The prevalence of the iutA and ibeA genes in Escherichia coli isolates from severe and non-severe patients with bacteremic acute biliary tract infection is significantly different

Abstract

Background Although Escherichia coli is the most frequently isolated microorganism in acute biliary tract infections with bacteremia, data regarding its virulence are limited. Results Information on cases of bacteremia in acute biliary tract infection in a retrospective study was collected from 2013 to 2015 at a tertiary care hospital in Japan. Factors related to the severity of infection were investigated, including patient background, phylogenetic typing, and virulence factors of E. coli, such as adhesion, invasion, toxins, and iron acquisition. In total, 72 E. coli strains were identified in 71 cases, most of which primarily belonged to the B2 phylogroup (68.1%). The presence of the iutA gene (77.3% in the non-severe group, 46.4% in the severe group, P = 0.011) and the ibeA gene (9.1% in the non-severe group, and 35.7% in the severe group, P = 0.012) was significantly associated with the severity of infection. Among the patient characteristics, diabetes mellitus with organ involvement and alkaline phosphatase were different in the severe and non-severe groups. Conclusions We showed that bacteremic E. coli strains from acute biliary tract infections belonged to the virulent (B2) phylogroup. The prevalence of the iutA and ibeA genes between the two groups of bacteremia severity was significantly different.

Recurrent bacteremia with a hypermucoviscous Escherichia coli isolated from a patient with perihilar cholangiocarcinoma: insights from a comprehensive genome-based analysis

“…Interestingly, p537-20_1 also carried the iutA gene encoding the receptor of aerobactin, which is an important virulence factor for ExPEC and hypervirulent K. pneumoniae [ 52 ]. The carriage of iutA has been shown to be associated with increased mortality in E. coli , as well as K. pneumoniae bloodstream infection [ 23 , 64 ]. The possession of the iutA gene but also many other iron acquisition genes likely represents a fitness advantage in the biliary tract, due to the general iron limitation in bile [ 23 , 65 ].…”

“…The carriage of iutA has been shown to be associated with increased mortality in E. coli , as well as K. pneumoniae bloodstream infection [ 23 , 64 ]. The possession of the iutA gene but also many other iron acquisition genes likely represents a fitness advantage in the biliary tract, due to the general iron limitation in bile [ 23 , 65 ]. The co-occurrence of iutA and iucA in APEC E. coli was described before, but as characteristic of Col (V) plasmids [ 66 ].…”

“…Intestinal bacteria such as E. coli , especially ExPEC, are able to invade the biliary tract during bile stasis, resulting in an acute infection [ 21 , 22 ]. Furthermore, these severe infections are able to overcome the biliary system and thus allow E. coli to invade the bloodstream, leading to acute bacteremia [ 23 , 24 ]. Furthermore, Søgaard and colleagues stated that gastrointestinal, hepatobiliary, and urinary tract cancer may debut with E. coli community-acquired bacteremia [ 25 ].…”

Risk Factors, Antibiotic Profile, and Molecular Detection of Virulence and Antibiotic Resistance Genes of Enteric Bacteria in Diarrheic Calves in Egypt

“…According to Wani et al (2003), the Stx2 gene was more prevalent than Stx1, and both these genes were associated with the Eae gene in STEC strains. The iutA gene encodes the aerobactin siderophore ferric receptor protein, mediating siderophore uptake (Ikeda et al 2021). Enteric illnesses caused by E. coli pathotypes that cause diarrhea are suspected to involve EAST1.…”

Extraintestinal Pathogenic Escherichia coli Utilizes Surface-Located Elongation Factor G to Acquire Iron from Holo-Transferrin

“…Compared to commensal E. coli , ExPEC carries additional virulence factors to assist the bacteria in invasion, colonization, survival, and establishment of infection in the host ( 4 ). The iron acquisition system is an important virulence factor for ExPEC ( 5 ).…”