The production of polyhydroxyalkanoates in recombinant Escherichia coli

Li, Rui; Zhang, Hanxing; Qi, Qingsheng

doi:10.1016/j.biortech.2006.09.014

Cited by 151 publications

(68 citation statements)

References 77 publications

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“…The PHB production rates were also high in the initial operation and decreased after 188 h, but PHB production continued at a rate of 5 mgL 1 h 1 even at 259 h (at the 8th cycle). The PHB content (<87%) was higher than that produced from the 9 filtered fermentation liquid (Du and Yu, 2002) and comparable to that obtained from pure feedstock (Li et al, 2007;Lee, 1996). concentrations and PHB content in the PHB production reactor (A).…”

Section: Resultssupporting

confidence: 51%

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Effect of feeding regimens on polyhydroxybutyrate production from food wastes by Cupriavidus necator

Hafuka

Sakaida

Satoh

et al. 2011

Bioresource Technology

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List of abbreviations: PHA, polyhydroxyalkanoate; PHB, polyhydroxybutyrate; DO, dissolved oxygen; DCW, dry cell weight; COD, chemical oxygen demand; VFA, volatile fatty acid; D-COD, dissolved chemical oxygen demand; GC, gas chromatography; PHB-PHV, poly(3-hydroxybutyrate-co-3-hydroxyvalerate) 2 ABSTRACT We investigated the effects of different feeding regimens (1-pulse, stepwise, and continuous) of fermented food-waste liquid on polyhydroxybutyrate (PHB) production.The fermentation liquid was filtered with a membrane filter (pore size, 0.45 μm) to remove anaerobic microorganisms and solids and used as a carbon source for Cupriavidus necator. One-pulse feeding yielded the highest cell concentration of C.necator. However, the PHB concentration was higher in the stepwise-and continuous-feeding regimens. Therefore, the continuous-feeding regimen was used for continuous PHB production. PHB could be produced over 259 h (8 draw-fill cycles) with a maximal PHB content of 87%, but the PHB concentration and content decreased with an increase in the operation time.

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Section: Resultssupporting

confidence: 51%

“…In contrast, PHA production by pure cultures yields a very high PHA content (ca. 80%) (Li et al, 2007;Lee, 1996). Hence, these 2 different processes should be coupled in a more cost-effective method.…”

Section: Introductionmentioning

confidence: 99%

Effect of feeding regimens on polyhydroxybutyrate production from food wastes by Cupriavidus necator

Hafuka

Sakaida

Satoh

et al. 2011

Bioresource Technology

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“…Recently, PHAs have attracted considerable attention due to their potential use as biodegradable thermoplastics and sources of chiral monomers (34,44,45). Thus, research is currently being performed to improve productivity, to reduce production costs and, more importantly, to produce specific functionalized PHAs (46)(47)(48). Therefore, the aim of the present study is to review the current advances and progress in biosynthesis, accumulation, extraction, chemical and physical properties, biocompatibility, degradation and potential applications of bacterial thermoplastic PHAs.…”

Section: Introductionmentioning

confidence: 99%

Bacterial polyhydroxyalkanoates-eco-friendly next generation plastic: Production, biocompatibility, biodegradation, physical properties and applications

Muhammadi

Shabina

Afzal

et al. 2015

Green Chemistry Letters and Reviews

266

139

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Polyhydroxyalkanoates (PHAs) are intracellular aliphatic polyesters synthesized as energy reserves, in the form of water-insoluble, nano-sized discrete and optically dense granules in cytoplasm by a diverse bacteria and some archae under conditions of limiting nutrients in the presence of excess carbon source. Bacteria synthesize different PHAs from coenzyme A thioesters of respective hydroxyalkanoic acid, and degrade intracellularly for reuse and extracellularly in natural environments by other microorganisms. In vivo, PHAs exist as amorphous mobile liquid and water-insoluble inclusions but in vitro, exhibit material and mechanical properties, ranging from stiff and brittle crystalline to elastomeric and molding, similar to petrochemical thermoplastics. Further, they are hydrophobic, isotactic, biocompatible and exhibit piezoelectric properties. But as commodity plastics their applications are limited by high production cost, low yield, in vivo degradation, complexity of technology and difficulty of extraction. Therefore, to replace the conventional plastic with PHAs, it is prerequisite to standardize the PHA production systems.

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“…The application of the method is demonstrated by the quantitative analysis of the metabolites produced by the enzymatic degradation of a microbial polyester, poly(3-hydroxybutyrate), PHB [43][44][45]. The enzyme used for catalysis originated from the strain Bacillus Megaterium.…”

Section: Introductionmentioning

confidence: 99%

The novel technique of vapor pressure analysis to monitor the enzymatic degradation of PHB by HPLC chromatography

Polyák

Rácz

Rózsa

et al. 2017

Analytical Biochemistry

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The production of polyhydroxyalkanoates in recombinant Escherichia coli

Cited by 151 publications

References 77 publications

Effect of feeding regimens on polyhydroxybutyrate production from food wastes by Cupriavidus necator

Effect of feeding regimens on polyhydroxybutyrate production from food wastes by Cupriavidus necator

Bacterial polyhydroxyalkanoates-eco-friendly next generation plastic: Production, biocompatibility, biodegradation, physical properties and applications

The novel technique of vapor pressure analysis to monitor the enzymatic degradation of PHB by HPLC chromatography

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