Members of Gibberellic acid-stimulated Arabidopsis (GASA) gene family play roles in plant growth and development, particularly in flower induction and seed development. However, there is still relatively limited knowledge of GASA genes in Jatropha curcas. Herein, we identified a GASA family gene from Jatropha curcas, namely JcGAST1, which encodes a protein containing a conserved GASA domain. Sequence alignment showed that JcGASAT1 protein shares 76% sequence identity and 80% sequence similarity with SlGAST1. JcGAST1had higher expression and protein levels in the male flowers than in the female flowers. Overexpression of JcGAST1 in tobacco promotes plant growth but inhibits pistil development. JcGAST1 expression was upregulated by GA and downregulated by MeJA. Promoter analysis indicated that the pyrimidine box and CGTCA motif were the GA-and MeJA-responsive elements of the JcGAST1 promoter. Using a Y1H screen, six transcription factors were found to interact with the pyrimidine box, and three transcription factors were found to interact with theCGTCA motif. Overall, the results of this study improve our understanding of the JcGAST1 gene and provide useful information for further studies.
Background The gibberellic acid-stimulated Arabidopsis (GASA) gene encodes a class of cysteine-rich functional proteins and is ubiquitous in plants. Most GASA proteins are influence the signal transmission of plant hormones and regulate plant growth and development, however, their function in Jatropha curcas is still unknown. Results In this study, we cloned JcGASA6, a member of the GASA family, from J. curcas. The JcGASA6 protein has a GASA-conserved domain and is located in the tonoplast. The three-dimensional structure of the JcGASA6 protein is highly consistent with the antibacterial protein Snakin-1. Additionally, the results of the yeast one-hybrid (Y1H) assay showed that JcGASA6 was activated by JcERF1, JcPYL9, and JcFLX. The results of the Y2H assay showed that both JcCNR8 and JcSIZ1 could interact with JcGASA6 in the nucleus. The expression of JcGASA6 increased continuously during male flower development, and the overexpression of JcGASA6 was associated with filament elongation of the stamens in tobacco. Conclusion JcGASA6, a member of the GASA family in J. curcas, play an important role in growth regulation and floral development (especially in male flower). It is also involved in the signal transduction of hormones, such as ABA, ET, GA, BR, and SA. Also, JcGASA6 is a potential antimicrobial protein determined by its three-dimensional structure.
GASA encodes a class of cysteine-rich functional proteins and widely exists in plants. Most GASA proteins are involved in the signal transmission of plant hormones and regulate plant growth and development, but their function in Jatropha curcas is still unknown. In our study, we cloned a member of GASA family from J. curcas-JcGASA6. JcGASA6 protein has a GASA conserved domain and is located in tonoplast. The three-dimensional structure of JcGASA6 protein is highly consistent with antibacterial protein Snakin-1. Moreover, JcGASA6 was activated by JcERF1, JcPYL9 and JcFLX. Both JcCNR8 and JcSIZ1 can interact with JcGASA6 in the nucleus. The expression of JcGASA6 increased continuously during male flower development, and overexpression of JcGASA6 cause filament elongation of stamen in tobacco. All our results reveal the function of JcGASA6 from the protein and gene levels, and enriched signal network of GASA Protein in hormone crosstalk.
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