Polymorphism of the DNA repair genes RAD51 and XRCC2 in smoking- and drinking-related laryngeal cancer in a Polish population

Abstract

IntroductionCigarette smoke and alcohol can generate reactive oxygen species, which may induce DNA double-strand breaks (DSBs), the most serious DNA lesion. In humans, DSBs are repaired mainly by non-homologous end joining and homologous recombination repair (HRR). Several polymorphisms in the DNA repair gene have been extensively studied in the association with various human cancers. In the present work we investigated the association between polymorphisms of two HRR genes, XRCC2 and RAD51, and tobacco- and alcohol-related larynx cancer in a Polish population.Material and methodsTwo polymorphisms of the XRCC2 gene, –41657C > T (rs718282) and 31479G > A (rs3218536), as well as one polymorphism of the RAD51 gene, –135G > C (rs1801320), were investigated by PCR-RFLP in 253 patients with larynx cancer and 253 age- and sex-matched non-cancer controls.ResultsAnalysis of the gene-smoking and -drinking interactions revealed a weak association between larynx cancer and the –41657C > T polymorphisms of the XRCC2 gene among the moderate alcohol drinkers. The C allele of the –135G > C polymorphism of RAD51 increased cancer risk in the smoker group. Increased risk was also found for heavy drinkers. Additionally, there were no significant differences between distributions of genotypes in subgroups assigned to different TNM stages and grades.ConclusionsThe results indicated that the –135G > C polymorphism of the RAD51 gene may be associated with smoking- and drinking-related larynx cancer in Poland.

Epidemiological Evaluation of Laryngeal Cancer Incidence in Kazakhstan for The Years 1999-2009

“…The incidence of cancer of the larynx also has statistical differences according to age and sex (Wunsch, 2004;de Souza et al, 2011), geographical features of the place of residence (Dykhno et al, 2002;Saurina et al, 2010), social class and the living standards of the studied group of population (Vassileiou et al, 2012) and a number of other external factors (Wong et al, 1993;Grant, 2012;Romanowicz-Makowska et al, 2012). In Kazakhstan, it has already been shown that there is geographical variation in incidences of breast (Bilyalova et al, 2012), esophageal (Igissinov S et al, 2012;2013), and cervical cancer , with changes dover time (Igissinov et al, 2011).…”

Association between -41657C/T single nucleotide polymorphism of DNA repair gene XRCC2 and endometrial cancer risk in Polish women

“…It has already been proven that XRCC2 -41657C/ T polymorphism is associated with increased risk of oesophageal squamous cell carcinoma (ESCC), gastric cardia adenocarcinoma (GCA) and smoking-and drinking-related laryngeal cancer [11,16]. However, the functional consequences of -41657C/T XRCC2 polymorphism in the response to different DNA damaging agents still remains unclear.…”

“…Some reports provide proof that the XRCC2 -41657C/T genotype was related to increased risk of oesophageal squamous cell carcinoma (ESCC), gastric cardia adenocarcinoma (GCA) and of smoking-and drinking-related laryngeal cancer [11,17].…”

“…Moreover, it has been proven that polymorphisms in XRCC2 may modify individual susceptibility to various types of cancer [6][7][8][9][10][11][12][13].…”

Evaluation of polymorphisms in microRNA biosynthesis genes and risk of laryngeal cancer in the Polish population

“…The molecular mechanism underlying the development of LC is still poorly understood. Many studies have shown that a defective DNA repair mechanism, impaired cell cycle and aberrant expression of the metalloproteinases might increase the risk of LC occurrence [3,4,5,6,7,8,9].…”