Agaricus bisporus is an edible fungus with a limited shelf life due to high moisture loss, browning, respiration, self-dissolve, lack of physical protection, rotting, and microbial attack. Mushrooms have been coated with nisin, nano-silica, and chitosan films in order to extend the shelf life, preserve quality and oxidation activities. The results showed that treating the mushrooms with chitosan and nano-silica (CH-AN-SILICA) increased superoxide dismutase activity (SOD—6.53 U kg−1), total phenolic content (TPC—0.39 g kg−1), and malondialdehyde content (MDA—1.63 µmol kg−1). CH-AN-SILICA treatment exhibited the highest scavenging against 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radicals. While, CH-AN-SILICA with the addition of nisin as an antimicrobial agent preserved almost the reactive oxygen species such as hydroxyl radicals (OH—0.33 µmol g−1), superoxide anion (O2•−—0.271 mmol s−1 kg−1), and hydrogen peroxide (H2O2—21.54 µmol g−1). Besides, both CH-AN-SILICA and CH-AN-SILICA/N enhanced the catalase (CAT) activity and reduced the respiration rate. The results indicated that the combination of nisin, nano-silica, and chitosan coating films was effective in providing a longer storage life with acceptable quality in mushrooms.
This study was designed to preparecarboxyl-functionalized poly (N-isopropylacrylamide) PNIPAM microgels having excellent catalytic properties.Recently, researchers are trying to fabricate cost effective and efficient hybrid catalytic materials for the synthesis of nitrogenous compounds along with enhanced optical properties. For the same motive, synthesis of carboxyl-functionalized PNIPAM microgels was performed by using polymerization of soap-free emulsion of N-isopropyl acrylamide, which is NIPAM along with acrylic acid (AA). The thiol group was introduced through the imide bond mediated by carbodiimide, between carboxyl-functionalized microgels through carboxyl group and aminoethanethiol (AET). Copper, Palladium and Cu/Pd nanoparticles were incorporated successfully into thiol-functionalized PNIPAM microgels through metals thiol linkage. The synthesized microgels and hybrid encompassing metallic nanoparticles were characterized in detail by using Transmission electron microscopy (TEM), Scanning electron microscopy (SEM), X-ray diffraction (XRD), X-ray photoelectron (XPS) and Fourier transformed infrared spectroscopy for structural interpretation. The thermal properties of the pure and hybrid microgels were inspected by TG analysis. The prepared nanocomposites PNIPAM-Cu, PNIPAM-Pd and PNIPAM-Cu/Pd exhibited decent catalytic properties for the degradation of 4-Nitrophenol and methylene blue, but the bimetallic Cu/Pd have remarkable catalytic properties. The catalytic reaction followed pseudo-first-order reaction with rate constants 0.223 min−1, 0.173 min−1 for 4-Nitrophenol and methylene blue in that order. In this study,we were able to establish that Cu/Pd hybrid is an efficient catalyst for 4-Nitrophenol and methylene blue as compared to its atomic analogue.
White button mushrooms are greatly high perishable and can deteriorate within a few days after harvesting due to physicomechanical damage, respiration, microbial growth of the delicate epidermal structure. For that reason, the present research work was applied to evaluate the effect of chitosan combination with nano-coating treatments on physicochemical parameters and microbial populations on button mushrooms at chilling storage. Nano coating with the addition of nisin 1% (CHSSN/M) established the minimum value for weight loss 12.18%, maintained firmness 11.55 N, and color index profile. Moreover, O2% rate of (CHSSN/M) mushrooms was the lowest at 1.78%; while the highest rate was reported for CO2 24.88% compared to the untreated samples (Control/M) on day 12. Both pH and total soluble solid concentrations increased during storage. Results reported that the (CHSS/M) mushroom significantly (P < 0.05) reduced polyphenol oxidase activity (24.31 U mg−1 Protein) compared with (Control/M) mushrooms that increased faster than the treated samples. (CHSSN/M) treatment was the most efficient in the reduction of yeast and mold, aerobic plate microorganisms (5.27–5.10 log CFU/g), respectively. The results established that nano-coating film might delay the aging degree and accompany by marked prolongation of postharvest mushroom freshness.
In the current work, the characterization of novel chitosan/silica nanoparticle/nisin films with the addition of nisin as an antimicrobial technique for blueberry preservation during storage is investigated. Chitosan/Silica Nanoparticle/N (CH-SN-N) films presented a stable suspension as the surface loads (45.9 mV) and the distribution was considered broad (0.62). The result shows that the pH value was increased gradually with the addition of nisin to 4.12, while the turbidity was the highest at 0.39. The content of the insoluble matter and contact angle were the highest for the Chitosan/Silica Nanoparticle (CH-SN) film at 5.68%. The use of nano-materials in chitosan films decreased the material ductility, reduced the tensile strength and elongation-at-break of the membrane. The coated blueberries with Chitosan/Silica Nanoparticle/N films reported the lowest microbial contamination counts at 2.82 log CFU/g followed by Chitosan/Silica Nanoparticle at 3.73 and 3.58 log CFU/g for the aerobic bacteria, molds, and yeasts population, respectively. It was observed that (CH) film extracted 94 regions with an average size of 449.10, at the same time (CH-SN) film extracted 169 regions with an average size of 130.53. The (CH-SN-N) film presented the best result at 5.19%. It could be observed that the size of the total region of the fruit for the (CH) case was the smallest (1663 pixels), which implied that the fruit lost moisture content. As a conclusion, (CH-SN-N) film is recommended for blueberry preservation to prolong the shelf-life during storage.
Chitosan coating plus silicon dioxide nanoparticles and nisin were applied on fresh blueberry samples in order to find out safety packaging assay during the post-harvest process. Studies were performed in-vitro for fruit quality as physicochemical parameters and oxidation, while microbiological analyses as molds/yeast and mesophilics populations were examined in-vivo. The selected silicon dioxide nanoparticles 1% and nisin 1%, were added into a chitosan solution, which resulted in four groups of coated blueberries. After storage at ambient temperature, fruits were examined for two, four, six, and eight days. It was noticed that the hardness, chewiness, and cohesiveness of all blueberry samples were increased during the storage. Chitosan-nano-silicon dioxide (CHN-Nano) and (CHN-N-Nano) with the addition of nisin helped to control shrinking (38.52%) and decay rates (8.61%). Moreover, (CHN-N-Nano) reported the lowest L* values (10.54) for the color index, and inhibited the microbial populations (3.60 and 2.73 log CFU/g) for molds/yeast and mesophilics, respectively. (CHN-Nano) reported the lowest value for ph (2.61) and the highest for anthocyanin content (75.19 cyanidin-3-glucoside mg/100 g). The chitosan coating substantially maintained Vitamin C (7.34 mg/100 g) and polyphenoloxidase (PPO) (558.03 U min−1·g−1). The results suggest that nano-material with chitosan film coatings that contained nisin were effective for fresh blueberry preservation under ambient temperature.
Agaricus Bisporus is an edible button mushroom that is highly perishable with an extremely short shelf-life at ambient temperature. This work aims to evaluate some antioxidant activities, oxidation enzymes, and determine postharvest qualities of nano-coated mushrooms with the combination of chitosan (CHN) component during storage at 4 °C for (0, 3, 6, 9, and 12 days). Silica/CHN Film strongly delayed the mushroom respiratory spike onset and blocked carbon dioxide passage from inside to the outside, while Titanium/CHN Film (0.035 mmol s−1 kg−1) established the lowest O2 production rate and thiobarbituric acid reactive substances production (6.21 nmol g−1). Silica/CHN Film was mainly effectual for the polyphenol contents (0.39 g kg−1) and antioxidant activities (78.14% and 71.09%) for DPPH and ABTS+ radical scavenging activities, respectively. The results reported that Silica/CHN Film induced the highest (catalase and ascorbate peroxidase) activities, while Titanium/CHN Film recorded the highest (peroxidase and superoxide dismutase) activities of antioxidant enzymes. Besides, Titanium/CHN Film preserved relatively lower contents of hydrogen peroxide (22.40 µmol g−1) and hydroxyl radical (0.16 µmol g−1). In a word, nano-materials used in coating films such as titanium or even silica with the combination of CHN can directly reduce the cell degradation, oxidation processes and enhance the harvested horticultural crops.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
334 Leonard St
Brooklyn, NY 11211
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.