Expression of Specific Alleles of Zinc-Finger Transcription Factors, HvSAP8 and HvSAP16, and Corresponding SNP Markers, Are Associated with Drought Tolerance in Barley Populations

Abstract

Two genes, HvSAP8 and HvSAP16, encoding Zinc-finger proteins, were identified earlier as active in barley plants. Based on bioinformatics and sequencing analysis, six SNPs were found in the promoter regions of HvSAP8 and one in HvSAP16, among parents of two barley segregating populations, Granal × Baisheshek and Natali × Auksiniai-2. ASQ and Amplifluor markers were developed for HvSAP8 and HvSAP16, one SNP in each gene, and in each of two populations, showing simple Mendelian segregation. Plants of F6 selected breeding lines and parents were evaluated in a soil-based drought screen, revealing differential expression of HvSAP8 and HvSAP16 corresponding with the stress. After almost doubling expression during the early stages of stress, HvSAP8 returned to pre-stress level or was strongly down-regulated in plants with Granal or Baisheshek genotypes, respectively. For HvSAP16 under drought conditions, a high expression level was followed by either a return to original levels or strong down-regulation in plants with Natali or Auksiniai-2 genotypes, respectively. Grain yield in the same breeding lines and parents grown under moderate drought was strongly associated with their HvSAP8 and HvSAP16 genotypes. Additionally, Granal and Natali genotypes with specific alleles at HvSAP8 and HvSAP16 were associated with improved performance under drought via higher 1000 grain weight and more shoots per plant, respectively.

Designing Allele-Specific Competitive-Extension PCR-Based Assays for High-Throughput Genotyping and Gene Characterization

“…These results for SNP for genotyping of humans validated our conclusion that the proposed ASQ method is very accurate and effective for SNP genotyping; we expect similar results in other animal species. In addition, we developed ASQ sets and applied SNP genotyping in the barley genes HvSAP16 and HvSAP8 (controlling stress-associated proteins); these are validated examples and have been accepted for publication ( Baidyussen et al, 2021 ; Kalendar et al, 2022 ). Our tool and the ASQ method are suitable for two-, three-, or four-allelic uniplex reactions but can potentially be used for different SNPs in a multiplex format in a range of applications, including medical or forensic studies or other studies involving SNP genotyping.…”

Identification and promoter analysis of a GA-stimulated transcript 1 gene from Jatropha curcas

“…IAA9 has been reported to act as a transcriptional repressor of auxin signaling in tomato (Wang et al 2005). AHL19 and SAP8 have been described to be involved in stress resistance in Arabidopsis and barley, respectively (Yadeta et al 2011;Baidyussen et al 2021). Due to the promotional effect of JcGAST1 on plant growth, there may be some regulatory factors that repress GAST1 to maintain energy to ensure that the organism can survive in adverse environments.…”

Spring Barley Hybrids Assessment for Biological and Economic Features Under Drought Conditions of Northern and Central Kazakhstan

“…New cultivars' development considers the relationship of the crucial economically valuable parameters and comprehensively assesses them at all stages of the breeding process. Employing various breeding methods to develop the base material includes advances in molecular biology; however, conventional hybridization is still one of the chief breeding methods (Lisitsyn, 2018;Baidyussen et al, 2021). In the forest-steppe zone of Western Siberia, using intraspecific hybridization, creating the spring barley cultivar 'Tolkan' was for drought and lodging resistance, immunity to smut fungi, and dusty and hard smut, averaging a yield of 5.4 t/ha, which exceeded the standard cultivar by 0.8 t/ha (Pakul and Martynova, 2020).…”