The production of a human monoclonal antibody defining a split of HLA‐DRw13 (DRw13b)

Sutton, Paul M.; Ting, A.; Morris, Peter J.; Bunce, Michael

doi:10.1111/j.1399-0039.1990.tb01808.x

Cited by 6 publications

(2 citation statements)

References 8 publications

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“…In each experiment 5000 cells were counted and the percentage of the positive cells was calculated. *1, Bunce et al 1990;2, Sachs et al 1986;3, Cayrol et al 1992;4,5, Bodmer et al 1984;6, Madox & Bodmer 1989;7, Madrigal et al 1989;8, Sadler et al 1993;9, Koning et al 1986; 10, Teale, personal communication), 11, Davies et al 1987Davies et al . © 1997 Blackwell Science Ltd, European Journal of Immunogenetics 24, 211-223…”

Section: Immunofluorescence and Flow Cytometry (Facs) Analysismentioning

confidence: 99%

“…One anti-HLA mAb (7.3.19.1), found in previous studies to be non-reactive with bovine lymphocytes, was used as negative reference. The anti-HLA class II mAb were derived from mice immunized with human lymphocytes (Bodmer et al, 1984;Koning et al, 1986;Sachs et al, 1986;Madrigal et al, 1989;Bunce et al, 1990;Cayrol et al, 1992;Sadler et al, 1993). The reagents used in the analysis were derived from the stock used in HLA-class II typing.…”

Section: Monoclonal Antibodiesmentioning

confidence: 99%

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The Specificity of Anti‐hla Class Ii Monoclonal Antibodies in Cattle

Nilsson

Marsh

Joosten

et al. 1997

European Journal of Immunogenetics

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At the Eleventh International HLA Histocompatibility Workshop, numerous anti-HLA class II monoclonal antibodies (mAb) were tested. For several of the polymorphic mAb, one epitope for binding has been mapped within the antigen-binding site of the class II molecules. Screening of the available bovine DRB3 and DQB exon 2 sequences revealed that some of the key amino acid (AA) motifs of these epitopes were present in cattle as well, and the question was raised whether this sharing of key AA motifs might cause interspecies cross-reactivity. Eight polymorphic anti-HLA class II mAb (seven anti-HLA DRB1 and one anti-HLA DQB) were selected for analysis of their reactivity towards bovine lymphocytes. In addition, the monomorphic anti-HLA class II mAb, 7.5.10.1, was selected for analysis, as this mAb was described to detect class II polymorphism in cattle. Flow cytometry and lymphocyte microcytotoxicity testing revealed that five of the polymorphic anti-HLA mAb were reactive with bovine lymphocytes. Furthermore, the anti-bovine reactivity of 7.5.10.1 was confirmed. These findings were supported by biochemical analysis. The anti-bovine reaction of the anti-HLA mAb did not correspond with the expected reaction, which was based on the presence of the AA, postulated to be responsible for recognition. Therefore, we suggest that the patterns of reactivity of the anti-HLA mAb are not always determined by one epitope.

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Section: Immunofluorescence and Flow Cytometry (Facs) Analysismentioning

confidence: 99%

Section: Monoclonal Antibodiesmentioning

confidence: 99%

The Specificity of Anti‐hla Class Ii Monoclonal Antibodies in Cattle

Nilsson

Marsh

Joosten

et al. 1997

European Journal of Immunogenetics

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The flow cytometric detection of alloantibodies in screening for renal transplantation

et al. 1995

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Flow cytometric screening of sera using pooled chronic lymphocytic leukaemia (CLL) cells has previously been reported as a quick method for detecting HLA antibodies of the IgG class. In this study we investigated the sensitivity of this method in the detection of IgG and IgM alloantibodies, and its performance in serum screening when compared to conventional microlymphocytotoxic screening. Results indicate that flow cytometric screening is more sensitive in the measurement of IgG alloantibodies by up to five doubling dilutions, whereas the converse is true for IgM. IgM autoantibodies were found not to be detectable by flow cytometry. By testing a large number of sera by both methods in parallel, we have found that a significant proportion of sera exhibiting no activity of IgM activity alone on cytotoxic screening contain IgG antibodies detectable with a pool of CLL cells on the flow cytometer.

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A human hybridoma monoclonal antibody (TrJ11) recognizing a new HLA-DR epitope shared by DR4, DR8, DR11, and DRB1∗1303

Bratlie²,

Hannestad

1995

Human Immunology

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The production of a human monoclonal antibody defining a split of HLA‐DRw13 (DRw13b)

Cited by 6 publications

References 8 publications

The Specificity of Anti‐hla Class Ii Monoclonal Antibodies in Cattle

The Specificity of Anti‐hla Class Ii Monoclonal Antibodies in Cattle

The flow cytometric detection of alloantibodies in screening for renal transplantation

A human hybridoma monoclonal antibody (TrJ11) recognizing a new HLA-DR epitope shared by DR4, DR8, DR11, and DRB1∗1303

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