The prevalence of Listeria, Salmonella, Escherichia coli and E. coli O157:H7 on bison carcasses during processing

Li, Qiongzhen; Sherwood, Julie S.

doi:10.1016/j.fm.2003.12.006

Cited by 29 publications

(19 citation statements)

References 35 publications

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“…In a study from Mexico street food, 44% of the samples were positive for E. coli (Estrada-Garci et al 2004). Li et al (2004) have shown that 38% of bison carcasses are contaminated with E. coli. There is a possibility of non-pathogenic E. coli may acquire virulence genes by horizontal gene transfer and results in emergence of virulent strains (Ochman et al 2000).…”

Section: Resultsmentioning

confidence: 99%

Species specific PCR based detection of Escherichia coli from Indian foods

2017

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Escherichia coli is a faecal indicator and certain virotypes are known as pathogens. Therefore, detection and prevention of E. coli in food is very important. The existing rapid methods concentrate on detecting the pathogenic E. coli instead of total E. coli population. Present study evaluates the use of two molecular markers (uidA and flanking region of uspA) specific for the E. coli in combination with microbiological method for confirmation. Majority of the isolates (77%) were positive for both the genes tested. However, 22% of the E. coli isolates were positive for any one of the two primer sets [uidA (9%) and flanking region of uspA (13%)]. High levels of E. coli incidences (92% samples) were observed in beef while low occurrence (19% samples) was found in sprouts. Low percentage (7.3%) of E. coli isolates was positive for virulence genes tested (lt, ipaH, aggR, eaeA, stx1 and stx2). Two isolates were positive for stx genes. However, none of the isolates including stx positive isolates were E. coli 0157:H7. Maximum number of the E. coli (44%) isolates was characterized under phylogenetic group B2. This phylogenetic group comprises of extra intestinal and virulent E. coli strains.

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Section: Resultsmentioning

confidence: 99%

Species specific PCR based detection of Escherichia coli from Indian foods

2017

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“…Midline sponge samples were obtained from the hides of live steers before they were sent to slaughter using a sterile sponge moistened with 20 ml o f sterile water. Sponges were processed as previously described by Li et al (30). The 48 sponge samples collected from steers were placed in a sterile bag and transported within 24 h in a cooler with ice packs to the laboratory at North Dakota State University.…”

Section: Methodsmentioning

confidence: 99%

Isolation and Characterization of Shiga Toxin-Producing Escherichia coli Serogroups O26, O45, O103, O111, O113, O121, O145, and O157 Shed from Range and Feedlot Cattle from Postweaning to Slaughter

Ekiri

Landblom

Doetkott

et al. 2014

Journal of Food Protection

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Cattle are the main reservoirs for Shiga toxin-producing Escherichia coli (STEC) strains. E. coli O26, O45, O103, O111, O121, O145, and O157 are among the STEC serogroups that cause severe foodborne illness and have been declared as adulterants by the U.S. Department of Agriculture, Food Safety and Inspection Service. The objectives of this study were (i) to estimate the prevalence of non-O157 STEC and E. coli O157 in naturally infected beef cows and in steer calves at postweaning, during finishing, and at slaughter and (ii) to test non-O157 STEC isolates for the presence of virulence genes stx1, stx2, eaeA, and ehlyA. Samples were collected from study animals during multiple sampling periods and included fecal grabs, rectal swabs, and midline sponge samples. Laboratory culture, PCR, and multiplex PCR were performed to recover and identify E. coli and the virulence genes. The prevalence of non-O157 STEC (serogroups O26, O45, O103, O111, O121, O113, and O145) fecal shedding ranged from 8% (4 of 48 samples) to 39% (15 of 38 samples) in cows and 2% (1 of 47 samples) to 38% (9 of 24 samples) in steer calves. The prevalence of E. coli O157 fecal shedding ranged from 0% (0 of 38 samples) to 52% (25 of 48 samples) in cows and 2% (1 of 47 samples) to 31% (15 of 48 samples) in steer calves. In steer calves, the prevalence of non-O157 STEC and E. coli O157 was highest at postweaning, at 16% (15 of 96 samples) and 23% (22 of 96 samples), respectively. Among the 208 non-O157 STEC isolates, 79% (164 isolates) had stx1, 79% (165 isolates) had stx2, and 58% (121 isolates) had both stx1 and stx2 genes. The percentage of non-O157 STEC isolates encoding the eaeA gene was low; of the 165 isolates tested, 8 (5%) were positive for eaeA and 135 (82%) were positive for ehlyA. Findings from this study provide further evidence of non-O157 STEC shedding in beef cows and steer calves particularly at the stage of postweaning and before entry into the feedlot.

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“…The prevalence of STEC O157:H7 in bison has been reported to be as high as 42% (76). STEC O157 has also been isolated from the carcass of slaughtered bison at a prevalence of 1.13% (77). Non-O157 STEC serotypes including O45, O103, O111, O113, O121, and O145 have also been isolated from bison carcasses; however, none of these isolates possessed stx genes (78).…”

Section: Bison (Bison Bison)mentioning

confidence: 99%

Animal Reservoirs of Shiga Toxin-ProducingEscherichia coli

2014

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Shiga toxin-producing Escherichia coli (STEC) strains have been detected in a wide diversity of mammals, birds, fish, and several insects. Carriage by most animals is asymptomatic, thus allowing for dissemination of the bacterium in the environment without detection. Replication of the organism may occur in the gastrointestinal tract of some animals, notably ruminants. Carriage may also be passive or transient, without significant amplification of bacterial numbers while in the animal host. Animals may be classified as reservoir species, spillover hosts, or dead-end hosts. This classification is based on the animal's ability to (i) transmit STEC to other animal species and (ii) maintain STEC infection in the absence of continuous exposure. Animal reservoirs are able to maintain STEC infections in the absence of continuous STEC exposure and transmit infection to other species. Spillover hosts, although capable of transmitting STEC to other animals, are unable to maintain infection in the absence of repeated exposure. The large diversity of reservoir and spillover host species and the survival of the organism in environmental niches result in complex pathways of transmission that are difficult to interrupt.

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The prevalence of Listeria, Salmonella, Escherichia coli and E. coli O157:H7 on bison carcasses during processing

Cited by 29 publications

References 35 publications

Species specific PCR based detection of Escherichia coli from Indian foods

Species specific PCR based detection of Escherichia coli from Indian foods

Isolation and Characterization of Shiga Toxin-Producing Escherichia coli Serogroups O26, O45, O103, O111, O113, O121, O145, and O157 Shed from Range and Feedlot Cattle from Postweaning to Slaughter

Animal Reservoirs of Shiga Toxin-ProducingEscherichia coli

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