The Production of CINC/gro, a Member of the Interleukin-8 Family, in Rat Anterior Pituitary Gland

Koike, Koji; Sakamoto, Yoshiki; Sawada, Takuya; Ohmichi, Masahide; Kanda, Yuki; Nohara, A.; Hirota, Kenji; Kiyama, Hiroshi; Miyake, Akira

doi:10.1006/bbrc.1994.1907

Cited by 24 publications

(6 citation statements)

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“…No direct effects of IL-8 on pituitary hormone secretion have been reported, however, the cytokineinduced neutrophil chemoattractant, CINC/gro, a member of the IL-8 family, has been identified immunocytochemically and by Western blot analysis in the normal rat anterior pituitary (14). This cytokine has been shown to stimulate the production of prolactin and to inhibit secretion of luteinizing hormone and folliclestimulating hormone by rat anterior pituitary cells (15).…”

Section: Discussionmentioning

confidence: 99%

IL-8 mRNA expression by in situ hybridisation in human pituitary adenomas

Suliman

Royds²,

Baxter³

et al. 1999

European Journal of Endocrinology

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Several cytokines have been shown to be expressed in normal and adenomatous pituitary tissue. Recently, interleukin-8 (IL-8) mRNA was identified by reverse transcription (RT)-PCR in each of a series of 17 pituitary tumours examined. We have investigated further the presence of IL-8 mRNA, using in situ hybridisation in two normal human anterior pituitary specimens and 25 human pituitary adenomas. IL-8 mRNA was not identified in either of the two normal pituitary specimens. Only three of the 25 adenomas were positive for IL-8 mRNA. In these three tumours, which included two null cell adenomas and one gonadotrophinoma, the majority of tumour cells (>90%) were positive for IL-8 mRNA. The remaining 22 adenomas were completely negative. There was no difference in tumour size or type between the IL-8 positive and the IL-8 negative tumours, and immunocytochemistry for von Willebrandt factor showed that the two groups were also similar in their degree of vascularisation.In conclusion, IL-8 mRNA was found in 12% of pituitary adenomas studied and was histologically identified within the tumour cells. In situ hybridisation is a more appropriate technique for assessing cytokine mRNA production by human pituitary tumours because RT-PCR may be too sensitive, identifying very small, possibly pathologically insignificant, quantities of mRNA that could be produced by supporting cells such as fibroblasts, endothelial cells or macrophages. European Journal of Endocrinology 140 155-158

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Section: Discussionmentioning

confidence: 99%

IL-8 mRNA expression by in situ hybridisation in human pituitary adenomas

Suliman

Royds²,

Baxter³

et al. 1999

European Journal of Endocrinology

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“…Importantly, a sustained CINC secretion was observed in a subpopulation of cultured pituitary [121] and FS cells [122]. Thus, an autocrine/paracrine intrapituitary regulation may occur via the secretion of CINC/GRO by pituitary cells that, in turn, may modulate the activity of CXCR2-expressing cells.…”

Section: Autocrine/paracrine Functions Of Chemokine In Normal Pituitarymentioning

confidence: 95%

The Chemokine SDF1/CXCL12: A Novel Autocrine/Paracrine Factor Involved In Pituitary Adenoma Development

Barbieri¹

2011

TONEUROEJ

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Abstract:The relevance of the chemokine/chemokine receptor system has broaden to several tissues. Besides their physiologic role as chemotactic cytokines in immune surveillance, it is now clear that chemokines are key players in several pathological processes such as inflammation, infection, and cancer. In particular the altered expression of SDF1/CXCL12 and its receptor CXCR4 strongly affects cancer cell proliferation, recruitment of immunosuppressive cells, neovascularization and metastasization. CXCL12 and CXCR4 are widely expressed in central nervous system cells including hypotalamus and pituitary gland. Increasing evidence supports now the hypothesis that CXCL12 acts as a neuromodulatory activity on the hypothalamus/pituitary axis and is a regulatory molecule of pituitary function. Moreover, the differential expression of CXCL12 and CXCR4 in normal and tumor pituitary adenoma cells suggests their involvement in pituitary tumor development through autocrine/paracrine mechanisms leading to adenoma cell proliferation and hormone hypersecretion. Future studies focusing the pathways sustained by chemokines may help identify the tumor-initiating events and accordingly possible strategies may be developed targeting these pathways in human pituitary tumors.

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“…There is considerable evidence that CINC is produced in the central nervous system (10,11), although CINC was originally identified as an essential mediator in the inflammatory system. In addition, it has been shown that CINC stimulates the secretion of prolactin, GH, and ACTH but suppresses the secretion of LH and FSH from rat anterior pituitary cells (12). These reports suggest that CINC may be involved in regulating the hypothalamuspituitary-adrenal (HPA) axis, contributing to the neuroendocrine interface.…”

mentioning

confidence: 97%

Involvement of Cytokine-Induced Neutrophil Chemoattractant in Hypothalamic Thermoregulation of Luteinizing Hormone-Releasing Hormone

et al. 2008

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We demonstrated in a previous study that serum IL-8 concentrations were significantly higher in women with hot flashes than without hot flashes. To clarify the role of IL-8 in the pathoetiology of menopausal hot flashes, we examined the effect of rat cytokine-induced neutrophil chemoattractant (CINC), a member of the IL-8 family, on thermoregulation using ovariectomized (OVX) rats treated with intracerebroventricular (i.c.v.) injection of LHRH agonist (LHRHa) as a model of hot flashes. We found that: 1) expression of CINC mRNA was increased around the periventricular area in the hypothalamus at 1 h, and the serum CINC concentration was increased at 2 h after i.c.v. injection of LHRHa; 2) the increase in serum CINC concentration in hypophysectomized rats was significantly lower than that in sham-operated rats; 3) i.c.v. but not iv injection of CINC elevated the rectal temperature of OVX rats; 4) i.c.v. injection of LHRHa into OVX rats produced a rapid rise (maximal increase: 10-25 min) in tail skin temperature, and the elevation was augmented by injection of an anti-CINC antibody; and 5) changes in serum CINC concentration and skin temperature after i.c.v. injection of LHRHa were reversed by replacement of estradiol. In conclusion, the production of CINC in the hypothalamus due to LHRHa injection in OVX rats was increased after elevation of skin temperature, suggesting that CINC plays a key role in the homeostasis of body temperature. Disturbance of the thermoregulatory mechanism involving LHRH and CINC may be related to the pathoetiology of hot flashes.

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The Production of CINC/gro, a Member of the Interleukin-8 Family, in Rat Anterior Pituitary Gland

Cited by 24 publications

References 0 publications

IL-8 mRNA expression by in situ hybridisation in human pituitary adenomas

IL-8 mRNA expression by in situ hybridisation in human pituitary adenomas

The Chemokine SDF1/CXCL12: A Novel Autocrine/Paracrine Factor Involved In Pituitary Adenoma Development

Involvement of Cytokine-Induced Neutrophil Chemoattractant in Hypothalamic Thermoregulation of Luteinizing Hormone-Releasing Hormone

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