Background: Symptoms in the premenstrual period have a detrimental impact on the general health of women. There is a lack of studies examining the relationship between menstrual attitudes and symptoms of premenstrual syndrome (PMS), especially in the Indian scenario. This study was conducted to look at contributing factors, frequency of PMS in different age groups and the association of these symptoms towards the attitude towards menstruation in nursing staff. Methodology: The cross-sectional study involved 450 female nursing staff between 25-50 years of age from various tertiary care hospitals who were administered the Premenstrual Tension Syndrome Rating Scale (PMTS) and Menstrual Attitude Questionnaire (MAQ). The data was then statistically analyzed. Results: The prevalence of premenstrual symptoms was found to be 85.6% amongst the participants (according to the ACOG criteria), while the proportion of females suffering from PMS (according to DSM IVTR criteria) was 36.5%. On studying the association, a negatively significant correlation between scores on PMTS and mean scores of MAQ across all age groups. Conclusion: The intensity of PMS was associated with negative menstrual attitude in our study. Further studies on PMS, its frequent symptoms and relevant variables may guide psychoeducational interventions and need to carried out in larger and diverse populations to corroborate our findings.