The prognostic significance of bromodomain PHD-finger transcription factor in colorectal carcinoma and association with vimentin and E-cadherin

Xiao, Shuai; Liu, Longfei; Lu, Xianzhou; Long, Jianwu; Zhou, Xiaojun; Fang, Min

doi:10.1007/s00432-015-1937-y

Cited by 49 publications

(40 citation statements)

References 26 publications

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“…Higher levels of mRNA of bromodomain PHD finger transcription factor was observed in 85% of tumor samples compared to controls. The results of Western blot confirmed the mRNA expression results and also 67.6% of samples showed high levels resulted from immunohistochemistry . Stomatin is a cell membrane protein whose function is unclear, but it may be involved in the regulation of ion channels and transporters.…”

Section: Resultssupporting

confidence: 69%

“…Bromodomain PHD finger transcription factor has been identified to play a possible role in transcription. Upregulation of this protein has been reported in colorectal cancer . Twenty samples of colorectal cancer were compared with controls and the levels of mRNA and protein were analyzed using RT‐PCR, Western blot analysis and immunohistochemistry.…”

Section: Resultsmentioning

confidence: 99%

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Proteomics analysis of human breast milk to assess breast cancer risk

et al. 2018

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Detection of breast cancer (BC) in young women is challenging because mammography, the most common tool for detecting BC, is not effective on the dense breast tissue characteristic of young women. In addition to the limited means for detecting their BC, young women face a transient increased risk of pregnancy-associated BC. As a consequence, reproductively active women could benefit significantly from a tool that provides them with accurate risk assessment and early detection of BC. One potential method for detection of BC is biochemical monitoring of proteins and other molecules in bodily fluids such as serum, nipple aspirate, ductal lavage, tear, urine, saliva and breast milk. Of all these fluids, only breast milk provides access to a large volume of breast tissue, in the form of exfoliated epithelial cells, and to the local breast environment, in the form of molecules in the milk. Thus, analysis of breast milk is a non-invasive method with significant potential for assessing BC risk. Here we analyzed human breast milk by mass spectrometry (MS)-based proteomics to build a biomarker signature for early detection of BC. Ten milk samples from eight women provided five paired-groups (cancer versus control) for analysis of dysregulatedproteins: two within woman comparisons (milk from a diseased breast versus a healthy breast of the same woman) and three across women comparisons (milk from a woman with cancer versus a woman without cancer). Despite a wide range in the time between milk donation and cancer diagnosis (cancer diagnosis occurred from 1 month before to 24 months after milk donation), the levels of some proteins differed significantly between cancer and control in several of the five comparison groups. These pilot data are supportive of the idea that molecular analysis of breast milk will identify proteins informative for early detection and accurate assessment of BC risk, and warrant further research. Data are available via ProteomeXchange with identifier PXD007066.

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Section: Resultssupporting

confidence: 69%

Section: Resultsmentioning

confidence: 99%

Proteomics analysis of human breast milk to assess breast cancer risk

et al. 2018

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“…During EMT, epithelial cell lose its E-cadherin expression, which contributes to intercellular adhesion and enables cells with migratory and invasive properties. Increase expression of N-cadherin, Vimentin and Fibronectin can be markers for metastasis and poor prognosis [12–14]. EMT has been demonstrated to be a good target for various therapeutic interventions [15, 16].…”

Section: Introductionmentioning

confidence: 99%

Long non-coding RNA AFAP1-AS1 facilitates tumor growth and promotes metastasis in colorectal cancer

Wang

et al. 2016

Biol Res

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Background and objectiveLong non-coding RNAs can regulate tumorigenesis of various cancers. Dys-regulation of lncRNA-AFAP1-AS1 has not been studied in colorectal carcinoma (CRC). This study was to examine the function involvement of AFAP1-AS1 in tumor growth and metastasis of CRC.MethodsRelative expression of AFAP1-AS1 in CRC tissues and CRC cells lines was determined using quantitative real-time PCR (qRT-PCR). Functional involvement of AFAP1-AS1 in tumor proliferation and metastasis was evaluated in AFAP1-AS1-specific siRNA-treated CRC cells and in CRC cell xenograft. Expression of epithelial-mesenchymal transition (EMT)-related gene expression was determined using western blot.ResultsRelative expression of AFAP1-AS1 was significantly elevated in CRC tissues and CRC HCT116 and SW480 cell lines. AFAP1-AS1 knock-down suppressed SW480 cell proliferation, colony formation, migration and invasion. Also AFAP1-AS1 knock-down inhibited tumor metastasis-associated genes expression in terms of EMT. This carcinostatic action by AFAP1-AS1 knock-down was further confirmed by suppression of tumor formation and hepatic metastasis of CRC cells in nude mice.ConclusionlncRNA-AFAP1-AS1 knock-down exhibits antitumor effect on colorectal carcinoma in respects of suppression of cell proliferation and metastasis of cancer cells.

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“…EMT further plays an important role in many cancers, such as breast cancer, lung cancer, pancreatic cancer and skin squamous cell carcinoma. It is showed that Vimentin may correlated with cancer progression and prognosis at the invasive frontier in many kinds of cancers[23]. …”

Section: Discussionmentioning

confidence: 99%

Annexin A1 can inhibit the in vitro invasive ability of nasopharyngeal carcinoma cells possibly through Annexin A1/S100A9/Vimentin interaction

et al. 2017

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Annexin A1 is a member of a large superfamily of glucocorticoid-regulated, calcium- and phospholipid-binding proteins. Our previous studies have shown that the abnormal expression of Annexin A1 is related to the occurrence and development of nasopharyngeal carcinoma (NPC). To understand the roles of Annexin A1 in the tumorigenesis of NPC, targeted proteomic analysis was performed on Annexin A1-associated proteins from NPC cells. We identified 436 proteins associated with Annexin A1, as well as two Annexin A1-interacted key proteins, S100A9 and Vimentin, which were confirmed by co-immunoprecipitation. Gene function classification revealed that the Annexin A1-associated proteins can be grouped into 21 clusters based on their molecular functions. Protein–protein interaction analysis indicated that Annexin A1 /S100A9/Vimentin interactions may be involved in the invasion and metastasis of NPC because they can form complexes in NPC cells. The down-regulation of Annexin A1 in NPC may lead to the overexpression of S100A9/Vimentin, which may increase the possibility of the invasion ability of NPC cells by adjusting the function of cytoskeleton proteins. Results suggested that the biological functions of Annexin A1 in NPC were diverse, and that Annexin A1 can inhibit the in vitro invasive ability of NPC cells through Annexin A1 /S100A9/Vimentin interaction.

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The prognostic significance of bromodomain PHD-finger transcription factor in colorectal carcinoma and association with vimentin and E-cadherin

Abstract: Our results strongly suggested that the high BPTF expression was significantly correlated with tumor progression and may be a potent prognostic marker of CRC. Moreover, BPTF expression was significantly associated with EMT markers vimentin and E-cadherin.

Cited by 49 publications

References 26 publications

Proteomics analysis of human breast milk to assess breast cancer risk

Proteomics analysis of human breast milk to assess breast cancer risk

Long non-coding RNA AFAP1-AS1 facilitates tumor growth and promotes metastasis in colorectal cancer

Annexin A1 can inhibit the in vitro invasive ability of nasopharyngeal carcinoma cells possibly through Annexin A1/S100A9/Vimentin interaction

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