The promoters of 3 celery salt-induced phloem-specific genes as new tools for monitoring salt stress responses

Landouar-Arsivaud, Lucie; Juchaux-Cachau, Marjorie; Jeauffre, Julien; Biolley, Jean-Philippe; Maurousset, Laurence; Lemoine, Rémi

doi:10.1016/j.plaphy.2010.09.008

Cited by 13 publications

(6 citation statements)

References 38 publications

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“…Glucose, fructose and sucrose were extracted from approximately 10 mg of tissue sample previously lyophilized, by three washings (1.5 mL and twice 0.5 mL) in methanol:chloroform:water (MCW) (12:5:3, by vol.). Supernatants containing soluble sugars were pooled and mixed with 0.6 volume of water and centrifuged (Landouar-Arsivaud et al 2010 ). The upper aqueous phase was collected and evaporated at 30 °C with a MiVac Quattro (Genevac, Ipswich, UK).…”

Section: Methodsmentioning

confidence: 99%

Carbon source–sink relationship in Arabidopsis thaliana: the role of sucrose transporters

Durand¹,

Mainson²,

Porcheron³

et al. 2017

Planta

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165

113

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Main conclusion The regulation of source-to-sink sucrose transport is associated with AtSUC and AtSWEET sucrose transporters’ gene expression changes in plants grown hydroponically under different physiological conditions. Source-to-sink transport of sucrose is one of the major determinants of plant growth. Whole-plant carbohydrates’ partitioning requires the specific activity of membrane sugar transporters. In Arabidopsis thaliana plants, two families of transporters are involved in sucrose transport: AtSUCs and AtSWEETs. This study is focused on the comparison of sucrose transporter gene expression, soluble sugar and starch levels and long distance sucrose transport, in leaves and sink organs (mainly roots) in different physiological conditions (along the plant life cycle, during a diel cycle, and during an osmotic stress) in plants grown hydroponically. In leaves, the AtSUC2, AtSWEET11, and 12 genes known to be involved in phloem loading were highly expressed when sucrose export was high and reduced during osmotic stress. In roots, AtSUC1 was highly expressed and its expression profile in the different conditions tested suggests that it may play a role in sucrose unloading in roots and in root growth. The SWEET transporter genes AtSWEET12, 13, and 15 were found expressed in all organs at all stages studied, while differential expression was noticed for AtSWEET14 in roots, stems, and siliques and AtSWEET9, 10 expressions were only detected in stems and siliques. A role for these transporters in carbohydrate partitioning in different source–sink status is proposed, with a specific attention on carbon demand in roots. During development, despite trophic competition with others sinks, roots remained a significant sink, but during osmotic stress, the amount of translocated [U-14C]-sucrose decreased for rosettes and roots. Altogether, these results suggest that source–sink relationship may be linked with the regulation of sucrose transporter gene expression.Electronic supplementary materialThe online version of this article (10.1007/s00425-017-2807-4) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

Carbon source–sink relationship in Arabidopsis thaliana: the role of sucrose transporters

Durand¹,

Mainson²,

Porcheron³

et al. 2017

Planta

Self Cite

165

113

View full text Add to dashboard Cite

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“…This is probably the reason why salinity resistance/tolerance is commonly found in polyol synthesizing plants. Furthermore, the increased polyol synthesis together with an increased expression of genes encoding polyol transporters was observed in the phloem of Plantago major (Pommerrenig et al, 2007), Apium graveolens (Landouar-Arsivaud et al, 2011), and Olea europaea (Conde et al, 2011), suggesting that long-distance polyol transport is induced/enhanced by salinity stress. The translocation of polyols from shoots to roots may positively affect root metabolism and water potential.…”

Section: Source/sink Dynamicsmentioning

confidence: 95%

Drought and Salinity Stress Responses and Microbe-Induced Tolerance in Plants

2020

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“…Polyols are considered as major molecules for plants to cope with stress (Stoop et al, 1996). In polyol-transporting plants, increased polyol synthesis occurs together with an increased expression of genes encoding polyol transporters located in the phloem in Plantago (Pommerrenig et al, 2007), celery (Landouar-Arsivaud et al, 2011) and olive (Conde et al, 2011), suggesting that long-distance polyol transport is also enhanced in response to salt stress. Increased delivery of polyols to roots could have a positive effect on metabolism and water potential of roots.…”

Section: Effects Of Abiotic Factorsmentioning

confidence: 99%

Source-to-sink transport of sugar and regulation by environmental factors

Lemoine¹,

Camera²,

Atanassova³

et al. 2013

Front. Plant Sci.

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Source-to-sink transport of sugar is one of the major determinants of plant growth and relies on the efficient and controlled distribution of sucrose (and some other sugars such as raffinose and polyols) across plant organs through the phloem. However, sugar transport through the phloem can be affected by many environmental factors that alter source/sink relationships. In this paper, we summarize current knowledge about the phloem transport mechanisms and review the effects of several abiotic (water and salt stress, mineral deficiency, CO2, light, temperature, air, and soil pollutants) and biotic (mutualistic and pathogenic microbes, viruses, aphids, and parasitic plants) factors. Concerning abiotic constraints, alteration of the distribution of sugar among sinks is often reported, with some sinks as roots favored in case of mineral deficiency. Many of these constraints impair the transport function of the phloem but the exact mechanisms are far from being completely known. Phloem integrity can be disrupted (e.g., by callose deposition) and under certain conditions, phloem transport is affected, earlier than photosynthesis. Photosynthesis inhibition could result from the increase in sugar concentration due to phloem transport decrease. Biotic interactions (aphids, fungi, viruses…) also affect crop plant productivity. Recent breakthroughs have identified some of the sugar transporters involved in these interactions on the host and pathogen sides. The different data are discussed in relation to the phloem transport pathways. When possible, the link with current knowledge on the pathways at the molecular level will be highlighted.

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The promoters of 3 celery salt-induced phloem-specific genes as new tools for monitoring salt stress responses

Cited by 13 publications

References 38 publications

Carbon source–sink relationship in Arabidopsis thaliana: the role of sucrose transporters

Carbon source–sink relationship in Arabidopsis thaliana: the role of sucrose transporters

Drought and Salinity Stress Responses and Microbe-Induced Tolerance in Plants

Source-to-sink transport of sugar and regulation by environmental factors

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