The novel technique of vapor pressure analysis to monitor the enzymatic degradation of PHB by HPLC chromatography

Polyák, Péter; Rácz, Piroska; Rózsa, P.; Nagy, Gergely; Vértessy, Beáta G.; Pukánszky, Béla

doi:10.1016/j.ab.2017.01.004

Cited by 5 publications

(1 citation statement)

References 49 publications

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“…[ 204 ] The 3‐hydroxybutyrate monomer generated during the enzymatic degradation of poly(3‐hydroxybutyrate) has been quantified using a diode array detector in the HPLC method. [ 205 ]…”

Section: Identification Quantification and Characterization Of Phamentioning

confidence: 99%

Process optimization, metabolic engineering interventions and commercialization of microbial polyhydroxyalkanoates production – A state‐of‐the art review

Lhamo

Behera

Mahanty

2021

Biotechnology Journal

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Background Microbial polyhydroxyalkanoates (PHAs) produced using renewable resources could be the best alternative for conventional plastics. Despite their incredible potential, commercial production of PHAs remains very low. Nevertheless, sincere attempts have been made by researchers to improve the yield and economic viability of PHA production by utilizing low‐cost agricultural or industrial wastes. In this context, the use of efficient microbial culture or consortia, adoption of experimental design to trace ideal growth conditions, nutritional requirements, and intervention of metabolic engineering tools have gained significant attention. Purpose and scope This review has been structured to highlight the important microbial sources for PHA production, use of conventional and non‐conventional substrates, product optimization using experimental design, metabolic engineering strategies, and global players in the commercialization of PHA in the past two decades. The challenges about PHA recovery and analysis have also been discussed which possess indirect hurdle while expanding the horizon of PHA‐based bioplastics. Summary Selection of appropriate microorganism and substrate plays a vital role in improving the productivity and characteristics of PHAs. Experimental design‐based bioprocess, use of metabolic engineering tools, and optimal product recovery techniques are invaluable in this dimension. Conclusion Optimization strategies, which are being explored in isolation, need to be logically integrated for the successful commercialization of microbial PHAs.

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Section: Identification Quantification and Characterization Of Phamentioning

confidence: 99%

Process optimization, metabolic engineering interventions and commercialization of microbial polyhydroxyalkanoates production – A state‐of‐the art review

Lhamo

Behera

Mahanty

2021

Biotechnology Journal

View full text Add to dashboard Cite

show abstract

Polyhydroxybutyrate (PHB): A Standout Biopolymer for Environmental Sustainability

Rajan¹,

Thomas²,

Gopanna³

et al. 2017

Handbook of Ecomaterials

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Polyhydroxybutyrate (PHB): A Standout Biopolymer for Environmental Sustainability

Rajan

Thomas

Gopanna

et al. 2018

Handbook of Ecomaterials

View full text Add to dashboard Cite

The novel technique of vapor pressure analysis to monitor the enzymatic degradation of PHB by HPLC chromatography

Cited by 5 publications

References 49 publications

Process optimization, metabolic engineering interventions and commercialization of microbial polyhydroxyalkanoates production – A state‐of‐the art review

Process optimization, metabolic engineering interventions and commercialization of microbial polyhydroxyalkanoates production – A state‐of‐the art review

Polyhydroxybutyrate (PHB): A Standout Biopolymer for Environmental Sustainability

Polyhydroxybutyrate (PHB): A Standout Biopolymer for Environmental Sustainability

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