The prognostic value of proliferating cell nuclear antigen (PCNA) in the advanced cancer of larynx

Dobroś, W; Ryś, Janusz; Niezabitowski, A; Olszewski, E

doi:10.1016/s0385-8146(98)00032-7

Cited by 12 publications

(8 citation statements)

References 12 publications

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“…We observed an inverse correlation between PCNA index and tumour grading. These findings are consistent with some studies [14, 16, 20, 21] and in contradiction to others [12, 22]. More detailed histological examinations showed that PCNA immunostaining corresponds to the histological grade of benign epithelial hyperplastic lesions and squamous cell carcinoma of the larynx [18, 19].…”

Section: Discussionsupporting

confidence: 90%

Preliminary results of prognostic significance of proliferating cell nuclear antigen expression in advanced primary larynx carcinomas and lymph node metastases

Kupisz¹,

Stepulak²,

Zdunek³

et al. 2010

aoms

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IntroductionThe aim of this study was to investigate the prognostic significance of proliferating cell nuclear antigen (PCNA) expression in laryngeal carcinoma in relation to clinicopathological features. Special emphasis was placed on examining the relationship of PCNA expression in the primary tumour and PCNA expression in corresponding lymph node metastases obtained from the same patients.Material and methodsThe study included 60 patients with advanced larynx carcinoma who had received treatment and follow-up for at least 5 years. Sixty laryngeal carcinoma specimens and metastatic lymph nodes from 24 patients were examined for immunohistochemical PCNA expression.ResultsThe percentages of PCNA positive cells were significantly higher in the primary tumours which developed lymph node metastases than in those without metastases. The fraction of PCNA immunolabelled cells in metastatic lymph nodes increased significantly when compared with the PCNA positive cell score in their corresponding primary tumours obtained from the same patient. There was a significant difference in PCNA index score in primary tumours between the group of patients who survived a 5-year period and those who died within 5 years after treatment.ConclusionsOur data demonstrate that a high proliferation index in primary larynx tumours is retained and increased in corresponding lymph node metastases. Measurement of the fraction of cancer cells stained for PCNA in primary larynx carcinomas can be helpful in selecting tumours with high aggressiveness potential that are more likely to develop neck metastases and thereby in identifying patients who need elective lymph node dissection or additional treatment.

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Section: Discussionsupporting

confidence: 90%

Preliminary results of prognostic significance of proliferating cell nuclear antigen expression in advanced primary larynx carcinomas and lymph node metastases

Kupisz¹,

Stepulak²,

Zdunek³

et al. 2010

aoms

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“…neck metastasis and prognosis (17). but not with differentiation (7). As cell proliferation is one ofthe central charaetcristies of tumor development and prt>gression.…”

Section: Discussionmentioning

confidence: 99%

“…Analysis of cell prohferation has provided additional information on tumor behavior in many tissues, including the larynx (5)(6)(7). Immunohistochemical expression of certain proteins is associated with cell proliferation related to laryngeal neoplasm development and behavior (8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20).…”

Section: Introductionmentioning

confidence: 99%

Automated image analysis of proliferating cells in carcinoma of the larynx

Laitakari

Harrison

Stenbäck

2003

Acta Oto-Laryngologica

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“…It is also central for assessment of the malignancy of tumours. Usually, cell proliferation is estimated by immunolabeling of endogenous nuclear proteins, which are up regulated during the progression through the cell cycle, such as cyclin D1, the proliferating cell nuclear antigen (PCNA) or Ki67 (Bartkova et al 1995; Dobros et al 1998; Trihia et al 2003). The detection of thymidine analogues incorporated into newly synthesized DNA is commonly used to reveal DNA synthesis (Miller and Nowakowski 1988).…”

Section: Introductionmentioning

confidence: 99%

Immunolocalization of phospho-S6 kinases: a new way to detect mitosis in tissue sections and in cell culture

Schmidt

Wahl

Wüthrich

et al. 2006

Histochem Cell Biol

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During a study on the mTor pathway in the rat kidney we observed a striking increase of the phosphorylation of the S6 kinase in mitosis. In cryostat sections of perfusion-Wxed tissue mitotic cells appeared as bright spots in immunoXuorescence using an antibody speciWc for the phosphorylation site Thr421/Ser424. They were easily spotted in overviews with the objective 4£ and 10£. ImmunoXuorescence was weak during the interphase. During the prophase it increased in both the nucleus and the cytoplasm and it remained bright during the subsequent phases of mitosis. All mitotic cells which were found in tubules and in the interstitium of the kidney using a chromatin stain displayed the bright immunoXuorescence for phospho-S6 kinase. The same phenomenon was observed in rat liver and in mouse kidney as well as in a human cell line. Provided a rapid Wxation, mitotic cells could be identiWed with the immunoperoxidase technique in paraYn sections of immersion-Wxed tissue. This is the Wrst report of phosphorylation of S6 kinase during mitosis in vivo. Thus, immunohistochemistry with antiphospho-S6 kinase (Thr421/Ser424) appears to provide a convenient way to detect mitotic cells at low magniWcation.

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The prognostic value of proliferating cell nuclear antigen (PCNA) in the advanced cancer of larynx

Cited by 12 publications

References 12 publications

Preliminary results of prognostic significance of proliferating cell nuclear antigen expression in advanced primary larynx carcinomas and lymph node metastases

Preliminary results of prognostic significance of proliferating cell nuclear antigen expression in advanced primary larynx carcinomas and lymph node metastases

Automated image analysis of proliferating cells in carcinoma of the larynx

Immunolocalization of phospho-S6 kinases: a new way to detect mitosis in tissue sections and in cell culture

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