The pro-angiogenic properties of multi-functional bioactive glass composite scaffolds

Gerhardt, Lutz-Christian; Widdows, Kate; Erol, M.; Burch, Charles W.; Sanz-Herrera, José A.; Ochoa, Ignacio; Stämpfli, Rolf; Roqan, Iman S.; Gabe, Simon; Ansari, Tahera; Boccaccını, Aldo R.

doi:10.1016/j.biomaterials.2011.02.032

Cited by 174 publications

(128 citation statements)

References 62 publications

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“…As a result, POC-BG-30 % had the highest porosity of approximately 86 %. This is consistent with the previous study [33] where 20 % incorporation of bioglass into PDLLA led to an increase in porosity and it was more apparent for the nanocomposites in respect to microcomposites [33]. However, the work by Srinivasan et al showed a decrease in porosity ratio by addition of more nano-bioglass [30].…”

Section: Thermal Analysissupporting

confidence: 79%

Fabrication and characterization of poly(octanediol citrate)/gallium-containing bioglass microcomposite scaffolds

et al. 2014

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Bone can be affected by osteosarcomae requiring surgical excision of the tumor as part of the treatment regime. Complete removal of cancerous cells is difficult and conventionally requires the removal of a margin of safety around the tumor to offer improved patient prognosis. This work considers a novel series of composite scaffolds based on poly(octanediol citrate) (POC) impregnated with galliumbased bioglass microparticles for possible incorporation into bone following tumor removal. The objective of this research was to fabricate and characterize these scaffolds and subsequently report on their mechanical and biological properties. The porous microcomposite scaffolds with various concentrations of bioglass (10, 20, 30 wt%) incorporated were fabricated using a salt leaching technique. The scaffolds exhibited compression modulus in the range of 0.3-7 MPa. The addition of bioglass increased the mechanical properties even though porosity increased. Furthermore, increasing the concentration of bioglass had a significant influence on glass transition temperature from 2.5°C for the pure polymer to around 25°C for 30 % bioglass-containing composite. The ion release study revealed that composites containing 10 % bioglass had the highest ion release ratio after 28 days of soaking in phosphate buffered saline. The interaction of bioglass phase with POC led to the formation of additional ionic crosslinks aside from covalent crosslinks which further resulted in increased stiffness and decreased weight loss. The osteoblast cells were well attached and growth on composites and collagen synthesis increased particularly with the 10 % bioglass concentration.

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Section: Thermal Analysissupporting

confidence: 79%

Fabrication and characterization of poly(octanediol citrate)/gallium-containing bioglass microcomposite scaffolds

et al. 2014

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“…1D). Volume fractions were used as a measure of the functionality of the angiogenic response by the host toward the scaffold, representing the volume of implant occupied by blood vessels (31). The efficiency of the angiogenic response toward DT was confirmed by the chicken egg chorioallantoic membrane (CAM) assay ( Fig.…”

Section: Decellularization Slows Biodegradation Time In Vivo and Prommentioning

confidence: 99%

Immunomodulatory effect of a decellularized skeletal muscle scaffold in a discordant xenotransplantation model

Fishman

Lowdell

Urbani³

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

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Decellularized (acellular) scaffolds, composed of natural extracellular matrix, form the basis of an emerging generation of tissueengineered organ and tissue replacements capable of transforming healthcare. Prime requirements for allogeneic, or xenogeneic, decellularized scaffolds are biocompatibility and absence of rejection. The humoral immune response to decellularized scaffolds has been well documented, but there is a lack of data on the cellmediated immune response toward them in vitro and in vivo. Skeletal muscle scaffolds were decellularized, characterized in vitro, and xenotransplanted. The cellular immune response toward scaffolds was evaluated by immunohistochemistry and quantified stereologically. T-cell proliferation and cytokines, as assessed by flow cytometry using carboxy-fluorescein diacetate succinimidyl ester dye and cytometric bead array, formed an in vitro surrogate marker and correlate of the in vivo host immune response toward the scaffold. Decellularized scaffolds were free of major histocompatibility complex class I and II antigens and were found to exert anti-inflammatory and immunosuppressive effects, as evidenced by delayed biodegradation time in vivo; reduced sensitized T-cell proliferative activity in vitro; reduced IL-2, IFN-γ, and raised IL-10 levels in cell-culture supernatants; polarization of the macrophage response in vivo toward an M2 phenotype; and improved survival of donor-derived xenogeneic cells at 2 and 4 wk in vivo. Decellularized scaffolds polarize host responses away from a classical TH1-proinflammatory profile and appear to down-regulate T-cell xeno responses and TH1 effector function by inducing a state of peripheral T-cell hyporesponsiveness. These results have substantial implications for the future clinical application of tissue-engineered therapies.

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“…[6][7][8][9] More recently, studies have shown that bioactive glasses may also be effective in promoting the vascularization of tissue constructs in different combinations, for example, as sintered scaffolds and composites. [10][11][12][13][14][15][16][17] The use of Bioglass Ò to fabricate scaffolds using a foam replica method was introduced in 2006 followed by studies on the possible angiogenic effect of such scaffolds. 18 Similar bioactive glass scaffolds were investigated in coculture studies using human umbilical vein endothelial cells (HUVEC) and human osteoblasts, 14 and it was shown that the scaffolds were able to support both endothelial and human osteoblast proliferation.…”

mentioning

confidence: 99%

45S5-Bioglass^®-Based 3D-Scaffolds Seeded with Human Adipose Tissue-Derived Stem Cells Induce In Vivo Vascularization in the CAM Angiogenesis Assay

Handel

Hammer

Nooeaid

et al. 2013

Tissue Engineering Part A

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Poor vascularization is the key limitation for long-term acceptance of large three-dimensional (3D) tissue engineering constructs in regenerative medicine. 45S5 Bioglass Ò was investigated given its potential for applications in bone engineering. Since native Bioglass Ò shows insufficient angiogenic properties, we used a collagen coating, to seed human adipose tissue-derived stem cells (hASC) confluently onto 3D 45S5 Bioglass Ò -based scaffolds. To investigate vascularization by semiquantitative analyses, these biofunctionalized scaffolds were then subjected to in vitro human umbilical vein endothelial cells formation assays, and were also investigated in the chorioallantoic membrane (CAM) angiogenesis model, an in vivo angiogenesis assay, which uses the CAM of the hen's egg. In their native, nonbiofunctionalized state, neither Bioglass Ò -based nor biologically inert fibrous polypropylene control scaffolds showed angiogenic properties. However, significant vascularization was induced by hASC-seeded scaffolds (Bioglass Ò and polypropylene) in the CAM angiogenesis assay. Biofunctionalized scaffolds also showed enhanced tube lengths, compared to unmodified scaffolds or constructs seeded with fibroblasts. In case of biologically inert hernia meshes, the quantification of vascular endothelial growth factor secretion as the key angiogenic stimulus strongly correlated to the tube lengths and vessel numbers in all models. This correlation proved the CAM angiogenesis assay to be a suitable semiquantitative tool to characterize angiogenic effects of larger 3D implants. In addition, our results suggest that combinations of suitable scaffold materials, such as 45S5 Bioglass Ò , with hASC could be a promising approach for future tissue engineering applications.

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The pro-angiogenic properties of multi-functional bioactive glass composite scaffolds

Cited by 174 publications

References 62 publications

Fabrication and characterization of poly(octanediol citrate)/gallium-containing bioglass microcomposite scaffolds

Fabrication and characterization of poly(octanediol citrate)/gallium-containing bioglass microcomposite scaffolds

Immunomodulatory effect of a decellularized skeletal muscle scaffold in a discordant xenotransplantation model

45S5-Bioglass^®-Based 3D-Scaffolds Seeded with Human Adipose Tissue-Derived Stem Cells Induce In Vivo Vascularization in the CAM Angiogenesis Assay

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The pro-angiogenic properties of multi-functional bioactive glass composite scaffolds

Cited by 174 publications

References 62 publications

Fabrication and characterization of poly(octanediol citrate)/gallium-containing bioglass microcomposite scaffolds

Fabrication and characterization of poly(octanediol citrate)/gallium-containing bioglass microcomposite scaffolds

Immunomodulatory effect of a decellularized skeletal muscle scaffold in a discordant xenotransplantation model

45S5-Bioglass®-Based 3D-Scaffolds Seeded with Human Adipose Tissue-Derived Stem Cells Induce In Vivo Vascularization in the CAM Angiogenesis Assay

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Product

Resources

About

45S5-Bioglass^®-Based 3D-Scaffolds Seeded with Human Adipose Tissue-Derived Stem Cells Induce In Vivo Vascularization in the CAM Angiogenesis Assay