The Preventive Effect of Ginseng with Du-Zhong Leaf on Protein Metabolism in Aging.

Metori, Koichi; Furutsu, Mio; Takahashi, S.

doi:10.1248/bpb.20.237

Cited by 34 publications

(18 citation statements)

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“…Additionally, supplementation with ginseng and Eucommia bark prevented muscle protein degradation in rats caused by ageing [5]. Coptis rhizome, another component of KST, increases protein synthesis in smooth muscle cells at the phase of accelerating proteasome-mediated degradation [6].…”

Section: Introductionmentioning

confidence: 99%

Herbal supplement Kamishimotsuto augments resistance exercise-induced mTORC1 signaling in rat skeletal muscle

et al. 2016

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Section: Introductionmentioning

confidence: 99%

Herbal supplement Kamishimotsuto augments resistance exercise-induced mTORC1 signaling in rat skeletal muscle

et al. 2016

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“…The bark and leaf of EuO have been used in traditional Chinese medicine to treat lower back pain, knee pain, and the liver, kidney, and spleen disorders. EuO extracts have antihypertensive, antioxidative, and antigastric ulcer effects (Metori et al, 1997;Deyama et al, 2001;Yang et al, 2003). Water extracts of EuO leaves have been reported to have potent antimutagenic effects (Nakamura et al, 1997;Lee et al, 2005;Luo et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

“…Water extracts of EuO leaves have been reported to have potent antimutagenic effects (Nakamura et al, 1997;Lee et al, 2005;Luo et al, 2009). EuO extracts have also been reported to enhance collagen synthesis (Metori et al, 1997;Li et al, 2000a;2000b;Deyama et al, 2001). It is generally assumed that natural substances in EuO give rise to these beneficial effects.…”

Section: Introductionmentioning

confidence: 99%

Eucommia ulmoides Oliv. antagonizes H2O2-induced rat osteoblastic MC3T3-E1 apoptosis by inhibiting expressions of caspases 3, 6, 7, and 9

Lin

Fan

Mehl

et al. 2011

J. Zhejiang Univ. Sci. B

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Eucommia ulmoides Oliv. (EuO), also known as Duzhong, native to China, has been reported to have antioxidative function, but its cellular mechanism is not fully examined yet. We investigated inhibitory effects of EuO leaf ethanol extracts on H 2 O 2 -induced apoptosis in rat osteoblastic MC3T3-E1 cells and underlying mechanisms. Locally-grown Duzhong leaves were extracted with ethanol. MC3T3-E1 cells were treated with EuO (6.25, 12.5, 25, 50, and 100 µg/ml) for 24 h, and then H 2 O 2 (800 µmol/L) for an additional 24 h. Cell survival rate, percentage of apoptosis, and expressions of caspases 3, 6, 7, and 9 were examined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, microscopic analysis, Western blotting, and reverse transcription polymerase chain reaction (RT-PCR). The final EuO leaf ethanol extract powder was detected to contain caffeotannic acid at 58 mg/g and geniposide at 3.45 mg/g by high performance liquid chromatography (HPLC). EuO remarkably restrained cell oxidative damage and increased cell survival rate in a dose-dependent manner: 0 µg/ml, 0.21; 6.25 µg/ml, 0. 28; 12.5 µg/ml, 0.31; 25 µg/ml, 0.48; 50 µg/ml, 0.54; and 100 µg/ml, 0.66 (P<0.05), with the half-effective concentration being around 25 µg/ml. MTT results were confirmed by microscopic analysis. Western blotting and RT-PCR analyses showed that the expressions of caspases 3, 6, 7, and 9 were significantly decreased in the EuO-treated cells compared with the control (EuO-and H 2 O 2 -free) (P<0.05), with the half-effective concentration of EuO ranging from 12.5 to 25 µg/ml. We conclude that the ethanol-extracted EuO leaf extracts promoted the growth of MC3T3-E1 cells, and suppressed the H 2 O 2 -induced apoptosis in a rat MC3T3-E1 osteogenic cell model, likely due to the inhibition of caspases' activities. The results indicate that EuO is a potent antioxidant, which may contribute to its many cellular protective functions, including the promotion of bone growth.

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“…4 A large number of studies about the pharmacological properties of ginsenosides have been reported. [5][6][7][8] The main pharmacological properties of ginsenosides are that they inhibit the central nervous system, 9,10 relieve pain 11 and inhibit cancer-cell reproduction, 12 and they also improve diabetes 13,14 and hypertension. 15 The pharmacologic effects of Panax ginseng are suspected not to result from one kind of ginsenoside, but from both the combination and amount of ginsenosides that it contains.…”

Section: Introductionmentioning

confidence: 99%

Two-dimensional Mapping Using Different Chromatographic Separations Coupled with Mass Spectrometry for the Analysis of Ginsenosides in Panax Ginseng Root and Callus

et al. 2013

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The Preventive Effect of Ginseng with Du-Zhong Leaf on Protein Metabolism in Aging.

Cited by 34 publications

References 0 publications

Herbal supplement Kamishimotsuto augments resistance exercise-induced mTORC1 signaling in rat skeletal muscle

Herbal supplement Kamishimotsuto augments resistance exercise-induced mTORC1 signaling in rat skeletal muscle

Eucommia ulmoides Oliv. antagonizes H2O2-induced rat osteoblastic MC3T3-E1 apoptosis by inhibiting expressions of caspases 3, 6, 7, and 9

Two-dimensional Mapping Using Different Chromatographic Separations Coupled with Mass Spectrometry for the Analysis of Ginsenosides in Panax Ginseng Root and Callus

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