Background: Porcine circovirus type 3 is the most recently discovered porcine circovirus, and an emerging pathogen. In this study the status of its presence on some Slovenian farms is reported. The effectiveness of the vaccine against porcine circovirus type 2 was assessed against porcine circovirus type 3. Group samples of oral fluid, faeces and individual serum samples were taken from six different pig categories and tested for presence of viral DNA, using both real time and conventional PCR. Positive samples were subjected to direct Sanger sequencing. Nucleotide sequences were analyzed and compared to GenBank PCV3 sequences. Results: Positive samples were sent for genome sequencing, which confirmed the presence of virus in all different pig categories on five farms. A high to moderate correlation of strong statistical significance was found between individual serum samples, oral fluid and faeces. Slovenian PCV3 was found to be distributed in a way similar to that of other countries. Slovenian PCV3 nt sequences are highly related, sharing more than 99.5% nt identity. On one farm a commercially available vaccine against porcine circovirus type 2 was used on 3-week-old pigs. It did not affect the presence of porcine circovirus type 3 in oral fluid or sera of any of the seven age groups of pigs, each with two control groups. Conclusions: The results constitute the first discovery of the virus in Slovenia. Genome sequencing has revealed a high degree of similarity between Slovenian and GenBank isolates.
The aims of the study were to assess the welfare of pigs in Slovenian farms based on the international Welfare Quality? Assessment protocol for pigs and to gain a first insight into the welfare of pigs in Slovenian conventional and alternative farms. Pig welfare in Slovenia was assessed using the Welfare Quality? protocol on 10 alternative and 10 conventional farms. The size of the farm ranged from 11 to 1900 breeding sows in conventional farms and from three to 50 breeding sows in alternative farms. Using the protocol, the welfare of breeding sows, suckling piglets, growers, and fattening pigs was evaluated. The protocol consisted of four main principles of animal welfare (good feeding, good housing, good health, and appropriate behaviour), which were subdivided into 12 independent criteria. To evaluate each of these criteria, a set of measures was used. Overall animal welfare quality was calculated with a mathematical model incorporated into the protocol. Depending on the scores of the four principles, farms were classified as excellent, enhanced, acceptable or not classified. According to the Welfare Quality? protocol and statistical calculation, growers and fatteners in Slovenian conventional farms were rated as acceptable, while Slovenian alternative farms were rated as enhanced. We can conclude that the welfare of the growers and fatteners in our alternative farms is at a higher level than in conventional farms. The most critical evaluation points in sows were bursitis, wounds on the body, stereotypies, and fear of humans.
Background: Porcine circovirus type 3 is the most recently discovered porcine circovirus, and an emerging pathogen. In this study the status of its presence on some Slovenian farms is reported. The effectiveness of the vaccine against porcine circovirus type 2 was assessed against porcine circovirus type 3.Group samples of oral fluid, faeces and individual serum samples were taken from six different pig categories and tested for presence of viral DNA, using both real time and conventional PCR. Positive samples were subjected to direct Sanger sequencing. Nucleotide sequences were analyzed and compared to GenBank PCV3 sequences.Results: Positive samples were sent for genome sequencing, which confirmed the presence of virus in all different pig categories on five farms. A high to moderate correlation of strong statistical significance was found between individual serum samples, oral fluid and faeces. Slovenian PCV3 was found to be distributed in a way similar to that of other countries. Slovenian PCV3 nt sequences are highly related, sharing more than 99.5 % nt identity. On one farm a commercially available vaccine against porcine circovirus type 2 was used on 3-week-old pigs. It did not affect the presence of porcine circovirus type 3 in oral fluid or sera of any of the seven age groups of pigs, each with two control groups.Conclusions: The results constitute the first discovery of the virus in Slovenia. Genome sequencing has revealed a high degree of similarity between Slovenian and GenBank isolates.
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