More Is Not Always Better—the Double-Headed Role of Fibronectin in Staphylococcus aureus Host Cell Invasion

Niemann, Silke; Nguyen, Minh Thu; Eble, Johannes A.; Chasan, Achmet Imam; Mrakovcic, Maria; Böttcher, Ralph T.; Preissner, Klaus T.; Roßlenbroich, Steffen; Peters, Georg; Herrmann, Mathias

doi:10.1128/mbio.01062-21

Cited by 17 publications

(22 citation statements)

References 58 publications

(65 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This bridge facilitates the recognition between pathogen and host cell, leading to subsequent cell integration [ 162 , 163 , 164 , 165 ]. Although the osteoblasts evidenced high expression of α5β1-integrin and fibronectin, and the bacteria disclosed a high affinity to adhere to osteoblasts, Niemann et al demonstrated, through internalization tests and immunofluorescence microscopy, that S. aureus was less swallowed in osteoblasts compared to epithelial cells [ 166 ].…”

Section: Pathophysiologymentioning

confidence: 99%

“…The investigators reported a significant increase in osteoblast uptake of S. aureus , resulting in inhibition of fibril formation, brief reduction in RNA-mediated fibronectin expression, and disruption of the fibronectin–fibril network. From the work of Nieman et al it emerges that the fibronectin–fibril network appears to strongly reduce the absorption of S. aureus in a given host cell, indicating that the supramolecular structure of the fibronectin can direct the different ability of particular host cells to internalize the pathogen [ 166 ].…”

Section: Pathophysiologymentioning

confidence: 99%

“…These results can explain the great variability expressed in the efficacy of S. aureus absorption, considering different types of host cells. Again, differences were found in vivo between the courses of bacterial infections and the localization of bacteria in different clinical settings [ 166 ].…”

Section: Pathophysiologymentioning

confidence: 99%

See 2 more Smart Citations

Infective Endocarditis in High-Income Countries

et al. 2022

View full text Add to dashboard Cite

Infective endocarditis remains an illness that carries a significant burden to healthcare resources. In recent times, there has been a shift from Streptococcus sp. to Staphylococcus sp. as the primary organism of interest. This has significant consequences, given the virulence of Staphylococcus and its propensity to form a biofilm, rendering non-surgical therapy ineffective. In addition, antibiotic resistance has affected treatment of this organism. The cohorts at most risk for Staphylococcal endocarditis are elderly patients with multiple comorbidities. The innovation of transcatheter technologies alongside other cardiac interventions such as implantable devices has contributed to the increased risk attributable to this cohort. We examined the pathophysiology of infective endocarditis carefully. Inter alia, the determinants of Staphylococcus aureus virulence, interaction with host immunity, as well as the discovery and emergence of a potential vaccine, were investigated. Furthermore, the potential role of prophylactic antibiotics during dental procedures was also evaluated. As rates of transcatheter device implantation increase, endocarditis is expected to increase, especially in this high-risk group. A high level of suspicion is needed alongside early initiation of therapy and referral to the heart team to improve outcomes.

show abstract

Section: Pathophysiologymentioning

confidence: 99%

Section: Pathophysiologymentioning

confidence: 99%

See 1 more Smart Citation

Infective Endocarditis in High-Income Countries

et al. 2022

View full text Add to dashboard Cite

show abstract

“…No differences were evident between osteoblasts and osteocytes directly after host cell invasion. A possible mechanism for S. aureus invasion into osteoblasts and osteocytes might be the recognition of α5β1 integrins on host cells through fibronectin binding proteins (FnBPA and FnBPB;Niemann et al, 2021). However, further experiments should be performed to investigate this pathway in the present model.…”

Section: Discussionmentioning

confidence: 98%

Osteocytes Serve as a Reservoir for Intracellular Persisting Staphylococcus aureus Due to the Lack of Defense Mechanisms

et al. 2022

View full text Add to dashboard Cite

Chronic staphylococcal osteomyelitis can persist for long time periods causing bone destruction. The ability of Staphylococcus aureus to develop chronic infections is linked to its capacity to invade and replicate within osteoblasts and osteocytes and to switch to a dormant phenotype called small colony variants. Recently, osteocytes were described as a main reservoir for this pathogen in bone tissue. However, the mechanisms involved in the persistence of S. aureus within these cells are still unknown. Here, we investigated the interaction between S. aureus and osteoblasts or osteocytes during infection. While osteoblasts are able to induce a strong antimicrobial response and eliminate intracellular S. aureus, osteocytes trigger signals to recruit immune cells and enhance inflammation but fail an efficient antimicrobial activity to clear the bacterial infection. Moreover, we found that extracellular signals from osteocytes enhance intracellular bacterial clearance by osteoblasts. Even though both cell types express Toll-like receptor (TLR) 2, the main TLR responsible for S. aureus detection, only osteoblasts were able to increase TLR2 expression after infection. Additionally, proteomic analysis indicates that reduced intracellular bacterial killing activity in osteocytes is related to low antimicrobial peptide expression. Nevertheless, high levels of lipid mediators and cytokines were secreted by osteocytes, suggesting that they can contribute to inflammation. Taken together, our results demonstrate that osteocytes contribute to severe inflammation observed in osteomyelitis and represent the main niche for S. aureus persistence due to their poor capacity for intracellular antimicrobial response.

show abstract

“…Between these, evidence proved that IsdB-expressing bacteria bound to both soluble and immobilized vWF. [167] More recently Alfeo et al discovered that the iron-regulated surface determinant B (IsdB) protein, besides being involved in iron transport and vitronectin binding, interacts with von Willebrand Factor (vWF). [168] The researchers found that the bond established between IsdB and the recombinant vWF was stopped by heparin and was reduced due to high ionic strength.…”

Section: Immuno Response and Vaccinementioning

confidence: 99%

Pathophysiology of Infective Endocarditis in High-Income Countries

Nappi¹,

Martuscelli²,

Bellomo³

et al. 2022

Preprint

View full text Add to dashboard Cite

Infective endocarditis remains an illness that carries a significant burden to healthcare resources. In recent times, there has been a shift from Streptococcus sp to Staphylococcus sp as the primary organism of interest. This has significant consequences given the virulence of Staphylococcus and its propensity to form a biofilm, rendering non-surgical therapy ineffective. In addition, antibiotic resistance has affected treatment of this organism. The cohorts at most risk for Staphylococcal endocarditis are the elderly patients with multiple comorbidities. The innovation of transcatheter technologies alongside other cardiac interventions such as implantable devices have contributed to the increased risk attributable to this cohort. We examine the role of the heart team for diagnosis and treatment of this condition. In addition, we examine the determinants of virulence of Staphylococcus aureus, the interaction with hosts immunity and the discovery and emergence of a potential vaccine. We also examine the potential role of prophylactic antibiotics during dental procedures. With increasing rates of transcatheter device implantations, there is a projected increment of endocarditis especially in this high-risk group. A high index of suspicion is needed alongside early initiation of therapy and referral to the heart time to improve outcomes.

show abstract

More Is Not Always Better—the Double-Headed Role of Fibronectin in Staphylococcus aureus Host Cell Invasion

Cited by 17 publications

References 58 publications

Infective Endocarditis in High-Income Countries

Infective Endocarditis in High-Income Countries

Osteocytes Serve as a Reservoir for Intracellular Persisting Staphylococcus aureus Due to the Lack of Defense Mechanisms

Pathophysiology of Infective Endocarditis in High-Income Countries

Contact Info

Product

Resources

About