The Promoter Region Polymorphism of the Tasap-A1 Gene in the Collection of Winter Wheat (Triticum Aestivum L.) Varieties and Lines and Its Influence on Agronomic Traits

Abstract

During the study of the promoter region of the TaSAP-Al gene on the allelic composition of Sap5, Sap39 and Sap2606 loci, it was shown that winter wheat varieties and lines from the collection used in the selection process in the Republic of Belarus in 2014 refer to 4 haplotypes and most of the tested samples (45.6 %) belongs to the haplotype II. It was found that the haplotype IV contributes to an increase in thousand-kernel weight and yield by 5.2 and 9.2 %, and haplotype I – an increase in spike length and total number of spikelets per spike by 4.4 and 2.2 % compared with the average values of these indicators. It was also found that the presence of deletion at position –1810 bp has a positive effect on spike length, total number of spikelets per spike and yield, the presence of insertion at position –1637 bp – spike length, and the presence of deletion in this position – on yield. The positive effect of SNP-2606C at position –2606 bp on thousand-kernel weight, spike length and yield was shown.

Expression of Specific Alleles of Zinc-Finger Transcription Factors, HvSAP8 and HvSAP16, and Corresponding SNP Markers, Are Associated with Drought Tolerance in Barley Populations

“…The results (Figure 5a,c,e) showed a strong association of the Granal haplotype with higher GY and TGW, similar to results for TaSAP1-A1 in bread wheat, OsSAP8 in rice and HvSAP8 in barley. A strong association between haplotype III (based on SNPs) in the promoter region and high TGW as a component of GY in dry field trials, was shown earlier [24][25][26][27].…”

“…Based on single nucleotide polymorphism (SNP) in the promoter region of TaSAP1-A1, molecular markers were developed and successfully used to select targeted genotypes. Significantly higher TGW was associated with specific haplotypes of TaSAP1-A1 in China [25], Europe [26], and Belarus [27]. Importantly, based on the provided sequence accession number (KC193579) for TaSAP1-A1 in the NCBI database [24], the protein accession AGU01535 was retrieved and identified as being the closest homolog to polypeptides of OsSAP8 in rice and HvSAP8 in barley.…”