Species specific PCR based detection of Escherichia coli from Indian foods

Godambe, Lipika Pansare; Bandekar, Jayant R.; Shashidhar, Ravindranath

doi:10.1007/s13205-017-0784-8

Cited by 45 publications

(31 citation statements)

References 22 publications

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“…Lactose-positive, red, non-mucoid colonies were randomly selected from each sample and sub cultured onto Eosin Methylene Blue agar (EMB) (Scharlau, Spain) for biochemical confirmation. Species identity of E. coli was confirmed by standard biochemical properties followed by species-specific multiplex PCR 57 . Primers for the uidA gene and flanking region of the uspA gene were used.…”

Section: Methodsmentioning

confidence: 99%

High prevalence of mcr-1-encoded colistin resistance in commensal Escherichia coli from broiler chicken in Bangladesh

Ahmed

Das

Islam

et al. 2020

Sci Rep

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Colistin is a last-resort antimicrobial used for the treatment of human infections caused by multidrug-resistant Gram-negative bacteria. However, colistin is still widely used in intensive poultry production in Bangladesh. We aimed to investigate the dynamics and genetic diversity of colistin-resistant commensal Escherichia coli from broiler chickens. A total of 1200 E. coli strains were characterized from 20 broiler farms at three-time points along the production period. All strains were screened for mcr-1 to mcr-5 genes by a multiplex PCR, and their genetic diversity was measured by repetitive extragenic palindromic (REP)-PCR fingerprinting. Genomic diversity and characterization were performed by whole genome sequencing (WGS). Twenty-five percent of the commensal E. coli strains harbored mcr-1 genes. Frequency of mcr-1 gene detection correlated positively (odds ratio 1.71; 95% CI 0.96–3.06; p = 0.068) with the use of colistin in poultry flocks. REP-PCR profiles and WGS analysis showed diverse E. coli population carrying multiple antimicrobial resistance genes. Phylogenetic comparison of mcr-1-bearing strains recovered from this study with a global strain collection revealed wide phylogenetic relationship. This study identified a high prevalence of mcr-1 gene among genetically diverse E. coli populations from broiler chickens in Bangladesh suggesting a massive horizontal spread of mcr-1 rather than by clonal expansion.

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Section: Methodsmentioning

confidence: 99%

High prevalence of mcr-1-encoded colistin resistance in commensal Escherichia coli from broiler chicken in Bangladesh

Ahmed

Das

Islam

et al. 2020

Sci Rep

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“…A total of 18 isolates (31.5%) from the cloacal swabs, litter, and drinking water samples were positive for the presence of the flanking regions uspA gene (Figure -1). The uspA gene has been used as a DNA marker for the identification of E. coli by Chen & Griffiths (1998), and it is considered by another study (Godambe et al, 2017). Another report about the prevalence of E. coli in poultry from Egypt showed that 35% of the samples were positive for E. coli (Amer et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Detection of Tetracycline Resistance Genes among Escherichia coli Isolated from Layer and Broiler Breeders in West Java, Indonesia

Indrawati¹,

Khoirani

Setiyaningsih³

et al. 2021

Trop. Anim. Sci. J.

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A major current problem in public health is the issue of antimicrobial resistance of Escherichia coli in humans and poultry. In Indonesia, multidrug-resistant E. coli are of specific concern since such E. coli may cause public health problems in humans. The prevalence of multidrug-resistant chicken E. coli strains and the E. coli resistance genes, which are tet(A) and tet(B) genes, were investigated in the present study. A total of 57 swabs were collected from layer and broiler breeder farms in West Java, Indonesia, and used in the experiment. Eighteen isolates were identified as E. coli by the disk diffusion method. The isolates classified as drug-resistant and intermediate were then identified using PCR for the antimicrobial resistance genes. The results showed that 18 isolates of E. coli from layerbreeder and broiler-breeder farms in West Java were resistant to ampicillin (100%), nalidixic acid (94%), tetracycline (88%), oxytetracycline (83%), gentamicin (27%), and chloramphenicol (22%). PCR identification of E. coli antimicrobial-resistant genes in 18 isolates showed tet(A) and tet(B) genes. This study reports antimicrobial resistance genes among E. coli on layer and broiler breeder farms in West Java. This present study showed that E. coli isolated from layers-breeder and broiler-breeder farms in West Java of Indonesia carried tet(A) and tet(B) genes, the multidrug-resistance genes.

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“…Likewise, mycobacteria are renowned etiological agents of disease as has already been discussed. The uidA gene, on the hand, is a known molecular marker gene used to identify pathogenic Escherichia coli (Feng and Lampel 1994;Godambe et al 2017).…”

Section: Discussionmentioning

confidence: 99%

Distribution and comparison of bacterial communities in HVAC systems of two university buildings: Implications for indoor air quality and public health

Sibanda

Selvarajan

Ogola

et al. 2021

Environ Monit Assess

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The installation of HVAC systems in building is meant to enhance indoor air quality as well as increase comfort to occupants. However, HVAC systems have also become a vehicle of contamination of indoor air with potentially pathogenic microorganisms. DNA was extracted from ten HVAC filter dust samples collected from two buildings and subjected to high throughput sequencing analysis to determine the bacterial community structure. Further, the Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt2) software was used to predict the potential functional capabilities of the bacterial communities. Sequencing analysis led to the identification of five major bacterial phyla, including Proteobacteria, Cyanobacteria, Actinobacteria, Firmicutes and Bacteroidetes. At genus level, Mycobacterium, Bacillus, Cupriavidus, Hyphomicrobium and Mesorhizobium were the most dominant. With the exception of the later two bacterial genera, the first three are potential pathogens whose presence in HVAC systems poses a significant public health risk, especially among immunocompromised individuals. Nine pathways associated with antibiotics resistance and bacterial pathogenicity were identified, including polymyxin resistance and peptidoglycan biosynthesis pathways. Further, investigation of the relationship between the detected bacterial meta-communities and predicted potential virulence factors (antibiotic resistance and pathogenic genes) led to the detection of 350 positive associations among 43 core bacteria, 2 pathogenic genes (sitA and uidA) and 14 resistance genes. Overall, the heterogeneous nature of microorganisms found in HVAC systems observed in this study shows that HVAC systems are the origin of airborne infections in indoor environments, and must be periodically cleaned and disinfected to avoid the build-up of pathogens, and the subsequent exposure of human occupants of these pathogens.

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Species specific PCR based detection of Escherichia coli from Indian foods

Cited by 45 publications

References 22 publications

High prevalence of mcr-1-encoded colistin resistance in commensal Escherichia coli from broiler chicken in Bangladesh

High prevalence of mcr-1-encoded colistin resistance in commensal Escherichia coli from broiler chicken in Bangladesh

Detection of Tetracycline Resistance Genes among Escherichia coli Isolated from Layer and Broiler Breeders in West Java, Indonesia

Distribution and comparison of bacterial communities in HVAC systems of two university buildings: Implications for indoor air quality and public health

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