The profiling and identification of the absorbed constituents and metabolites of Naoshuantong capsule in mice biofluids and brain by ultra- fast liquid chromatography coupled with quadrupole-time-of-flight tandem mass spectrometry

Luo, Lvkeng; Wu, Shuling; Zhu, Xiaoxiao; Su, Weiwei

doi:10.1016/j.jchromb.2019.121791

Cited by 12 publications

(18 citation statements)

References 26 publications

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“…Both negative and positive mode was set for compounds ionization. The conditions of MS detector were as described elsewhere [8]. The chromatographic separation was carried out on a Shimadzu UFLC XR instrument (Shimadzu, Kyoto, Japan) and a Phenomenex Kinetex column (2.1 mm × 100 mm, 2.6 µm, Phenomenex, CA, USA).…”

Section: Standardization Of Nstcmentioning

confidence: 99%

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The study of neuroprotective effects and underlying mechanism of Naoshuantong capsule on ischemia stroke mice

Luo¹,

Wu²,

Chen³

et al. 2020

Preprint

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Background Naoshuantong capsule (NSTC) is an oral Chinese medicine formula composed of Typhae Pollen (TP), Radix Paeoniae Rubra (PR), Curcumae Radix (CR), Gastrodiae Rhizoma (GR) and Radix Rhapontici (RR). It has been widely used at the acute and recovery stage of ischemic stroke since 2001. Comparing with its wide clinical application, there are only few studies emphasize on investigating its pharmacological effects. Methods To more generally elucidate the underlying mechanisms in this study, we identified active ingredients in NSTC by a network pharmacology approach based on transcriptomics analysis and pharmacological experiments. Modified neurological severity scores (mNSS) and morphometric analysis using Nissl staining were employed to evaluate the neuroprotective effects of NSTC on ischemia stroke in mice. Results The results showed that NSTC had preventive and protective effects on ischemia stroke, featuring repair of brain tissue during the sub-acute stage of stroke. This may attribute to the underlying mechanisms including anti-inflammatory, antioxidant, and anti-apoptotic activities, as well as an attenuation of excitatory amino acids (EAAs) toxicity of the active ingredients, especially the most active apigenin, from NSTC. Specifically, naringenin, calycosin, gastrodin, caffeic acid, paeoniflorin, and β -elemene seem to be also pharmacological active substances responsible for the anti-inflammatory effects. Meanwhile, 13-hydroxygemone, gastrodin, and p-hydroxybenzyl alcohol contributed to the attenuation of EAAs toxicity Furthermore, apigenin, naringenin, calycosin, gastrodin, and β-elemene accelerated the repair of brain ischemic tissue by up-regulating the expression of TGF-β1 levels. Conclusions The present study identifies the active ingredients of NSTC and illustrates the underlying mechanism using a combination of network pharmacology, transcriptomics analysis, and pharmacological experiments.

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Section: Standardization Of Nstcmentioning

confidence: 99%

“…Nineteen transition components of NSTC (9 prototype components and 10 metabolites) that had been detected in the brain in the previous study [8] were used for screening potential targets. Details of their chemical structures and information were shown in supplementary material Fig.…”

Section: Data Preparation and Target Predictionmentioning

confidence: 99%

The study of neuroprotective effects and underlying mechanism of Naoshuantong capsule on ischemia stroke mice

Luo¹,

Wu²,

Chen³

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…The conditions of MS detector were as described elsewhere [8]. The chromatographic separation was carried out on a Shimadzu UFLC XR instrument (Shimadzu, Kyoto, Japan) and a Phenomenex Kinetex column (2.1mm ×100mm, 2.6μm, Phenomenex, CA, USA).…”

Section: Standardization Of Nstcmentioning

confidence: 99%

Section: Data Preparation and Target Predictionmentioning

confidence: 99%

“…Molecular evidences show that NSTC exerts an inhibitory effect on neuronal apoptosis caused by stroke [5], with improved blood rheology and energy metabolism in rats [6]. Although the constituents [7] and metabolic pro le [8] of NSTC have been fully investigated, not much research has been done in pharmacological mechanism of NSTC, which is an emerging bottleneck restraining its clinical application.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The study of neuroprotective effects and underlying mechanism of Naoshuantong capsule on ischemia stroke mice

Luo¹,

Wu²,

Chen³

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

Background: Naoshuantong capsule (NSTC) is an oral Chinese medicine formula composed of Typhae Pollen, Radix Paeoniae Rubra, Curcumae Radix, Gastrodiae Rhizoma and Radix Rhapontici. It has been widely used at the acute and recovery stage of ischemic stroke since 2001. Comparing with its wide clinical application, there are only few studies emphasize on investigating its pharmacological effects.Methods:To more generally elucidate the underlying mechanisms in this study, we identified active ingredients in NSTC by a network pharmacology approach based on transcriptomics analysis and pharmacological experiments. Modified neurological severity scores and morphometric analysis using Nissl staining were employed to evaluate the neuroprotective effects of NSTC on ischemia stroke in mice.Results: The results showed that NSTC had preventive and protective effects on ischemia stroke, featuring repair of brain tissue during the sub-acute stage of stroke. This may attribute to the underlying mechanisms including anti-inflammatory, antioxidant, and anti-apoptotic activities, as well as an attenuation of excitatory amino acids (EAAs) toxicity of the active ingredients, especially the most active apigenin, from NSTC. Specifically, naringenin, calycosin, gastrodin, caffeic acid, paeoniflorin, and β -elemene seem to be also pharmacological active substances responsible for the anti-inflammatory effects. Meanwhile, 13-hydroxygemone, gastrodin, and p-hydroxybenzyl alcohol contributed to the attenuation of EAAs toxicity Furthermore, apigenin, naringenin, calycosin, gastrodin, and β-elemene accelerated the repair of brain ischemic tissue by up-regulating the expression of TGF-β1 levels.Conclusions: The present study identifies the active ingredients of NSTC and illustrates the underlying mechanism using a combination of network pharmacology, transcriptomics analysis, and pharmacological experiments.

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Metabolite identification of iridin in rats by using UHPLC-MS/MS and pharmacokinetic study of its metabolite irigenin

Wang

et al. 2021

Journal of Chromatography B

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The profiling and identification of the absorbed constituents and metabolites of Naoshuantong capsule in mice biofluids and brain by ultra- fast liquid chromatography coupled with quadrupole-time-of-flight tandem mass spectrometry

Cited by 12 publications

References 26 publications

The study of neuroprotective effects and underlying mechanism of Naoshuantong capsule on ischemia stroke mice

The study of neuroprotective effects and underlying mechanism of Naoshuantong capsule on ischemia stroke mice

The study of neuroprotective effects and underlying mechanism of Naoshuantong capsule on ischemia stroke mice

Metabolite identification of iridin in rats by using UHPLC-MS/MS and pharmacokinetic study of its metabolite irigenin

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