The promoter of the oocyte-specific gene, Gdf9, is active in population of cultured mouse embryonic stem cells with an oocyte-like phenotype

Salvador, Lisa; Silva, Celso P.; Kostetskii, Igor; Radice, Glenn L.; Strauss, Jerome F.

doi:10.1016/j.ymeth.2008.03.004

Cited by 43 publications

(32 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In ESCs stably transfected with the promoter region of the mouse Gdf9 coupled to enhanced green fluorescent protein (eGFP) and cultured on feeder cell layers, large eGFP-positive cells, surrounded by a structure resembling a zona pellucida appeared transiently and very rapidly on the first days of culture. However, in EB cultures, eGFP-positive cells appeared transiently and then reappeared in regional clusters after a relatively long culture of 30-45 days (Salvador et al, 2008). Finally, putative PGCs obtained from 21 day EBs reaggregated with newborn ovarian tissues and transplanted under the kidney capsule of recipient mice give rise to oocytes enclosed in primary follicle at very low frequency (0.023%) (Nicholas et al, 2009).…”

Section: Discussionmentioning

confidence: 98%

Impaired meiotic competence in putative primordial germ cells produced from mouse embryonic stem cells

Tedesco

Farini²,

Felici³

2011

Int. J. Dev. Biol.

View full text Add to dashboard Cite

There are still several unanswered questions and problems about the recently claimed possibility of producing functional germ cells in vitro from pluripotent embryonic stem cells (ESCs). In the present paper, we compared by single-cell analysis the capability of putative primordial germ cells (PGCs), produced in vitro from ESCs, and that of endogenous PGCs isolated from embryos, to enter and progress through meiotic prophase I. Using a protocol previously reported to be suitable to produce female germ cells from mouse ESC monolayers, we first identified putative PGCs by analysing the expression pattern of several markers such as SSEA1, APase, OCT4, NANOG, MVH and SCP3 of pre-and post migratory PGCs. Next, after isolation of such cells from culture, we tested their meiotic capability. The evaluation at 2-5 days of culture of the number of cells showing meiotic nuclear SCP3 staining in cytospreads showed that it remained nearly constant in the putative PGCs, whereas it increased markedly in endogenous PGCs. Moreover, we observed that in putative PGCs, the nuclear distribution or expression of SCP3 and other meiotic markers such as DMC1,     H2AX and SCP1 were always highly abnormal in comparison to that observed in endogenous cultured PGCs. We conclude that although the formation of cells showing characteristics of PGCs can occur efficiently from ESCs in vitro, these cells possess impaired capability to enter and progress through meiotic prophase I. KEY WORDS: embryonic stem cell, gametogenesis, meiosis, primordial germ cellFrom the first report by Hübner et al. (2003) showing that XY mouse embryonic stem cells (mESCs) are able to produce follicleenclosed growing oocytes, several studies have described the in vitro differentiation of female and male germ cells from mouse and human ESCs (hESC) in either the monolayer or the embryoid bodies (EBs) culture method (for reviews see Hua and Sidhu, 2008;Nagano, 2007;Aflatoonian and Moore, 2006). The experiments carried out to produce germ cells from ESCs are based on years of intense investigations on the in vivo and in vitro development of mouse primordial germ cells (PGCs), the oocyte and sperm precursors (for reviews see De Felici et al., 2004;De Felici, 2001). In particular the recent progress in elucidating the process of germ line specification in mammals (Ohinata et al., 2009 and references herein) and the possibility to mimic in vitro events of later PGC development including entering into meiosis (Farini et al., 2005), have given precious information for the derivation and characterization of germ cells produced from ESCs.In the mouse embryo, PGC development occurs in about 7Int. J. Dev. Biol. 55: 215-222 (2011) days, from around 5.5 days post coitus (dpc), when the first inductive events of the germ cell lineage occur in the epiblast, to 12.5 dpc when sex differentiation of PGCs into meiotic oocytes in the female and G0-arrested prospermatogoni/gonocytes in maleoccur (for a review, see Western, 2009). Elegant recent studies have shown that m...

show abstract

Section: Discussionmentioning

confidence: 98%

Impaired meiotic competence in putative primordial germ cells produced from mouse embryonic stem cells

Tedesco

Farini²,

Felici³

2011

Int. J. Dev. Biol.

View full text Add to dashboard Cite

show abstract

“…These structures expressed a set of stage-specific molecular trophectodermal markers, such as Hand1, Pl-1, Mash-2 and TpBp. Morulae-like structures showed signal of Oct-4 protein with appropriate nuclear localization, which differs from GC where Oct-4 localizes in the cytoplasm (Hübner et al, 2003;Salvador et al, 2008). Native mES cells (XY) without any genetic modification also were able to produce putative oocytes.…”

Section: Derivation Of Female Gc and Artificial Gametesmentioning

confidence: 98%

Actual Achievements on Germ Cells and Gametes Derived from Pluripotent Stem Cells

Kerkis¹,

Mendes²,

Fonseca³

et al. 2011

Embryonic Stem Cells - Recent Advances in Pluripotent Stem Cell-Based Regenerative Medicine

View full text Add to dashboard Cite

“…However, these animals were only able to survive for five months, most likely due to impaired sex-specific imprinting in mESCderived male gametes (Nayernia et al 2006). Several other groups have tried different differentiation strategies and growth factor cocktails, but the imprinting status, germ cell maturation and meiosis in vitro remain difficult obstacles to produce functional mESC-derived gametes (Lacham-Kaplan et al 2006;Kerkis et al 2007;Qing et al 2007;Salvador et al 2008). The recent breakthrough work of Hayashi and colleagues has provided a promising future to the germ cell differentiation potential of ESCs (Hayashi et al 2011(Hayashi et al , 2012.…”

Section: Embryonic Stem Cells Derived Germ Cells and Gametesmentioning

confidence: 99%

Use of pluripotent stem cells for reproductive medicine: are we there yet?

Duggal

Heindryckx

Deroo

et al. 2014

Veterinary Quarterly

View full text Add to dashboard Cite

In recent years, pluripotent stem cells have demonstrated to be exciting tools to understand embryonic development, cell lineage specification, tissue generation and repair, and various other biological processes. In addition, the identification and isolation of germ line stem cells has given more insight into germ cell biology at the molecular level and into the underlying causes of infertility which was not possible earlier. The recent derivation of in vitro derived sperm and oocytes from pluripotent stem cells in the mouse model represents a major breakthrough in the field and substantiates the critical relevance of stem cells as a potential alternative resource for treating infertility. Although the past years have yielded compelling information in understanding germ cell development via in vitro stem cell assays, extended investigative research is necessary in order to derive fully functional 'artificial gametes' in a safe way for future therapeutic applications.

show abstract

The promoter of the oocyte-specific gene, Gdf9, is active in population of cultured mouse embryonic stem cells with an oocyte-like phenotype

Cited by 43 publications

References 44 publications

Impaired meiotic competence in putative primordial germ cells produced from mouse embryonic stem cells

Impaired meiotic competence in putative primordial germ cells produced from mouse embryonic stem cells

Actual Achievements on Germ Cells and Gametes Derived from Pluripotent Stem Cells

Use of pluripotent stem cells for reproductive medicine: are we there yet?

Contact Info

Product

Resources

About