Preliminary results of prognostic significance of proliferating cell nuclear antigen expression in advanced primary larynx carcinomas and lymph node metastases

Abstract

IntroductionThe aim of this study was to investigate the prognostic significance of proliferating cell nuclear antigen (PCNA) expression in laryngeal carcinoma in relation to clinicopathological features. Special emphasis was placed on examining the relationship of PCNA expression in the primary tumour and PCNA expression in corresponding lymph node metastases obtained from the same patients.Material and methodsThe study included 60 patients with advanced larynx carcinoma who had received treatment and follow-up for at least 5 years. Sixty laryngeal carcinoma specimens and metastatic lymph nodes from 24 patients were examined for immunohistochemical PCNA expression.ResultsThe percentages of PCNA positive cells were significantly higher in the primary tumours which developed lymph node metastases than in those without metastases. The fraction of PCNA immunolabelled cells in metastatic lymph nodes increased significantly when compared with the PCNA positive cell score in their corresponding primary tumours obtained from the same patient. There was a significant difference in PCNA index score in primary tumours between the group of patients who survived a 5-year period and those who died within 5 years after treatment.ConclusionsOur data demonstrate that a high proliferation index in primary larynx tumours is retained and increased in corresponding lymph node metastases. Measurement of the fraction of cancer cells stained for PCNA in primary larynx carcinomas can be helpful in selecting tumours with high aggressiveness potential that are more likely to develop neck metastases and thereby in identifying patients who need elective lymph node dissection or additional treatment.

Increased level of serum prostaglandin-2 in early stage of esophageal squamous cell carcinoma

“…However, ROC analysis showed that the use of serum PGE-2 concentration as a biomarker in ESCC is doubtful. The diagnostic parameters for PGE-2 were significantly lower than for other tested factors [26, 32, 33]. The best diagnostic potential for ESCC was found for C-reactive protein [28].…”

Polymorphism of the DNA repair genes RAD51 and XRCC2 in smoking- and drinking-related laryngeal cancer in a Polish population

“…Larynx cancer is strongly associated with smoking and high alcohol consumption, as well as occupational and environmental exposure to carcinogens [1–3]. Cigarette smoke can generate reactive oxygen species, which are capable of inducing DNA double-strand breaks (DSBs) [4], the most serious DNA damage, which, if not repaired or misrepaired, may result in genomic instability, cancer transformation or cell death [5–7].…”

Protein marker profiling in different T classification in laryngeal squamous cell carcinoma

“…The dysregulation of some of these proteins reported in head and neck cancer in previous studies was consistent with our findings. For example, PCNA, CDK2, and p27 were upregulated in laryngeal carcinoma, [7][8][9] and Bcl-xL, MMP9, HMG-1, and p38 were upregulated in head and neck cancer. [10][11][12][13] However, the expression of some of the proteins differed from that described in previous publications.…”