The prevalence and genetic diversity of porcine circovirus types 2 and 3 in Northeast China from 2015 to 2018

Xia, Deli; Huang, Liping; Xie, Yongxing; Zhang, Xiaoqian; Wei, Yanwu; Liu, Dan; Zhu, Hongzhen; Bian, Haiqiao; Feng, Li; Liu, Changming

doi:10.1007/s00705-019-04336-4

Cited by 33 publications

(35 citation statements)

References 36 publications

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“…The 5′ end of PCV3 contains 235 nucleotides (nt) and is identical to regions previously discovered in PCV1 and PCV2 [15]. Most recent phylogenetic analyses suggest that its sequences can be divided into two groups, PCV3a and PCV3b with, respectively, more than 99.1 and 97.3% nucleotide sequence identities, which can be subdivided into subgroups, including: a1, a2, b1 and b2 [6]; PCV3a, PCV3b and PCV3-IM [16]; PCV3a, PCV3b and PCV3c [17]; and PCV3a-1, PCV3a-IM, PCV3a-2 and PCV3b [18].…”

Section: Introductionmentioning

confidence: 68%

“…Fifteen genomes were isolated and sequenced in Slovenia, showing a 99.5% similarity inside its borders. Our virus strain is ~ 99.5–100% complementary to China’s MK347417 PCV3 HLJ0710 [ 16 ], MF583106 PCV3/CN/Jiangxi-3/2016 [ 18 ] and KY075994 PCV3/CN/Chongqing-156/2016 [ 26 ]. On the other hand, Faccini et al in 2017 only showed 99.2% similarity [ 27 ] when compared to the Chinese strain discovered by Ha et al [ 26 ].…”

Section: Discussionmentioning

confidence: 99%

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A molecular survey and phylogenetic analysis of porcine circovirus type 3 using oral fluid, faeces and serum

et al. 2020

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Background: Porcine circovirus type 3 is the most recently discovered porcine circovirus, and an emerging pathogen. In this study the status of its presence on some Slovenian farms is reported. The effectiveness of the vaccine against porcine circovirus type 2 was assessed against porcine circovirus type 3. Group samples of oral fluid, faeces and individual serum samples were taken from six different pig categories and tested for presence of viral DNA, using both real time and conventional PCR. Positive samples were subjected to direct Sanger sequencing. Nucleotide sequences were analyzed and compared to GenBank PCV3 sequences. Results: Positive samples were sent for genome sequencing, which confirmed the presence of virus in all different pig categories on five farms. A high to moderate correlation of strong statistical significance was found between individual serum samples, oral fluid and faeces. Slovenian PCV3 was found to be distributed in a way similar to that of other countries. Slovenian PCV3 nt sequences are highly related, sharing more than 99.5% nt identity. On one farm a commercially available vaccine against porcine circovirus type 2 was used on 3-week-old pigs. It did not affect the presence of porcine circovirus type 3 in oral fluid or sera of any of the seven age groups of pigs, each with two control groups. Conclusions: The results constitute the first discovery of the virus in Slovenia. Genome sequencing has revealed a high degree of similarity between Slovenian and GenBank isolates.

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Section: Introductionmentioning

confidence: 68%

Section: Discussionmentioning

confidence: 99%

A molecular survey and phylogenetic analysis of porcine circovirus type 3 using oral fluid, faeces and serum

et al. 2020

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“…On the contrary, PCV-2 and PCV-3 co-infections have been only assessed in studies from Asia (Kim et al, 2018;Xia et al, 2019) and only one in Europe (Woźniak, Miłek, Matyba, & Stadejek, 2019).…”

Section: Introductionmentioning

confidence: 99%

“…These co‐infections and the interaction of the mentioned pathogens with PCV‐2 have been widely studied (Allan et al., 1999; Grau‐Roma, Fraile, & Segalés, 2011; Opriessnig, Fenaux, et al., 2004; Opriessnig, Thacker, et al., 2004). On the contrary, PCV‐2 and PCV‐3 co‐infections have been only assessed in studies from Asia (Kim et al., 2018; Xia et al., 2019) and only one in Europe (Woźniak, Miłek, Matyba, & Stadejek, 2019).…”

Section: Introductionmentioning

confidence: 99%

Detection and genotyping of Porcine circovirus 2 (PCV‐2) and detection of Porcine circovirus 3 (PCV‐3) in sera from fattening pigs of different European countries

Saporiti¹,

Huerta²,

Correa‐Fiz³

et al. 2020

Transbound. Emerg. Dis.

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“…PCV1 was first found in cultured cells, and it is widely present in pigs but not pathogenic (Tischer et al 1986), and PCV2 has always been considered to be the main factor of PCV. PCV3 was first isolated from pigs with PDNS symptoms in the United States in 2016, and became popular in pig herds all over the world, such as Poland, Italy, South Korea, and China, causing huge economic losses to the pig industry (Bera et al 2020;Hayashi et al 2018;Kim et al 2018;Wang et al 2020b;Xia et al 2019;Yuzhakov et al 2018). Retrospective research data demonstrated that PCV3 possessed a high sequence homology to bat circovirus, suggesting that PCV3 might have been derived early from bats and gradually adapted to pigs (Li et al 2018).…”

Section: Discussionmentioning

confidence: 99%

Establishment and Application of Dual TaqMan Fluorescence Quantitative Polymerase Chain Reaction (PCR) for the Detection of Porcine Circovirus Types 2 and 3

Cao

Luan

Wang

et al. 2021

Preprint

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To establish a rapid method for the simultaneous differential detection of porcine circovirus types 2 (PCV2) and 3 (PCV3), two pairs of primers and two TaqMan probes were designed according to the gene sequences of PCV2 and PCV3, and a dual TaqMan fluorescence quantitative polymerase chain reaction (PCR) method for the simultaneous detection of two virus nucleic acids was established. The results showed that the correlation coefficients (R2) of the standard curves drawn using the recombinant plasmids of PCV2 and PCV3 were greater than 0.99 and had a good linear relationship. The specific detection results of PCV type 1, Porcine parvovirus, and Porcine pseudorabies virus were negative. The detection limits of this experimental method for PCV2 and PCV3 were 10 and 1 copies/μL, respectively, which were more sensitive than those of the common PCR detection methods. The established method was used to detect 76 samples from some pig farms in Shandong Province. 11 of the 76 samples were PCV3 positive (positive rate of 14.47%), 24 were PCV2 positive (positive rate of 34.58%), and PCV3 and PCV2 were mixed in six samples (positive rate of 7.89%). The whole-genome sequence of PCV3 was amplified and sequenced to further understand the molecular biological characteristics and genetic variation of PCV3 in Shandong Province. The genomes of 11 PCV3 strains were all 2000 bp long, and the whole-genome sequence homology between them ranged from 98.4% to 99.9%. There were mutation sites in the amino acid sequences of Cap and Rep proteins, and the strains isolated in this experiment were concentrated in the PCV3a and PCV3c subgroups. This study provides technical support and molecular biological basis for nucleic acid detection, epidemiological characteristics, genetic variation, and control of PCV3 in Shandong Province.

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The prevalence and genetic diversity of porcine circovirus types 2 and 3 in Northeast China from 2015 to 2018

Cited by 33 publications

References 36 publications

A molecular survey and phylogenetic analysis of porcine circovirus type 3 using oral fluid, faeces and serum

A molecular survey and phylogenetic analysis of porcine circovirus type 3 using oral fluid, faeces and serum

Detection and genotyping of Porcine circovirus 2 (PCV‐2) and detection of Porcine circovirus 3 (PCV‐3) in sera from fattening pigs of different European countries

Establishment and Application of Dual TaqMan Fluorescence Quantitative Polymerase Chain Reaction (PCR) for the Detection of Porcine Circovirus Types 2 and 3

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