways, including Hedgehog signaling, resulting in an enhanced osteoblast differentiation, maturation, and bone formation. [23][24][25]38 Consequently, we conducted this study to systematically estimate the Hedgehog-Gli1 signaling response to different titanium topographies. Our data showed that the MNTs induced higher gene and protein expression of Shh, Smo, and Gli1 and the activation of Hedgehog signaling compared to smooth and microstructured surfaces.…”

Section: Discussionmentioning

confidence: 99%

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Role of Hedgehog–Gli1 signaling in the enhanced proliferation and differentiation of MG63 cells enabled by hierarchical micro-/nanotextured topography

Lin¹,

Huang²,

He³

et al. 2017

IJN

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Hedgehog–Gli1 signaling is evolutionarily conserved and plays an essential role in osteoblast proliferation and differentiation as well as bone formation. To evaluate the role of the Hedgehog–Gli1 pathway in the response of osteoblasts to hierarchical biomaterial topographies, human MG63 osteoblasts were seeded onto smooth, microstructured, and micro-/nanotextured topography (MNT) titanium to assess osteoblast proliferation and differentiation in terms of proliferative activity, alkaline phosphatase (ALP) production, and osteogenesis-related gene expression. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the mRNA expression of Sonic hedgehog (Shh), Smoothened (Smo), and Gli1, and the protein levels were assayed by Western blotting. MG63 cells treated with the Smo inhibitor cyclopamine were seeded onto the titanium specimens, and the cell proliferation and differentiation were studied in the presence or absence of cyclopamine. Our results showed that compared to the smooth and microstructured surfaces, the MNTs induced a higher gene expression and protein production of Shh, Smo, and Gli1 as well as the activation of Hedgehog signaling. The enhanced proliferative activity, ALP production, and expression of the osteogenesis-related genes (bone morphogenetic protein-2, ALP, and runt-related transcription factor 2) enabled by the MNTs were significantly downregulated by the presence of cyclopamine to a similar level as those on the smooth and acid-etched microstructured surfaces in the absence of cyclopamine. This evidence explicitly demonstrates pivotal roles of Hedgehog–Gli1 signaling pathway in mediating the enhanced effect of MNTs on MG63 proliferation and differentiation, which greatly advances our understanding of the mechanism involved in the biological responsiveness of biomaterial topographies. These findings may aid in the optimization of hierarchical biomaterial topographies targeting Hedgehog–Gli1 signaling.

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“…Of all the osteo-related genes, OCN is regarded as the most specific marker for the mature osteoblast and mineralization during the course of osteogenesis. 30 It accumulates in the calcified bone due to its high affinity to hydroxyapatite crystals. As shown in Figure 4C, OCN gene was upregulated in A-pDA group in comparison with R-pDA group (P,0.05).…”

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confidence: 99%

Osteoinductive peptide-functionalized nanofibers with highly ordered structure as biomimetic scaffolds for bone tissue engineering

Gao¹,

Zhang²,

Song³

et al. 2015

IJN

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The construction of functional biomimetic scaffolds that recapitulate the topographical and biochemical features of bone tissue extracellular matrix is now of topical interest in bone tissue engineering. In this study, a novel surface-functionalized electrospun polycaprolactone (PCL) nanofiber scaffold with highly ordered structure was developed to simulate the critical features of native bone tissue via a single step of catechol chemistry. Specially, under slightly alkaline aqueous solution, polydopamine (pDA) was coated on the surface of aligned PCL nanofibers after electrospinning, followed by covalent immobilization of bone morphogenetic protein-7-derived peptides onto the pDA-coated nanofiber surface. Contact angle measurement, Raman spectroscopy, and X-ray photoelectron spectroscopy confirmed the presence of pDA and peptides on PCL nanofiber surface. Our results demonstrated that surface modification with osteoinductive peptides could improve cytocompatibility of nanofibers in terms of cell adhesion, spreading, and proliferation. Most importantly, Alizarin Red S staining, quantitative real-time polymerase chain reaction, immunostaining, and Western blot revealed that human mesenchymal stem cells cultured on aligned nanofibers with osteoinductive peptides exhibited enhanced osteogenic differentiation potential than cells on randomly oriented nanofibers. Furthermore, the aligned nanofibers with osteoinductive peptides could direct osteogenic differentiation of human mesenchymal stem cells even in the absence of osteoinducting factors, suggesting superior osteogenic efficacy of biomimetic design that combines the advantages of osteoinductive peptide signal and highly ordered nanofibers on cell fate decision. The presented peptide-decorated bone-mimic nanofiber scaffolds hold a promising potential in the context of bone tissue engineering.

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The promotion of osteoblastic differentiation of rat bone marrow stromal cells by a polyvalent plant mosaic virus

Cited by 76 publications

References 66 publications

Crosslinking of viral nanoparticles with “clickable” fluorescent crosslinkers at the interface

Crosslinking of viral nanoparticles with “clickable” fluorescent crosslinkers at the interface

Role of Hedgehog–Gli1 signaling in the enhanced proliferation and differentiation of MG63 cells enabled by hierarchical micro-/nanotextured topography

Osteoinductive peptide-functionalized nanofibers with highly ordered structure as biomimetic scaffolds for bone tissue engineering

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The promotion of osteoblastic differentiation of rat bone marrow stromal cells by a polyvalent plant mosaic virus

Cited by 76 publications

References 66 publications

Crosslinking of viral nanoparticles with “clickable” fluorescent crosslinkers at the interface

Crosslinking of viral nanoparticles with “clickable” fluorescent crosslinkers at the interface

Role of Hedgehog&ndash;Gli1 signaling in the enhanced proliferation and differentiation of MG63 cells enabled by hierarchical micro-/nanotextured topography

Osteoinductive peptide-functionalized nanofibers with highly ordered structure as biomimetic scaffolds for bone tissue engineering

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Role of Hedgehog–Gli1 signaling in the enhanced proliferation and differentiation of MG63 cells enabled by hierarchical micro-/nanotextured topography