The presence of large amounts of type III collagen in bovine dental pulp and its significance with regard to the mechanism of dentinogenesis

Lechner, Joseph H.; Kalnitsky, George

doi:10.1016/0003-9969(81)90046-7

Cited by 25 publications

(7 citation statements)

References 43 publications

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“…However, for the rest of the life of the tooth, there is no change in collagen content (Nielsen et al 1983). Our data suggest that all the pulp collagen, viz., types I, III, V, and VI, remains in the pulp during aging but is compressed into a smaller volume as the pulp cavity shrinks during dentinogenesis (Lechner and Kalnitsky 1981). The increasing fibril density in the pulp during dentinogenesis confirms the hypothesis of Stanley and Ranney (1962).…”

Section: Discussionmentioning

confidence: 78%

Light-microscopical investigation of the distribution of extracellular matrix molecules and calcifications in human dental pulps of various ages

Hillmann

Geurtsen

1997

Cell and Tissue Research

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The distribution of extracellular matrix molecules, especially collagen types I, III, V, and VI, in the extracellular matrix of the connective tissue of human dental pulp of various ages was studied by polarization and indirect immunofluorescence microscopy by using a conventional fluorescence microscope and a confocal laser scanning microscope. Polarization and immunofluorescence microscopy of paraffin sections showed thick fibers of collagen type I, which represented the main component of the connective tissue matrix of the dental pulp. By indirect immunofluorescence, thin fibers and small bundles of collagen type III were determined to be one of the main fibrillar elements present in the dental pulp matrix. Collagen type IV was detected by a clear intense staining of the basement membrane of blood vessels at all ages examined. Collagens type V and VI formed a dense meshwork of thin microfibrils throughout the stroma of the connective tissue of the dental pulp. These fibers were localized around blood vessels and appeared to be enriched in the subodontoblastic layer. Investigations by means of confocal laser scanning microscopy revealed fibers of collagen type VI spiralling between fully differentiated odontoblasts toward the predentin layer. With advancing age, the connective tissue matrix appeared to be condensed and aggregates of thick fiber bundles could be observed. Furthermore, the participation of various collagen types in the composition of pulp stones was shown. These calcifications and diffuse calcifications increased in frequency with advancing age in a statistically significant manner.

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Section: Discussionmentioning

confidence: 78%

Light-microscopical investigation of the distribution of extracellular matrix molecules and calcifications in human dental pulps of various ages

Hillmann

Geurtsen

1997

Cell and Tissue Research

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“…Of the 13 types described, types I, 11, 111, V, and XI are fibrillar (Tikka et al, 1988). The available evidence indicates that dentin and predentin contain type I collagen only (e.g., Takita et al, 19871, whereas the pulp contains mostly type I, up to 45% type I11 (Lechner and Kalnitsky, 1981), and small amounts of type V (2%, Tsuzaki et al, 1990). We made a few measurements of the diameters of predentinal collagen fibrils which were not obviously connected to the von Korff bundles, and they were very similar to the 30-40-nm diameters within the von Korff fibers.…”

Section: Resultsmentioning

confidence: 97%

Interodontoblastic collagen (von Korff fibers) and circumpulpal dentin formation: An Ultrathin serial section study in the cat

1991

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The collagenous fibers of von Korff pass from the dentin matrix between the odontoblasts into the dental pulp. Although collagen fibrils are known to be present between odontoblasts, the existence of von Korff fibers has remained controversial. This may be because their continuity between the dentin matrix and the pulp has not been demonstrated ultrastructurally. In this study we have examined the odontoblast layer in the middle to apical regions of perfusion-fixed permanent canine teeth of cats by using transmission electron microscopy. Ultrathin sections of demineralized specimens revealed frequent bundles of collagen fibrils 1) entering the odontoblast layer from the predentin, 2) present between odontoblast cell bodies, and 3) passing from between the odontoblasts into the pulp. The question of continuity of these bundles from the predentin, across the odontoblast layer into the pulp was examined in ultrathin serial sections. Unbroken continuity of a collagen bundle from the predentin between the odontoblasts into the pulp was established in a reconstruction of one series of 22 serial sections and was very strongly suggested by a number of other series in which the numbers of available sections restricted their full visibility. This investigation has shown, therefore, that classical von Korff fibers are present and that these fibers are present in fully erupted teeth with closed apices, i.e., at a time when secondary circumpulpal dentinogenesis is in progress. The findings call for a reexamination of the question of von Korff fibers during mantle dentinogenesis and primary circumpulpal dentinogenesis. Resolution of their existence at the earlier stages of dentinogenesis should be possible by using the ultrathin serial-sectioning technique.

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“…Dentine and predentine contain only type I collagen (Takita et al . 1987) whereas the pulp contains mostly type I, with up to 45% type III (Lechner & Kalnitsky 1981) and smaller amounts of type V collagen (Tsuzaki et al . 1990).…”

Section: Discussionmentioning

confidence: 99%

Location, arrangement and possible function of interodontoblastic collagen fibres in association with calcium hydroxide-induced hard tissue bridges

Kitasako¹,

Shibata²,

Cox³

et al. 2002

International Endodontic Journal

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The presence of large amounts of type III collagen in bovine dental pulp and its significance with regard to the mechanism of dentinogenesis

Cited by 25 publications

References 43 publications

Light-microscopical investigation of the distribution of extracellular matrix molecules and calcifications in human dental pulps of various ages

Light-microscopical investigation of the distribution of extracellular matrix molecules and calcifications in human dental pulps of various ages

Interodontoblastic collagen (von Korff fibers) and circumpulpal dentin formation: An Ultrathin serial section study in the cat

Location, arrangement and possible function of interodontoblastic collagen fibres in association with calcium hydroxide-induced hard tissue bridges

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