The pro-apoptotic and anti-invasive effects of hypericin-mediated photodynamic therapy are enhanced by hyperforin or aristoforin in HT-29 colon adenocarcinoma cells

Šemeláková, Martina; Mikeš, Jaromír; Jendželovský, Rastislav; Fedoročko, Peter

doi:10.1016/j.jphotobiol.2012.09.003

Cited by 28 publications

(9 citation statements)

References 30 publications

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“…The high degree of cytotoxicity observed in 1 μ g/ml irradiated HY-treated HepG2 cells could be owing to alternations in the apoptotic mechanisms that reportedly render the cells more sensitive to HY-PDT. 18 A recent study reviewed that HY has little noticeable biological activity in the absence of light and enhances the degree of undesirable side-effects on non-target tissues. 19 …”

Section: Discussionmentioning

confidence: 99%

Hypericin-photodynamic therapy leads to interleukin-6 secretion by HepG2 cells and their apoptosis via recruitment of BH3 interacting-domain death agonist and caspases

et al. 2013

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Photodynamic therapy (PDT) has emerged as a capable therapeutic modality for the treatment of cancer. PDT is a targeted cancer therapy that reportedly leads to tumor cell apoptosis and/or necrosis by facilitating the secretion of certain pro-inflammatory cytokines and expression of multiple apoptotic mediators in the tumor microenvironment. In addition, PDT also triggers oxidative stress that directs tumor cell killing and activation of inflammatory responses. However, the cellular and molecular mechanisms underlying the role of PDT in facilitating tumor cell apoptosis remain ambiguous. Here, we investigated the ability of PDT in association with hypericin (HY) to induce tumor cell apoptosis by facilitating the induction of reactive oxygen species (ROS) and secretion of Th1/Th2/Th17 cytokines in human hepatocellular liver carcinoma cell line (HepG2) cells. To discover if any apoptotic mediators were implicated in the enhancement of cell death of HY-PDT-treated tumor cells, selected gene profiling in response to HY-PDT treatment was implemented. Experimental results showed that interleukin (IL)-6 was significantly increased in all HY-PDT-treated cells, especially in 1 μg/ml HY-PDT, resulting in cell death. In addition, quantitative real-time PCR analysis revealed that the expression of apoptotic genes, such as BH3-interacting-domain death agonist (BID), cytochrome complex (CYT-C) and caspases (CASP3, 6, 7, 8 and 9) was remarkably higher in HY-PDT-treated HepG2 cells than the untreated HepG2 cells, entailing that tumor destruction of immune-mediated cell death occurs only in PDT-treated tumor cells. Hence, we showed that HY-PDT treatment induces apoptosis in HepG2 cells by facilitating cytotoxic ROS, and potentially recruits IL-6 and apoptosis mediators, providing additional hints for the existence of alternative mechanisms of anti-tumor immunity in hepatocellular carcinoma, which contribute to long-term suppression of tumor growth following PDT.

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Section: Discussionmentioning

confidence: 99%

Hypericin-photodynamic therapy leads to interleukin-6 secretion by HepG2 cells and their apoptosis via recruitment of BH3 interacting-domain death agonist and caspases

et al. 2013

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“…Cytotoxicity of photoactivated hypericin is higher than that of 5aminolevulinic acid, as its LD 50 in medullablastoma cells was 8-13 times lower, with 0.47 J/cm² after 2 h exposure to 2.5 μM hypericin [65]. The effect of photoactivated hypericin treatment in HT-29 colon adenoma cells was boosted by the addition of 5 μM hyperforin or adhyperforin, resulting in apoptosis induction, inhibition of cell cycle progression, and expression of MMP-/-9 together with cell adhesivity, thereby affecting the clonogenic potential of the cells [66]. Currently, hypericin-PDT is intensively studied as a diagnostic and therapeutic tool for dermal and for internal cancer species.…”

Section: Anticancer Effectsmentioning

confidence: 99%

Topical Application of St. Johnʼs Wort (Hypericum perforatum)

Seelinger²,

2013

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St. John's wort (Hypericum perforatum) has been intensively investigated for its antidepressive activity, but dermatological applications also have a long tradition. Topical St. John's wort preparations such as oils or tinctures are used for the treatment of minor wounds and burns, sunburns, abrasions, bruises, contusions, ulcers, myalgia, and many others. Pharmacological research supports the use in these fields. Of the constituents, naphthodianthrones (e.g., hypericin) and phloroglucinols (e.g., hyperforin) have interesting pharmacological profiles, including antioxidant, anti-inflammatory, anticancer, and antimicrobial activities. In addition, hyperforin stimulates growth and differentiation of keratinocytes, and hypericin is a photosensitizer which can be used for selective treatment of nonmelanoma skin cancer. However, clinical research in this field is still scarce. Recently, sporadic trials have been conducted in wound healing, atopic dermatitis, psoriasis, and herpes simplex infections, partly with purified single constituents and modern dermatological formulations. St. John's wort also has a potential for use in medical skin care. Composition and stability of pharmaceutical formulations vary greatly depending on origin of the plant material, production method, lipophilicity of solvents, and storage conditions, and this must be regarded with respect to practical as well as scientific purposes.

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“…Продолжаются исследования цитотоксической активности отдельных компонентов H. perforatum в фотодинамической терапии. Так, в частности, гиперфорин и аристофолин (синтетическое производное гиперфорина) при фотодинамической терапии с использованием гиперицина стимулировали апоптоз клеток линии HT-29 (аденокарцинома толстой кишки) [101] и гибель как непигментированных (A375 и 501mel), так и пигментированных (UCT Mel-1) клеток меланомы [102] 1 . Фотоактивирован-ный гиперицин вызывал апоптоз клеток линий RINm5F (инсулинома) [103], SCC (чешуйчатая карцинома человека) [104], D273 (медуллобластома) [105] и оказался эффективным при анапластическом раке щитовидной железы [106].…”

Section: противоопухолевые и цитотоксические свойстваunclassified

Biological Activity of Hypericum Perforatum L. (Hypericaceae): A Review

Буданцев¹,

Prikhodko

Варганова³

et al. 2021

Farm. farmakol. (Pâtigorsk)

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Федеральное государственное бюджетное учреждение науки Ботанический институт им. В.Л. Комарова Российской академии наук (БИН РАН) 197376, Россия, г. Санкт-Петербург, ул. проф. Попова, 2 2 Федеральное государственное бюджетное образовательное учреждение высшего образования «Санкт-Петербургский государственный химико-фармацевтический университет»

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The pro-apoptotic and anti-invasive effects of hypericin-mediated photodynamic therapy are enhanced by hyperforin or aristoforin in HT-29 colon adenocarcinoma cells

Cited by 28 publications

References 30 publications

Hypericin-photodynamic therapy leads to interleukin-6 secretion by HepG2 cells and their apoptosis via recruitment of BH3 interacting-domain death agonist and caspases

Hypericin-photodynamic therapy leads to interleukin-6 secretion by HepG2 cells and their apoptosis via recruitment of BH3 interacting-domain death agonist and caspases

Topical Application of St. Johnʼs Wort (Hypericum perforatum)

Biological Activity of Hypericum Perforatum L. (Hypericaceae): A Review

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