The primary interaction of poliomyelitis virus with host cells of tissue culture origin

Bachtold, J. G.; Bubel, H. Curt; Gebhardt, L. P.

doi:10.1016/0042-6822(57)90087-9

Cited by 44 publications

(14 citation statements)

References 7 publications

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“…The presence of Mg and Ca ions in the adsorption medium improved the adsorption rate, coinciding the observations with other virus-cell systems [1,4,5].…”

Section: Discussionsupporting

confidence: 69%

“…Measles virus readily adsorbed onto host cells in various media containing electrolytes, but not in glucose solution, a fact indicating that the adsorption of measles virus onto host cells, like other viruses, is dependent on electrolytes [1,5,12]. The presence of Mg and Ca ions in the adsorption medium improved the adsorption rate, coinciding the observations with other virus-cell systems [1,4,5].…”

Section: Discussionsupporting

confidence: 67%

“…Lower temperatures slowed adsorption of measles virus onto host cells but only to a slight extent as previously shown in other virus-cell systems [5,12,20]. However, the number of plaques developing after adsorption at 3 C or 12 C was readily diminished by mere washing before agar overlay.…”

Section: Discussionmentioning

confidence: 65%

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Plaque Formation and Initial Virus‐Cell Interaction of Measles Virus

Arita

Matumoto

1968

Japanese Journal of Microbiology

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The Sugiyama strain of measles virus adapted to FL cells produces plaques heterogeneous in size and morphology on FL and other stable human cell line monolayers making it difficult to assay by the plaque technique. A virus clone producing homogeneous clear plaques was established by repeated cloning of the strain. A practical reliable plaque assay method was established for this cloned line. FL cells were found to be well suited for this purpose, but Vero cells, a stable line of African green monkey kidney cells, were shown to be more sensitive than FL cells. HeLa cells could be used for the assay, but were less sensitive than the Vero or FL cell line. The initial step of measles virus adsorption onto host cells was shown to be reversible, dependent on electrolytes and almost temperature-independent, strongly suggesting adsorption is electrostatic in nature. At 36 C the initial stage of adsorption quickly passed into the next irreversible stage involving a temperature-dependent reaction; virus adsorbed at 36 C soon became resistant to the action of measles antibody, whereas little virus adsorbed at 4 C or 12 C became antibody-resistant. The time required for the adsorbed virus to move into the irreversible, antibody-resistant stage seems very short.

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“…The presence of Mg and Ca ions in the adsorption medium improved the adsorption rate, coinciding the observations with other virus-cell systems [1,4,5].…”

Section: Discussionsupporting

confidence: 69%

Section: Discussionsupporting

confidence: 67%

See 1 more Smart Citation

Plaque Formation and Initial Virus‐Cell Interaction of Measles Virus

Arita

Matumoto

1968

Japanese Journal of Microbiology

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“…This conclusion is supported by the observation that bypassing the receptor binding step by transfection of cells with RNA permits one cycle of replication in many receptor-negative mammalian cell types (3). A complete understanding of poliovirus replication and pathogenesis therefore requires better knowledge of the structure, function, and expression of the viral receptor that plays an important role in cell and tissue tropism.Early studies showed that tissues and cell types that are susceptible to poliovirus infection contain a membraneassociated activity that is capable of specifically binding poliovirus (4)(5)(6). Subsequent studies have shown that the virus binding activity, or receptor, is an integral membrane protein (7,8).…”

mentioning

confidence: 99%

“…Early studies showed that tissues and cell types that are susceptible to poliovirus infection contain a membraneassociated activity that is capable of specifically binding poliovirus (4)(5)(6). Subsequent studies have shown that the virus binding activity, or receptor, is an integral membrane protein (7,8).…”

mentioning

confidence: 99%

Transformation of a human poliovirus receptor gene into mouse cells.

Mendelsohn¹,

Johnson²,

Lionetti³

et al. 1986

Proc. Natl. Acad. Sci. U.S.A.

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The first step in poliovirus replication is binding of virus to a cellular receptor. Mouse L cells, which are resistant to poliovirus infection because they do not bear a poliovirus receptor, were transformed with HeLa cell (human) DNA to poliovirus sensitivity at a frequency of 1 in 50,000 transformants. Monoclonal antibody directed against the HeLa cell poliovirus receptor site was used in rosette assays to identify poliovirus-sensitive L-cell transformants in a background of L-cell tk' transformants. A cloned cell line, CM-1, was isolated that displayed a surface component recognized by the antipoliovirus receptor antibody. CM-1 cells were susceptible to infection with all three poliovirus serotypes, and infection could be blocked by the antireceptor antibody. Poliovirus formed plaques in CM-1 and HeLa cells with equal efficiency. CM-1 and HeLa cells produced infectious poliovirus at a similar rate, although yield of virus in CM-1 cells was about 33% less than the yield in HeLa cells. These results suggest that DNA encoding the HeLa cell poliovirus receptor has been introduced into mouse cells, resulting in the expression of the receptor and susceptibility to poliovirus infection.Poliovirus is an icosahedral RNA-containing virus with a host range that is limited to primates and primate cell cultures. In the infected host, viral replication occurs predominantly in the intestinal mucosa, in certain lymphoid tissue, and in the central nervous system (1). A large body of evidence indicates that a cellular receptor is the major determining factor in cell and tissue susceptibility to poliovirus infection (reviewed in ref. 2). This conclusion is supported by the observation that bypassing the receptor binding step by transfection of cells with RNA permits one cycle of replication in many receptor-negative mammalian cell types (3). A complete understanding of poliovirus replication and pathogenesis therefore requires better knowledge of the structure, function, and expression of the viral receptor that plays an important role in cell and tissue tropism.Early studies showed that tissues and cell types that are susceptible to poliovirus infection contain a membraneassociated activity that is capable of specifically binding poliovirus (4)(5)(6). Subsequent studies have shown that the virus binding activity, or receptor, is an integral membrane protein (7,8). There are -3000 receptor sites on the HeLa cell membrane, but it is not known how many receptors comprise a binding site (9). The three poliovirus serotypes compete for a binding site that is distinct from that of other enteroviruses (10-13). Recently, a receptor protein from coxsackievirus B3, an enterovirus related to poliovirus, was purified from HeLa cells (14). Attempts to isolate and characterize the poliovirus receptor have not been successful, probably because there are so few receptors per cell and because so far it has not been possible to measure virus binding activity in the presence of detergents (7).To circumvent the difficulties associated wit...

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Poliovirus and its cellular receptor: a molecular genetic dissection of a virus/receptor affinity interaction

et al. 1998

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The ability of a virus to attach to a susceptible host cell is of utmost importance for the initiation of viral life cycle. Cell surface proteins called viral receptors mediate the initial steps of virus attachment and uptake. Poliovirus (PV) is one of the most studied animal viruses and its interaction with its cellular receptor, the human poliovirus receptor (hPVR) has been well characterized. This review will present our current understanding of the PV/hPVR interaction at the genetic and biochemical level. In addition, we will also discuss the implications of the PV/hPVR interaction on PV tissue tropism and the evolution of the three PV serotypes.

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The primary interaction of poliomyelitis virus with host cells of tissue culture origin

Cited by 44 publications

References 7 publications

Plaque Formation and Initial Virus‐Cell Interaction of Measles Virus

Plaque Formation and Initial Virus‐Cell Interaction of Measles Virus

Transformation of a human poliovirus receptor gene into mouse cells.

Poliovirus and its cellular receptor: a molecular genetic dissection of a virus/receptor affinity interaction

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