The present state and perspectives of micronucleus assay in radiation protection. A review

Almássy, Zsuzsanna; Krepinsky, Alena B.; Bianco, Anna Monica; Köteles, G.J.

doi:10.1016/0883-2889(87)90033-5

Cited by 94 publications

(34 citation statements)

References 53 publications

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“…Indeed, there does not seem to be consensus on the form of the relationship between micronucleus induction and the dose of radiation. It has been variously described as conforming to a power law [6,21], a linear-quadratic expression [1,2,10,13] or a linear expression [3,[7][8][9]. In the work presented here the fit to a straight line for plots of in-vitro and in-vivo induction of micronuclei against radiation dose was excellent.…”

Section: Discussionmentioning

confidence: 87%

Assessment of the micronucleus assay as a biological dosimeter using cytokinesis-blocked lymphocytes from cancer patients receiving fractionated partial body-radiotherapy

Roux¹,

Slabbert

Smit

et al. 1998

Strahlenther Onkol

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The micronucleus assay appears to offer a reliable and consistent method for equivalent whole body radiation dose estimation, although our findings should be confirmed using lymphocytes from radiotherapy patients with tumors at anatomical sites other than the pelvis. Except at doses lower than about ).4 Gy, the method yields dose estimates acceptably close to "true" physically determined doses. The assay can be performed relatively rapidly and can be used as a "first line" biological dosimeter in situations where accidental exposure to relatively high radiation doses has occurred.

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Section: Discussionmentioning

confidence: 87%

Assessment of the micronucleus assay as a biological dosimeter using cytokinesis-blocked lymphocytes from cancer patients receiving fractionated partial body-radiotherapy

Roux¹,

Slabbert

Smit

et al. 1998

Strahlenther Onkol

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“…Preliminary measurements for blood samples irradiated with a dose of 1 Gy X-rays indicate that the automated system can find 89% f 12% of the micronuclei within the binucleated cells compared to a manual screening. Key terms: Automated micronucleus assay, ionizing radiation, dose dependence of micronucleus induction, frequency distribution of micronuclei Many recent papers describe the use of the micronucleus assay on human lymphocytes as a suitable and sensitive test to detect chromosomal damage induced by ionizing radiation or chemicals (1,10,13,18). Micronuclei (MN) in lymphocytes arise from whole chromosomes or from acentric chromosomes that are not incorporated into the daughter nuclei a t mitosis.…”

mentioning

confidence: 99%

Scoring of radiation‐induced microuclei in cytokinesis‐blocked human lymphocytes by automated image analysis

Verhaegen¹,

Vral²,

Seuntjens³

et al. 1994

Cytometry

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The micronucleus assay in human lymphocytes is, at present, frequently used to assess chromosomal damage caused by ionizing radiation or mutagens. Manual scoring of micronuclei (MN) by trained personnel is very time-consuming, tiring work, and the results depend on subjective interpretation of scoring criteria. More objective scoring can be accomplished only if the test can be automated. Furthermore, an automated system allows scoring of large numbers of cells, thereby increasing the statistical significance of the results. This is of special importance for screening programs for low doses of chromosome-damaging agents.In this paper, the first results of our effort to automate the micronucleus assay with an image-analysis system are represented. The method we used is described in detail, and the results are compared to those of other groups. Our system is able to detect 88% of the binucleated lymphocytes on the slides. The procedure consists of a fully automated localization of binucleated cells and counting of the MN within these cells, followed by a simple and fast manual operation in which the false positives are removed. Preliminary measurements for blood samples irradiated with a dose of 1 Gy X-rays indicate that the automated system can find 89% f 12% of the micronuclei within the binucleated cells compared to a manual screening.

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“…Micronuclei were observed by fluorescence microscopy using a nuclear specific fluorochrome (acridine orange) and identified using the criteria established by ALMASSY et al 16 Between 500 and 1000 cells were analysed in each sample. Binucleated cells were induced by adding cytochalasin B (2-3 gg/ml) to the growth medium during the last 24-28 hours of culture.…”

Section: Biological Endpointmentioning

confidence: 99%

Synthesis of [123I] iodoantipyrine to study the high-LET characteristics of Auger electrons in mammalian cells

Slabbert

Langenhoven

Smit

1999

J Radioanal Nucl Chem

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The present state and perspectives of micronucleus assay in radiation protection. A review

Cited by 94 publications

References 53 publications

Assessment of the micronucleus assay as a biological dosimeter using cytokinesis-blocked lymphocytes from cancer patients receiving fractionated partial body-radiotherapy

Assessment of the micronucleus assay as a biological dosimeter using cytokinesis-blocked lymphocytes from cancer patients receiving fractionated partial body-radiotherapy

Scoring of radiation‐induced microuclei in cytokinesis‐blocked human lymphocytes by automated image analysis

Synthesis of [123I] iodoantipyrine to study the high-LET characteristics of Auger electrons in mammalian cells

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