The Production of Nanoparticles Chitosan from Crustaceans Shell Using the Top-down Method

Abstract

Research on the manufacture of nanoparticles of chitosan through top-down method has a limited publication. Most of the research on chitosan nanoparticles was conducted by ionic gelation and other methods. Therefore, this study aimed to investigate the characteristics of nanoparticles chitosan through top-down method. The rprocedure was composed using High Energy Milling (HEM) with different sizes of ball and milling time. The responses observed from this research were particle sizes, yield, viscosity and zeta potential. The results showed that milling time significantly affected the particle size. Milling time between 3 to 5 hour produce the particle size close to 200 nm. Milling duration for 5 hours with a small ball size produced shrimp chitosan nanoparticles with the value of 248.28 nm particle size, zeta potential mV of 44.92, yield of 96% and 104.88 cP viscosity. The nanoparticle produced was categorized as relatively stable because its zeta potential values were around ± 30 mV.

Application of Bentonite Clay, Date Pit, and Chitosan Nanoparticles as Promising Adsorbents to Sequester Toxic Lead and Cadmium from Milk

“…Chitosan nanoparticles (Cs-NP), colloidal solid particles ranging from 1 to 1000 nm, have piqued the interest of researchers due to their nano size, wide surface area, biodegradability, and remarkable biocompatibility [21]. These characteristics contributed to their high adsorption potential and reactivity, which are both beneficial for extracting heavy metal ions [22].…”

Chitosan-based Biogenic Nanoparticles for Wastewater Remediation: Synthesis, Characterization, and Applications - A Review

“…Several specific techniques are employed for the fabrication of chitosan-based biogenic nanoparticles, each offering distinct advantages in terms of control over nanoparticle properties and scalability. Because they are more stable and have a higher adsorption capacity and better transport capability, chitosan nanoparticles enhance the benefits of chitosan 29 . The past several decades has seen the use of a variety of techniques, including ionic gelation, microemulsions, emulsification, cross-linking, and polymerization, to generate chitosan nanoparticles.…”

“…The chitosan particle size and molecular structure produced by this approach vary depending on some changes in the procedure. 300 nm to 500 nm is typically the range of findings from such procedures 29 . The synthesis of nanoparticles via biogenesis is favoured over chemical or physical methods because it is a less time-consuming, safe, environmentally benign, economically and energetically feasible process that maximises the redox potential of metabolites produced by biological entities to transform macromolecules into nano form 30 .…”

Microscopic Image Segmentation and Morphological Characterization of Novel Chitosan/Silica Nanoparticle/Nisin Films Using Antimicrobial Technique for Blueberry Preservation

“…The algorithm processed the image of cluster 2 (holes) a median filtering stage, of 5 × 5 pixels mask, eliminates the small pixels (salt and pepper) and a binarization step using OTSU method to adapt the threshold according to the image [51] is necessary. Finally, the regions (holes) were extracted.…”

“…ζ-potential of the novel films ranged from 8.92 mV in Chitosan to 45.9 mV in Chitosan/Silica Nanoparticle/N films, respectively, Figure 5a. Syamdidi et al, [51] reported that nanoparticles with a ζ-potential above 40 mV can present a stable suspension as the surface loads may prevent aggregations among particles. The reasonably stable ζ-potential values could be due to the presence of nisin that take a part in reducing the tension between solid-liquid surfaces and blocks the aggregations between particles.…”