The Process of Osteoblastic Infection by <i>Staphylococcus Aureus</i>

Wen, Qiangqiang; Gu, Feng; Sui, Zhenjiang; Su, Zilong; Yu, Tiecheng

doi:10.7150/ijms.45960

Cited by 27 publications

(22 citation statements)

References 37 publications

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“…The distribution of cellular integrins in vitro (two-dimensional [2D]) differ from the 3D in vivo situation, and integrins are also subject to constant change in vivo . For example, they are exposed in osteoblasts, particularly after fractures, to allow cell contact and wound closure ( 45 ). Recently, it has been described that Fn also has an intrinsic capacity for self-assembly into amyloid-like structures under certain conditions, and amyloid-like Fn deposits also have been found in the liver of patients ( 46 – 48 ).…”

Section: Discussionmentioning

confidence: 99%

More Is Not Always Better—the Double-Headed Role of Fibronectin in Staphylococcus aureus Host Cell Invasion

Niemann

Nguyen

Eble

et al. 2021

mBio

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Section: Discussionmentioning

confidence: 99%

More Is Not Always Better—the Double-Headed Role of Fibronectin in Staphylococcus aureus Host Cell Invasion

Niemann

Nguyen

Eble

et al. 2021

mBio

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“…The presence of proteins and glycans—such as type I collagen, bone sialoprotein, osteopontin and fibronectin—make the EBM a perfect niche for S. aureus that binds these EBM components through multiple adhesins known as microbial surface components which recognize adhesive matrix molecules (MSCRAMMs) [ 8 , 12 ]. Indeed, the S. aureus attachment to the EBM represents a key step in the onset of osteomyelitis, where type I collagen represents approximately 90–95% of the organic fraction of the EBM directly interacting with this pathogen ( Figure 1 ).…”

Section: Introductionmentioning

confidence: 99%

Staphylococcus aureus Internalization in Osteoblast Cells: Mechanisms, Interactions and Biochemical Processes. What Did We Learn from Experimental Models?

et al. 2021

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Bacterial internalization is a strategy that non-intracellular microorganisms use to escape the host immune system and survive inside the human body. Among bacterial species, Staphylococcus aureus showed the ability to interact with and infect osteoblasts, causing osteomyelitis as well as bone and joint infection, while also becoming increasingly resistant to antibiotic therapy and a reservoir of bacteria that can make the infection difficult to cure. Despite being a serious issue in orthopedic surgery, little is known about the mechanisms that allow bacteria to enter and survive inside the osteoblasts, due to the lack of consistent experimental models. In this review, we describe the current knowledge about S. aureus internalization mechanisms and various aspects of the interaction between bacteria and osteoblasts (e.g., best experimental conditions, bacteria-induced damages and immune system response), focusing on studies performed using the MG-63 osteoblastic cell line, the best traditional (2D) model for the study of this phenomenon to date. At the same time, as it has been widely demonstrated that 2D culture systems are not completely indicative of the dynamic environment in vivo, and more recent 3D models—representative of bone infection—have also been investigated.

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“…Therefore, there is an urgent demand to develop novel therapies to treat intracellular S. aureus infections. The process of S. aureus internalization can essentially be divided into three phases ( Figure 1 ): (i) S. aureus binds to the extracellular matrix (ECM), (ii) fibronectin (Fn) receptors mediate S. aureus internalization into cells, and (iii) intracellular S. aureus and persistence into cells ( Wen et al, 2020 ). We will use osteoblasts as an example to illustrate the process of S. aureus internalization.…”

Section: Introductionmentioning

confidence: 99%

Non-antibiotic strategies for prevention and treatment of internalized Staphylococcus aureus

Wen

et al. 2022

Front. Microbiol.

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Staphylococcus aureus (S. aureus) infections are often difficult to cure completely. One of the main reasons for this difficulty is that S. aureus can be internalized into cells after infecting tissue. Because conventional antibiotics and immune cells have difficulty entering cells, the bacteria can survive long enough to cause recurrent infections, which poses a serious burden in healthcare settings because repeated infections drastically increase treatment costs. Therefore, preventing and treating S. aureus internalization is becoming a research hotspot. S. aureus internalization can essentially be divided into three phases: (1) S. aureus binds to the extracellular matrix (ECM), (2) fibronectin (Fn) receptors mediate S. aureus internalization into cells, and (3) intracellular S. aureus and persistence into cells. Different phases require different treatments. Many studies have reported on different treatments at different phases of bacterial infection. In the first and second phases, the latest research results show that the cell wall-anchored protein vaccine and some microbial agents can inhibit the adhesion of S. aureus to host cells. In the third phase, nanoparticles, photochemical internalization (PCI), cell-penetrating peptides (CPPs), antimicrobial peptides (AMPs), and bacteriophage therapy can effectively eliminate bacteria from cells. In this paper, the recent progress in the infection process and the prevention and treatment of S. aureus internalization is summarized by reviewing a large number of studies.

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The Process of Osteoblastic Infection by Staphylococcus Aureus

Cited by 27 publications

References 37 publications

More Is Not Always Better—the Double-Headed Role of Fibronectin in Staphylococcus aureus Host Cell Invasion

More Is Not Always Better—the Double-Headed Role of Fibronectin in Staphylococcus aureus Host Cell Invasion

Staphylococcus aureus Internalization in Osteoblast Cells: Mechanisms, Interactions and Biochemical Processes. What Did We Learn from Experimental Models?

Non-antibiotic strategies for prevention and treatment of internalized Staphylococcus aureus

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