The Probiotic Strain Bifidobacterium animalis ssp. lactis HY8002 Potentially Improves the Mucosal Integrity of an Altered Intestinal Microbial Environment

Abstract

Intestinal microbiota mediate the development and regulation of the intestinal immune system either directly or indirectly. Particularly, Bifidobacterium spp. play an important role in regulating the intestinal immunity and intestinal barrier. We demonstrated that Bifidobacterium animalis ssp. lactis HY8002, selected from eight Bifidobacterium strains by in vitro experimentation, had exceptional resistance to digestive tract conditions and high adhesion to intestinal epithelial cells and a positive effect on immunoglobulin A (IgA) secretion by Peyer’s patch cells. Moreover, HY8002 restored the expression of tight junction-related genes, initially reduced by lipopolysaccharide treatment, to normal levels in human intestinal epithelial cells. Notably, HY8002 restored kanamycin-induced reduction in Peyer’s patch cell numbers, serum and fecal IgA levels, and zonula occludens 1 and Toll-like receptor 2 levels in the mouse small intestine. In addition, HY8002 restores microbiome composition disturbed by kanamycin, and these microbiome changes have been found to correlate with TLR2 levels in the small intestine. Moreover, the ability of HY8002 to enhance IgA in Peyer’s patch cells and ZO-1 levels in intestinal epithelial cells was significantly inhibited by a TLR2 blocking antibody, which suggests that the HY8002 improve intestinal barrier function via TLR2. Finally, whole-genome sequencing of HY8002 revealed that it did not possess any known virulence factors. Therefore, HY8002 is a promising, functional probiotic supplement to improve intestinal barrier function by improving intestinal immunity and microbiota balance.

Lactobacillus paracaseiinfluences the gut-microbiota-targeted metabolic modulation of the immune status of diarrheal mice

“…Increasing evidence has suggested that the gut microbiota is involved in animal health and disease by regulating nutrient metabolism and immune homeostasis 73 and intestinal microflora dysbiosis plays an important role in the progression of gut diseases. 74 E. coli-induced diarrhea is related to gut microflora and immune response, 75 and probiotics interfere with intestinal flora to enhance the intestinal mucosal barrier and immunity, 76,77 this may provide a possible relationship between probiotics and immune enhancement. This study confirmed the protective effect of L. paracasei against E. coli O8-induced diarrhea, including reduced expression of D-LA and zounlin, 78,79 which indicate intestinal permeability.…”

Probiotic properties and antioxidant activities of Lactiplantibacillus plantarum HY7720 and its fermentation characteristics in plant-based materials

“…The adhesion ability to human intestinal epithelial cells was assessed as previously reported [24]. Brie y, human colonic carcinoma Caco-2 cells were obtained from the ATCC and maintained in MEM supplemented with 10% FBS and 1% antibiotic-antimycotic at 37°C under 5% CO 2 .…”

Lactobacillus helveticus HY7801 ameliorates bacterial vaginosis by inhibiting biofilm formation and epithelial cell adhesion of Gardnerella vaginalis

“…The digestive stability of LAB strains was assessed by measuring their viability under simulated human gastrointestinal conditions. This experiment was performed with slight modifications to a previously described method (Kim et al, 2022). First, each strain suspended in saline (1.0 × 10 9 CFU/ mL) was poured into a 50-mL conical tube, and 26 μL of 0.3 M CaCl 2 solution (Sigma-Aldrich) and 4 mL of 6.55 mg/ mL α-amylase solution (Sigma-Aldrich) were added.…”