The preventive effect of grape seed extract on artificial enamel caries progression in a microbial biofilm-induced caries model

Zhao, Wei; Xie, Qian; Bedran-Russo, Anakarina B.; Pan, Shuang; Ling, Junqi; Wu, Christine D.

doi:10.1016/j.jdent.2014.05.006

Cited by 47 publications

(64 citation statements)

References 67 publications

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“…However, the discrepancy in outcomes raises concerns as to their limitation and thus careful data interpretation is warranted. The use of rhodamine infiltration for quantification of surface porosities has been validated for caries studies of enamel [Fontana et al, 1996;Gonzalez-Cabezas et al, 1998;Zhao et al, 2014] and studied in dentin [Xie et al, 2008]. This method is limited by a 2D surface analysis at relatively low resolution.…”

Section: Discussionmentioning

confidence: 99%

“…Specimens (n = 15) were embedded in epoxy resin and polished on a water-cooled polishing unit (EcoMet 3000; Buehler) with abrasive papers (400, 600, 800 and 1,200 grit). Afterwards, the specimens were stained overnight with a freshly prepared 0.1 mM rhodamine B solution (Aldrich Chemical Co., Milwaukee, WI, USA) [Zhao et al, 2014], and rinsed for 1 min with deionized water. The fluorescence intensity of infiltrated rhodamine B was analyzed under fluorescence microscopy (DMI6000 B, Leica), using red emission and DIC (differential interference contrast) channels.…”

Section: Fluorescence Microscopymentioning

confidence: 99%

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Remineralization Potential of Mints Containing Bioactive Agents in Artificially Induced Root Caries

Zamperini

Bedran-Russo²

2018

Caries Res

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This study investigated the remineralization effect of experimental mint formulations containing bioactive agents (xylitol; green tea extract, GT; and amorphous calcium phosphate, ACP) in the progression of artificially induced root caries. Root caries lesions were induced by demineralization solution (pH 4.6; 96 h; 37°C). The lesions were treated with mint A, mint B, mint C, xylitol, GT, ACP, or remineralization solution (RS; negative control). Specimens were pH-cycled through treatments (5×/day; 3 min) and 6 cycles of acidic (pH 5.0; 30 min) and neutral (pH 7.0; 10 min) buffers for 8 days. Bacterial collagenase (Clostridium histolyticum) was used overnight to simulate proteolytic challenge. Caries depth and porosity as well as mineral density were estimated using fluorescence microscopy (n = 15) and microcomputed tomography (n = 6). Analysis of variance (ANOVA, α = 0.05) showed no statistically significant difference in caries depth among all groups (p = 0.172). The highest fluorescence intensity decrease was observed for GT followed by mint C, with no significant difference between them (p = 0.868). There were significant differences among GT and mints A, B, and C when compared to RS (p < 0.001). No statistically significant differences in fluorescence intensity were observed among ACP, xylitol, and RS (p > 0.05). The mineral density of the lesions in GT, mints A, B, and C, and ACP was statistically similar (p > 0.05) and significantly higher than that in RS (p < 0.05). No significant difference was observed between xylitol and RS (p = 0.728). The experimental mints showed remineralization action on artificial root caries, and GT was found to be the main active ingredient in the investigated formulations.

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Section: Discussionmentioning

confidence: 99%

Section: Fluorescence Microscopymentioning

confidence: 99%

Remineralization Potential of Mints Containing Bioactive Agents in Artificially Induced Root Caries

Zamperini

Bedran-Russo²

2018

Caries Res

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“…Specific to cariogenic bacteria, PACs inhibit surface-adsorbed glucosyltransferases and acid production by S. mutans , 34 as well as decrease the growth of S. mutans and biofilm formation. 35 Catechins such as epigallocatechin gallate suppress gtf genes associated with S. mutans biofilm formation. 36 The antimicrobial activity of PACs against cariogenic bacteria likely contributes to the inhibition of dentin demineralization by e-GSE, as the dentin tissue might function as a reservoir for PACs bound to the collagen backbone.…”

Section: Discussionmentioning

confidence: 99%

Effect of Bioactive Primers on Bacterial-Induced Secondary Caries at the Tooth-Resin Interface

Kim

Leme-Kraus

Phansalkar

et al. 2017

Operative Dentistry

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Summary Secondary caries at the tooth-resin interface is the primary reason for replacement of resin composite restorations. The tooth-resin interface is formed by the interlocking of resin material with hydroxyapatite crystals in enamel and collagen mesh structure in dentin. Efforts to strengthen the tooth-resin interface have identified chemical agents with dentin collagen cross-linking potential and antimicrobial activities. The purpose of the present study was to assess protective effects of bioactive primer against secondary caries development around enamel and dentin margins of class V restorations, using an in vitro bacterial caries model. Class V composite restorations were prepared on 60 bovine teeth (n=15) with pretreatment of the cavity walls with control buffer solution, an enriched fraction of grape seed extract (e-GSE), 1-ethyl-3-(3-dimethyl aminopropyl)-carbodiimide/N-hydroxysuccinimide, or chlorhexidine digluconate. After incubating specimens in a bacterial model with Streptococcus mutans for four days, dentin and enamel were assessed by fluorescence microscopy. Results revealed that only the naturally occurring product, e-GSE, significantly inhibited the development of secondary caries immediately adjacent to the dentin-resin interface, as indicated by the caries inhibition zone. No inhibitory effects were observed in enamel margins. The results suggest that the incorporation of e-GSE into components of the adhesive system may inhibit secondary caries and potentially contribute to the protection of highly vulnerable dentin-resin margins.

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“…These models differ among them mainly regarding the source of bacteria used to form the biofilm and regarding the dynamic of carbohydrate/fluoride exposures during carious lesions development. Whereas monospecies biofilm models based only on cultivation of Streptococcus mutans [16, 17] do not mimic the metabolic interactions that occur among the diverse microbiota of a clinical dental biofilm, the outcomes obtained by high-complexity microbial models based on microcosm cultivation are directly dependent on inoculum source [18, 19]. On the other hand, multispecies biofilms models based on the cultivation of specific microorganisms might create a more controlled and less variable condition for carious lesions development.…”

Section: Introductionmentioning

confidence: 99%

Enamel Carious Lesion Development in Response to Sucrose and Fluoride Concentrations and to Time of Biofilm Formation: An Artificial-Mouth Study

Arthur

Kohara

Waeiss

et al. 2014

Journal of Oral Diseases

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The aim of this study was to evaluate both sucrose and fluoride concentrations and time of biofilm formation on enamel carious lesions induced by an in vitro artificial-mouth caries model. For Study 1, biofilms formed by streptococci and lactobacilli were grown on the surface of human enamel slabs and exposed to artificial saliva containing 0.50 or 0.75 ppmF (22.5 h/d) and broth containing 3 or 5% sucrose (30 min; 3x/d) over 5 d. In Study 2, biofilms were grown in the presence of 0.75 ppmF and 3% sucrose over 3 and 9 days. Counts of viable cells on biofilms, lesion depth (LD), and the integrated mineral loss (IML) on enamel specimens were assessed at the end of the tested conditions. Counts of total viable cells and L. casei were affected by sucrose and fluoride concentrations as well as by time of biofilm formation. Enamel carious lesions were shallower and IML was lower in the presence of 0.75 ppmF than in the presence of 0.50 ppmF (P < 0.005). No significant effect of sucrose concentrations was found with respect to LD and IML (P > 0.25). Additionally, deeper lesions and higher IML were found after 9 d of biofilm formation (P < 0.005). Distinct sucrose concentrations did not affect enamel carious lesion development. The severity of enamel demineralization was reduced by the presence of the higher fluoride concentration. Additionally, an increase in the time of biofilm formation produced greater demineralization. Our results also suggest that the present model is suitable for studying aspects related to caries lesion development.

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The preventive effect of grape seed extract on artificial enamel caries progression in a microbial biofilm-induced caries model

Cited by 47 publications

References 67 publications

Remineralization Potential of Mints Containing Bioactive Agents in Artificially Induced Root Caries

Remineralization Potential of Mints Containing Bioactive Agents in Artificially Induced Root Caries

Effect of Bioactive Primers on Bacterial-Induced Secondary Caries at the Tooth-Resin Interface

Enamel Carious Lesion Development in Response to Sucrose and Fluoride Concentrations and to Time of Biofilm Formation: An Artificial-Mouth Study

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