Two-dimensional Mapping Using Different Chromatographic Separations Coupled with Mass Spectrometry for the Analysis of Ginsenosides in Panax Ginseng Root and Callus

Osaka, Issey; Hisatomi, Hirotaka; Ueno, Yumiko; Taira, Shu; Sahashi, Yuko; Kawasaki, Hideya; Arakawa, Ryuichi

doi:10.2116/analsci.29.429

Cited by 4 publications

(4 citation statements)

References 27 publications

(19 reference statements)

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“…A summary of the available literature close to this eld is provided in Table S3. † 27,36,47,49,104,178,186,[191][192][193][194][195][196][197][198]203,208,[257][258][259][260][261][262][263][264] In the last decade, one-dimensional LC and LC-MS remained the most commonly used vehicle to rapidly characterize ginsenosides from ginseng. Typically, ginsenosides are extracted from ginseng materials by different concentrations of methanol (50% or 70% (ref.…”

Section: Multicomponent Characterizationmentioning

confidence: 99%

“…have been applied to identify ginsenosides or the volatile components from ginseng (including the different parts: root/rhizome, leaf, flower, berry, and seed). A summary of the available literature close to this field is provided in Table S3 † 27,36,47,49,104,178,186,191–198,203,208,257–264.…”

Section: Quality Controlmentioning

confidence: 99%

“…Typically, ginsenosides are extracted from ginseng materials by different concentrations of methanol (50% or 70% (ref. 262 and 263)) with the assistance of ultrasound. Various chromatographic columns of RPC from Waters, Agilent, YMC, etc.…”

Section: Quality Controlmentioning

confidence: 99%

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Advances and challenges in ginseng research from 2011 to 2020: the phytochemistry, quality control, metabolism, and biosynthesis

Liu

Zuo

et al. 2022

Nat. Prod. Rep.

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Section: Multicomponent Characterizationmentioning

confidence: 99%

Section: Quality Controlmentioning

confidence: 99%

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Advances and challenges in ginseng research from 2011 to 2020: the phytochemistry, quality control, metabolism, and biosynthesis

Liu

Zuo

et al. 2022

Nat. Prod. Rep.

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“…Liquid chromatography/electrospray ionization mass spectrometry (LC/ESI-MS) has been used to determine the content of nutrition in extracts of fresh samples. [16][17][18] However, this method requires the preparation of extracted samples with cumbersome pretreatment and lacs spatial information of target molecules. The advantage of MSI is that it is an easy optical method for optical type to understanding the localization of target molecules due to direct detection from a sample section that maintains spatial information.…”

Section: Concept For Food Analysis By Mass Spectrometry Imaging (Msi)mentioning

confidence: 99%

Mass Spectrometry Imaging: Applications to Food Science

et al. 2014

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Isoprenoid Production via Plant Cell Cultures: Biosynthesis, Accumulation and Scaling-Up to Bioreactors

Носов

Попова

Кочкин

2014

Production of Biomass and Bioactive Compounds Using Bioreactor Technology

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Plant cell culture is traditionally viewed as a unique artifi cially created biological system representing a heterogenous population of dedifferentiated cells. This system undergoes a continuous process of autoselection based on the intensity and stability of cell proliferation. We discuss here the details of formation and regulation of isoprenoid biosynthesis in plant cell in vitro based on literature survey and our research. Obviously, secondary metabolism differs in cell culture compared to the plant per se , because in cell culture metabolites are synthesized and compartmentalized within a single heterotrophic cell with sparse or underdeveloped vacuoles and plastids. For example, in plant cell cultures isoprenoid biosynthesis via MVA pathway was found to be more active than via plastid-localized MEP pathway. Also, it was hypothesized that cell cultures preferably produce metabolites, which promote cell proliferation and growth. Indeed, cell cultures of Dioscorea deltoidea produced mainly furostanol glycosides, which promoted cell division. Triterpene glycosides (ginsenosides) in the cell cultures of various Panax species are represented mainly by Rg-and Rb-groups. Rb ginsenosides are predominantly found as malonyl-esters that may infl uence their intracellular localization.Despite the difference in the isoprenoid composition in plant and cell culture the latter became an attractive source of phytochemicals as an alternative to plant harvesting. We provide in this chapter the guidelines to biotechnological production of plant isoprenoids using plant cell cultures and discuss the optimal methods of bioreactor-based cultivation and cryopreservation of plant cell collections.

show abstract

Two-dimensional Mapping Using Different Chromatographic Separations Coupled with Mass Spectrometry for the Analysis of Ginsenosides in Panax Ginseng Root and Callus

Cited by 4 publications

References 27 publications

Advances and challenges in ginseng research from 2011 to 2020: the phytochemistry, quality control, metabolism, and biosynthesis

Advances and challenges in ginseng research from 2011 to 2020: the phytochemistry, quality control, metabolism, and biosynthesis

Mass Spectrometry Imaging: Applications to Food Science

Isoprenoid Production via Plant Cell Cultures: Biosynthesis, Accumulation and Scaling-Up to Bioreactors

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