The Profile of MicroRNA Expression in Bone Marrow in Non-Hodgkin’s Lymphomas

Abstract

Non-Hodgkin’s lymphomas (NHLs) are a heterogeneous group of malignant lymphomas that can occur in both lymph nodes and extranodal sites. Bone marrow (BM) is the most common site of extranodal involvement in NHL. The objective of this study is to determine the unique profile of miRNA expression in BM affected by NHL, with the possibility of a differential diagnosis of NHL from reactive BM changes and acute leukemia (AL). A total of 180 cytological samples were obtained by sternal puncture and aspiration biopsy of BM from the posterior iliac spine. All the cases were patients before treatment initiation. The study groups were NHL cases (n = 59) and AL cases (acute lymphoblastic leukemia (n = 25) and acute myeloid leukemia (n = 49)); the control group consisted of patients with non-cancerous blood diseases (NCBDs) (n = 48). We demonstrated that expression levels of miRNA-124, miRNA-221, and miRNA-15a are statistically significantly downregulated, while the expression level of let-7a is statistically significantly upregulated more than 2-fold in BM in NHL compared to those in AL and NCBD. ROC analysis revealed that let-7a/miRNA-124 is a highly sensitive and specific biomarker for a differential diagnosis of BM changes in NHL from those in AL and NCBD. Therefore, we conclude that analysis of miRNA expression levels may be a promising tool for early diagnosis of NHL.

MicroRNA Expression Profile in Bone Marrow and Lymph Nodes in B-Cell Lymphomas

“…We added miRNA-182-5p to the analysis to assess the contribution of the miRNA-183/96/182 cluster to the pathogenesis of lymphomas. The geometric mean of Ct values of three miRNAs (-378-3p, -191-5p, and -103a-3p), which were selected based on our previous data [47], was used for normalization. The sequences of oligonucleotides for reverse transcription and PCR are presented in Table S2.…”